Ruminococcus bromii, identification and isolation as a dominant community member in the rumen of cattle fed a barley diet
- PMID: 18045390
- DOI: 10.1111/j.1365-2672.2007.03492.x
Ruminococcus bromii, identification and isolation as a dominant community member in the rumen of cattle fed a barley diet
Abstract
Aims: To identify dominant bacteria in grain (barley)-fed cattle for isolation and future use to increase the efficiency of starch utilization in these cattle.
Methods and results: Total DNA was extracted from samples of the rumen contents from eight steers fed a barley diet for 9 and 14 days. Bacterial profiles were obtained using denaturing gradient gel electrophoresis (DGGE) of the PCR-amplified V2/V3 region of the 16S rRNA genes from total bacterial DNA. Apparently dominant bands were excised and cloned, and the clone insert sequence was determined. One of the most common and dominant bacteria present was identified as Ruminococcus bromii. This species was subsequently isolated using traditional culture-based techniques and its dominance in the grain-fed cattle was confirmed using a real-time Taq nuclease assay (TNA) designed for this purpose. In some animals, the population of R. bromii reached densities above 10(10)R. bromii cell equivalents per ml or approximately 10% of the total bacterial population.
Conclusions: Ruminococcus bromii is a dominant bacterial population in the rumen of cattle fed a barley-based diet.
Significance and impact of the study: Ruminococcus bromii YE282 may be useful as a probiotic inoculant to increase the efficiency of starch utilization in barley-fed cattle. The combination of DGGE and real-time TNA has been an effective process for identifying and targeting for isolation, dominant bacteria in a complex ecosystem.
Similar articles
-
Characterisation of prototype Nurmi cultures using culture-based microbiological techniques and PCR-DGGE.Int J Food Microbiol. 2006 Aug 1;110(3):268-77. doi: 10.1016/j.ijfoodmicro.2006.04.028. Epub 2006 Jul 11. Int J Food Microbiol. 2006. PMID: 16814892
-
Phylotypes related to Ruminococcus bromii are abundant in the large bowel of humans and increase in response to a diet high in resistant starch.FEMS Microbiol Ecol. 2008 Dec;66(3):505-15. doi: 10.1111/j.1574-6941.2008.00527.x. Epub 2008 Jun 24. FEMS Microbiol Ecol. 2008. PMID: 18616586 Clinical Trial.
-
16S ribosomal RNA-based methods to monitor changes in the hindgut bacterial community of piglets after oral administration of Lactobacillus sobrius S1.Anaerobe. 2008 Apr;14(2):78-86. doi: 10.1016/j.anaerobe.2007.12.004. Epub 2008 Jan 5. Anaerobe. 2008. PMID: 18272412 Clinical Trial.
-
The use of molecular techniques based on ribosomal RNA and DNA for rumen microbial ecosystem studies: a review.Mol Biol Rep. 2008 Jun;35(2):265-74. doi: 10.1007/s11033-007-9079-1. Epub 2007 May 5. Mol Biol Rep. 2008. PMID: 17484038 Review.
-
The Ruminococci: key symbionts of the gut ecosystem.J Microbiol. 2018 Mar;56(3):199-208. doi: 10.1007/s12275-018-8024-4. Epub 2018 Feb 28. J Microbiol. 2018. PMID: 29492877 Review.
Cited by
-
Understanding the Diversity and Roles of the Ruminal Microbiome.J Microbiol. 2024 Mar;62(3):217-230. doi: 10.1007/s12275-024-00121-4. Epub 2024 Apr 25. J Microbiol. 2024. PMID: 38662310 Review.
-
Increasing buffering capacity enhances rumen fermentation characteristics and alters rumen microbiota composition of high-concentrate fed Hanwoo steers.Sci Rep. 2022 Dec 1;12(1):20739. doi: 10.1038/s41598-022-24777-3. Sci Rep. 2022. PMID: 36456638 Free PMC article.
-
Susceptibility of dairy cows to subacute ruminal acidosis is reflected in both prepartum and postpartum bacteria as well as odd- and branched-chain fatty acids in feces.J Anim Sci Biotechnol. 2022 Oct 5;13(1):87. doi: 10.1186/s40104-022-00738-8. J Anim Sci Biotechnol. 2022. PMID: 36195941 Free PMC article.
-
Changes in Rumen Bacterial Community Induced by the Dietary Physically Effective Neutral Detergent Fiber Levels in Goat Diets.Front Microbiol. 2022 Apr 11;13:820509. doi: 10.3389/fmicb.2022.820509. eCollection 2022. Front Microbiol. 2022. PMID: 35479630 Free PMC article.
-
Heat Stress: Effects on Rumen Microbes and Host Physiology, and Strategies to Alleviate the Negative Impacts on Lactating Dairy Cows.Front Microbiol. 2022 Feb 28;13:804562. doi: 10.3389/fmicb.2022.804562. eCollection 2022. Front Microbiol. 2022. PMID: 35295316 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials