Characterization of keratins and associated proteins involved in the corneification of crocodilian epidermis
- PMID: 17707449
- DOI: 10.1016/j.tice.2007.06.004
Characterization of keratins and associated proteins involved in the corneification of crocodilian epidermis
Abstract
Crocodilian keratinocytes accumulate keratin and form a corneous cell envelope of which the composition is poorly known. The present immunological study characterizes the molecular weight, isoelectric point (pI) and the protein pattern of alpha- and beta-keratins in the epidermis of crocodilians. Some acidic alpha-keratins of 47-68 kDa are present. Cross-reactive bands for loricrin (70, 66, 55 kDa), sciellin (66, 55-57 kDa), and filaggrin-AE2-positive keratins (67, 55 kDa) are detected while caveolin is absent. These proteins may participate in the formation of the cornified cell membranes, especially in hinge regions among scales. Beta-keratins of 17-20 kDa and of prevalent basic pI (7.0-8.4) are also present. Acidic beta-keratins of 10-16 kDa are scarce and may represent altered forms of the original basic proteins. Crocodilian beta-keratins are not recognized by a lizard beta-keratin antibody (A68B), and by a turtle beta-keratin antibody (A685). This result indicates that these antibodies recognize specific epitopes in different reptiles. Conversely, crocodilian beta-keratins cross-react with the Beta-universal antibody indicating they share a specific 20 amino acid epitope with avian beta-keratins. Although crocodilian beta-keratins are larger proteins than those present in birds our results indicate presence of shared epitopes between avian and crocodilian beta-keratins which give good indication for the future determination of the sequence of these proteins.
Similar articles
-
Characterization of beta-keratins in lizard epidermis: electrophoresis, immunocytochemical and in situ-hybridization study.Tissue Cell. 2007 Feb;39(1):1-11. doi: 10.1016/j.tice.2006.10.001. Epub 2006 Nov 13. Tissue Cell. 2007. PMID: 17101163
-
Immuno-cross reactivity of transglutaminase and cornification marker proteins in the epidermis of vertebrates suggests common processes of soft cornification across species.J Exp Zool B Mol Dev Evol. 2004 Nov 15;302(6):526-49. doi: 10.1002/jez.b.21016. J Exp Zool B Mol Dev Evol. 2004. PMID: 15468051
-
Localization and characterization of specific cornification proteins in avian epidermis.Cells Tissues Organs. 2004;178(4):204-15. doi: 10.1159/000083732. Cells Tissues Organs. 2004. PMID: 15812148
-
Cytochemical, biochemical and molecular aspects of the process of keratinization in the epidermis of reptilian scales.Prog Histochem Cytochem. 2006;40(2):73-134. doi: 10.1016/j.proghi.2006.01.001. Epub 2006 Mar 14. Prog Histochem Cytochem. 2006. PMID: 16584938 Review.
-
Structural and immunocytochemical characterization of keratinization in vertebrate epidermis and epidermal derivatives.Int Rev Cytol. 2006;253:177-259. doi: 10.1016/S0074-7696(06)53005-0. Int Rev Cytol. 2006. PMID: 17098057 Review.
Cited by
-
Cellular structure of dinosaur scales reveals retention of reptile-type skin during the evolutionary transition to feathers.Nat Commun. 2024 May 21;15(1):4063. doi: 10.1038/s41467-024-48400-3. Nat Commun. 2024. PMID: 38773066 Free PMC article.
-
Using Crocodylians for monitoring mercury in the tropics.Ecotoxicology. 2023 Oct;32(8):977-993. doi: 10.1007/s10646-023-02703-1. Epub 2023 Oct 10. Ecotoxicology. 2023. PMID: 37815690 Free PMC article. Review.
-
Alpha-Keratin, Keratin-Associated Proteins and Transglutaminase 1 Are Present in the Ortho- and Parakeratinized Epithelium of the Avian Tongue.Cells. 2022 Jun 11;11(12):1899. doi: 10.3390/cells11121899. Cells. 2022. PMID: 35741029 Free PMC article.
-
The evolution of the pectoral extrinsic appendicular and infrahyoid musculature in theropods and its functional and behavioral importance.J Anat. 2020 Nov;237(5):870-889. doi: 10.1111/joa.13256. Epub 2020 Aug 13. J Anat. 2020. PMID: 32794182 Free PMC article.
-
Immunohistochemical staining patterns of alpha-keratins in normal tissues from two reptile species: implications for characterization of squamous cell carcinomas.BMC Vet Res. 2018 Jul 6;14(1):219. doi: 10.1186/s12917-018-1545-6. BMC Vet Res. 2018. PMID: 29980226 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous
