doi: 10.1016/S0076-6879(05)99051-4.
Ubiquitin fusion technique and related methods
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- PMID: 16338395
- DOI: 10.1016/S0076-6879(05)99051-4
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Ubiquitin fusion technique and related methods
Methods Enzymol.
2005.
Abstract
The ubiquitin fusion technique, developed in 1986, is still the method of choice for producing a desired N-terminal residue in a protein of interest in vivo. This technique is also used as a tool for protein expression. Over the past two decades, several otherwise unrelated methods were invented that have in common the use of ubiquitin fusions as a component of design. I describe the original ubiquitin fusion technique, its current applications, and other methods that use the properties of ubiquitin fusions.
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