Mitochondrial regular arrangement in muscle cells: a "crystal-like" pattern
- PMID: 15496480
- DOI: 10.1152/ajpcell.00281.2004
Mitochondrial regular arrangement in muscle cells: a "crystal-like" pattern
Abstract
The aim of this work was to characterize quantitatively the arrangement of mitochondria in heart and skeletal muscles. We studied confocal images of mitochondria in nonfixed cardiomyocytes and fibers from soleus and white gastrocnemius muscles of adult rats. The arrangement of intermyofibrillar mitochondria was analyzed by estimating the densities of distribution of mitochondrial centers relative to each other (probability density function). In cardiomyocytes (1,820 mitochondrial centers marked), neighboring mitochondria are aligned along a rectangle, with distance between the centers equal to 1.97 +/- 0.43 and 1.43 +/- 0.43 microm in the longitudinal and transverse directions, respectively. In soleus (1,659 mitochondrial centers marked) and white gastrocnemius (621 pairs of mitochondria marked), mitochondria are mainly organized in pairs at the I-band level. Because of this organization, there are two distances characterizing mitochondrial distribution in the longitudinal direction in these muscles. The distance between mitochondrial centers in the longitudinal direction within the same I band is 0.91 +/- 0.11 and 0.61 +/- 0.07 microm in soleus and white gastrocnemius, respectively. The distance between mitochondrial centers in different I bands is approximately 3.7 and approximately 3.3 microm in soleus and gastrocnemius, respectively. In the transverse direction, the mitochondria are packed considerably closer to each other in soleus than in white gastrocnemius, with the distance equal to 0.75 +/- 0.22 microm in soleus and 1.09 +/- 0.41 microm in gastrocnemius. Our results show that intermyofibrillar mitochondria are arranged in a highly ordered crystal-like pattern in a muscle-specific manner with relatively small deviation in the distances between neighboring mitochondria. This is consistent with the concept of the unitary nature of the organization of the muscle energy metabolism.
Similar articles
-
The cell-type specificity of mitochondrial dynamics.Int J Biochem Cell Biol. 2009 Oct;41(10):1928-39. doi: 10.1016/j.biocel.2009.03.007. Epub 2009 Mar 27. Int J Biochem Cell Biol. 2009. PMID: 19703655 Review.
-
Three-dimensional mitochondrial arrangement in ventricular myocytes: from chaos to order.Am J Physiol Cell Physiol. 2006 Dec;291(6):C1148-58. doi: 10.1152/ajpcell.00236.2006. Epub 2006 Jul 5. Am J Physiol Cell Physiol. 2006. PMID: 16822946
-
Mitochondrial subpopulations and heterogeneity revealed by confocal imaging: possible physiological role?Biochim Biophys Acta. 2006 May-Jun;1757(5-6):686-91. doi: 10.1016/j.bbabio.2006.03.014. Epub 2006 Apr 17. Biochim Biophys Acta. 2006. PMID: 16712778
-
Possible role of cytoskeleton in intracellular arrangement and regulation of mitochondria.Exp Physiol. 2003 Jan;88(1):175-90. doi: 10.1113/eph8802511. Exp Physiol. 2003. PMID: 12525866 Review.
-
Study of regulation of mitochondrial respiration in vivo. An analysis of influence of ADP diffusion and possible role of cytoskeleton.Biochim Biophys Acta. 1997 Nov 10;1322(1):41-59. doi: 10.1016/s0005-2728(97)00071-6. Biochim Biophys Acta. 1997. PMID: 9398078
Cited by
-
Quantifying nanoscopic alterations associated with mitochondrial dysfunction using three-dimensional single-molecule localization microscopy.Biomed Opt Express. 2024 Feb 12;15(3):1571-1584. doi: 10.1364/BOE.510351. eCollection 2024 Mar 1. Biomed Opt Express. 2024. PMID: 38495683 Free PMC article.
-
3D reconstruction of murine mitochondria reveals changes in structure during aging linked to the MICOS complex.Aging Cell. 2023 Dec;22(12):e14009. doi: 10.1111/acel.14009. Epub 2023 Nov 13. Aging Cell. 2023. PMID: 37960952 Free PMC article.
-
Toward Ameliorating Insulin Resistance: Targeting a Novel PAK1 Signaling Pathway Required for Skeletal Muscle Mitochondrial Function.Antioxidants (Basel). 2023 Aug 22;12(9):1658. doi: 10.3390/antiox12091658. Antioxidants (Basel). 2023. PMID: 37759961 Free PMC article.
-
Three-dimensional mitochondria reconstructions of murine cardiac muscle changes in size across aging.Am J Physiol Heart Circ Physiol. 2023 Nov 1;325(5):H965-H982. doi: 10.1152/ajpheart.00202.2023. Epub 2023 Aug 25. Am J Physiol Heart Circ Physiol. 2023. PMID: 37624101 Free PMC article.
-
Structural Analysis of Mitochondria in Cardiomyocytes: Insights into Bioenergetics and Membrane Remodeling.Curr Issues Mol Biol. 2023 Jul 21;45(7):6097-6115. doi: 10.3390/cimb45070385. Curr Issues Mol Biol. 2023. PMID: 37504301 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources