Microscopy articles within Nature Communications

Featured

  • Article
    | Open Access

    Artificial stacking of van der Waals materials is an effective method to design and investigate emergent physical properties in condensed matter systems. Here, the authors characterize the natural twisted layer structure of CrI3, showing its dependence on the sample fabrication process and its implications for the magnetic properties of the material.

    • Myeongjin Jang
    • , Sol Lee
    •  & Kwanpyo Kim

  • Article
    | Open Access

    Traditional TIRF illumination is hampered by lack of precise quantification of single-molecule intensities. Here the authors combine flat-field illumination by using a standard πShaper with multi-angular TIR illumination by incorporating a spatial light modulator compatible with fast super-resolution structured illumination microscopy.

    • Hauke Winkelmann
    • , Christian P. Richter
    •  & Rainer Kurre

  • Article
    | Open Access

    Mapping of neuronal circuitry is challenging, particularly when neurons are densely packed. Here, the authors show stochastic multicolour labelling of neurons with seven fluorescent proteins. They also present an automated neurite reconstruction pipeline based on unsupervised clustering of colour information.

    • Marcus N. Leiwe
    • , Satoshi Fujimoto
    •  & Takeshi Imai

  • Article
    | Open Access

    The authors develop overtone photothermal microscopy that leverages a pump-probe detection of second overtone vibrations within the shortwave-infrared (SWIR) window. This technique complements existing large-scale SWIR imaging approaches, offering enhanced resolution and sensitivity for bioimaging applications.

    • Le Wang
    • , Haonan Lin
    •  & Ji-Xin Cheng

  • Article
    | Open Access

    Detection of membrane potential changes using voltage indicators typically requires fast imaging rates and highly sensitive imaging methods. Here, the authors introduce scanless two-photon imaging, an approach which enables high signal to noise ratio voltage recordings at kilohertz rates, from multiple neurons simultaneously, both in vitro and in vivo.

    • Ruth R. Sims
    • , Imane Bendifallah
    •  & Valentina Emiliani

  • Article
    | Open Access

    The authors propose a method for de-scanning the axial focus movement in the detection arm of a fluorescence microscope, enabling aberration-free, multi-color, volumetric imaging. They acquire dual-colour image stacks with an axial range of 70 μm and camera-limited acquisition speed.

    • Hassan Dibaji
    • , Ali Kazemi Nasaban Shotorban
    •  & Tonmoy Chakraborty

  • Article
    | Open Access

    Implementing point spread function (PSF) engineering in high-throughput microscopy has proved challenging. Here, the authors propose a compact PSF engineering approach, which allows for enhanced depth of field and for the recovery of 3D information using single snapshots.

    • Nadav Opatovski
    • , Elias Nehme
    •  & Yoav Shechtman

  • Article
    | Open Access

    Palladium-based catalysts are highly effective for the complete oxidation of methane. Here, the authors employ operando transmission electron microscopy, near-ambient pressure X-ray photoelectron spectroscopy, and density functional theory calculations to investigate the active state and catalytic function of Pd nanoparticles in methane oxidation.

    • Shengnan Yue
    • , C. S. Praveen
    •  & Xing Huang

  • Article
    | Open Access

    The authors introduce a highspeed acquisition technique, sHAPR, for rapid exploration of biodynamics using fluorescence microscopy. The method leverages sCMOS cameras and custom fibre optics to convert microscopy images into 1D recordings, enabling acquisition at the maximum camera readout rate.

    • Biagio Mandracchia
    • , Corey Zheng
    •  & Shu Jia

  • Article
    | Open Access

    The structure of the Golgi and the localization of glycosylation enzymes remain largely elusive. Here, the authors use super-resolution microscopy to show that the Golgi is composed of small dynamic units which have rapidly moving zones of glycosylation enzymes.

    • Akihiro Harada
    • , Masataka Kunii
    •  & Akihiko Nakano

  • Article
    | Open Access

    Controlled manipulation of cultured cells by delivery of exogenous macromolecules is a cornerstone of experimental biology. Here, the authors describe a platform to deliver defined numbers of macromolecules into cultured cell lines at single molecule resolution.

    • Chalmers C. Chau
    • , Christopher M. Maffeo
    •  & Paolo Actis

  • Article
    | Open Access

    Biomolecular condensates form via phase separation of multivalent macromolecules. Phase separation is governed by solubility whereas multivalence drives percolation, also known as gelation. The authors in this work identify the distinct energy and length scales that influence phase separation versus percolation.

    • Mrityunjoy Kar
    • , Laura T. Vogel
    •  & Rohit V. Pappu

  • Article
    | Open Access

    Biosensing tools to detect multiple analytes in a high-throughput manner are still hindered by many limitations. Here, the authors present a label-free optofluidic platform integrating digital holography and microfluidics for analyte detection, allowing for the fingerprinting of heterogenous biological samples.

    • Alexia Stollmann
    • , Jose Garcia-Guirado
    •  & Romain Quidant

  • Article
    | Open Access

    Previous measurements of FeSe0.45Te0.55 found one-dimensional (1D) defects that were interpretated as domain walls hosting propagating Majorana topological modes. Here, the authors reveal that these 1D defects correspond to sub-surface debris and show that the filling of the superconducting gap on these defects is topologically trivial.

    • A. Mesaros
    • , G. D. Gu
    •  & F. Massee

  • Article
    | Open Access

    Abbe’s diffraction limit has been a defining concept for microscopy. With finite photon, photon noise remains one essential factor yet to be considered in the theoretical resolution limit. Here, the authors introduced information-based resolution limit allowing for photon-considered resolution assessment of various microscopy and super-resolution modalities.

    • Yilun Li
    •  & Fang Huang

  • Article
    | Open Access

    The authors characterize the phonon modes at the FeSe/SrTiO3 interface with atomically resolved electron energy loss spectroscopy and correlate them with accurate atomic structure in an electron microscope. They find several phonon modes highly localized at the interface, one of which engages in strong interactions with the electrons in FeSe.

    • Ruochen Shi
    • , Qize Li
    •  & Peng Gao

  • Article
    | Open Access

    Kekulé vortices in hexagonal lattices can host fractionalized charges at zero magnetic field, but have remained out of experimental reach. Here, the authors report a Kekulé vortex in the local density states of graphene around a chemisorbed hydrogen adatom.

    • Yifei Guan
    • , Clement Dutreix
    •  & Vincent T. Renard

  • Article
    | Open Access

    Bioimaging with photocontrol and multiplexing capability is vital for studying cellular interactions and dynamics, but multiplexed stimulated Raman scattering (SRS) imaging with reversible photocontrol is elusive. Here, the authors report SRS microscopy with Carbow-switch enabling multiplexed SRS imaging and tracking in live cells with reversible photocontrol and high spatiotemporal selectivity.

    • Yueli Yang
    • , Xueyang Bai
    •  & Fanghao Hu

  • Article
    | Open Access

    Application of correlative light and electron microscopy (CLEM) in plants remains challenging. Here, the authors use Click-iT chemistry as a tool for CLEM, due to its unique properties in resin permeability and super-resolution microscopy. They use this approach to study cellular physiology in Arabidopsis.

    • Michal Franek
    • , Lenka Koptašíková
    •  & Jíří Fajkus

  • Article
    | Open Access

    Quickly acquiring topographical information from a sample remains a challenge in optics. Here, the authors introduce encoded search focal scan, a technique for sub-micrometric imaging of tens of topographies per second based on collecting a reduced set of images.

    • Narcís Vilar
    • , Roger Artigas
    •  & Guillem Carles

  • Article
    | Open Access

    Current approaches for volumetric super-resolution microscopy can yield large and complex PSF spatial footprints. Here, the authors show a super-resolution microscopy approach using a hexagonal microlens array, which offers speed improvements in volumetric imaging compared to other single-molecule methods.

    • Sam Daly
    • , João Ferreira Fernandes
    •  & Steven F. Lee

  • Article
    | Open Access

    There is a lack of universal tools to analyse protein assemblies and quantify underlying structures in single-molecule localization microscopy. Here, the authors present SEMORE, a semi-automatic machine learning framework for system- and input-dependent analysis of super-resolution data.

    • Steen W. B. Bender
    • , Marcus W. Dreisler
    •  & Nikos S. Hatzakis

  • Article
    | Open Access

    The researchers showcase swept-coded aperture real-time femtophotography—an all-optical single-shot computational imaging modality at up to 156.3 trillion frames per second—video-records transient absorption in a semiconductor and ultrafast demagnetization of a metal alloy.

    • Jingdan Liu
    • , Miguel Marquez
    •  & Jinyang Liang

  • Article
    | Open Access

    Researchers developed an open-hardware structured illumination microscopy add-on. This affordable upgrade provides super-resolution capabilities for normal optical microscopes. Detailed instructions enable easy reproduction to help democratize advanced microscopy.

    • Mélanie T. M. Hannebelle
    • , Esther Raeth
    •  & Georg E. Fantner

  • Article
    | Open Access

    High and medium-entropy alloys have shown excellent mechanical performance, yet the role of short-range order (SRO) on these properties has been unclear. Here, the authors demonstrate that the reduction of SRO by deformation leads to rejuvenation, explaining their remarkable damage tolerance.

    • Yang Yang
    • , Sheng Yin
    •  & Andrew M. Minor

  • Article
    | Open Access

    The GRIN lenses widely used for deep brain functional imaging suffer from a small measurement field of view due to strong fourth-order astigmatism. Here the authors report Geometric Transformation Adaptive Optics (GTAO) that corrects field-dependent astigmatism and enables large-volume in vivo imaging of deep mouse brain through 0.5 mm GRIN lenses.

    • Yuting Li
    • , Zongyue Cheng
    •  & Meng Cui

  • Article
    | Open Access

    By combining real and diffraction space data recorded in electron microscopes, ptychography retrieves specimen details with super-resolution. Here, the inverse problem is solved in the presence of thermal diffuse scattering and applied to measure ferroelectric displacements with picometer precision.

    • Benedikt Diederichs
    • , Ziria Herdegen
    •  & Knut Müller-Caspary

Browse broader subjects

Browse narrower subjects

Browse Microscopy across other nature.com journals