Extended Data Fig. 1: Gating strategies for different B cell subsets.
![Extended Data Fig. 1](https://cdn.statically.io/img/media.springernature.com/full/springer-static/esm/art%3A10.1038%2Fs41590-024-01900-2/MediaObjects/41590_2024_1900_Fig8_ESM.jpg)
a, FACS plots to gate PC (B220loCD138hi), GC (B220hiCD138loFAShiGL7hiCD38lo), antigen-binding MBC (B220hiCD138loGL7loIgDloCD38+ NIP-binding), naïve B cells (B220hiCD138loGL7loIgDhi). The CD38 level used to gate MBCs is according to the CD38 level expressed by naïve B cells. The NIP-binding gate is set according to the unimmunized control. b, Representative FACS plots of naïve B cell, GC and MBC showing RFP and EYFP fluorescence intensities of the BARBE reporter or FMO controls of Blimp-1 EYFP or Bach2tdRFP/+ reporter.