Extended Data Fig. 5: Inducible BACH2 and BLIMP-1 expression by retroviral transduction.
![Extended Data Fig. 5](https://cdn.statically.io/img/media.springernature.com/full/springer-static/esm/art%3A10.1038%2Fs41590-024-01900-2/MediaObjects/41590_2024_1900_Fig12_ESM.jpg)
a, Diagrams of the MSCV construct for BACH2 or BLIMP-1 induction (left) and relevant alleles in Cd79cre/+;Rosa26LSL-rtTA3 mice. b, The experimental design. c, A representative FACS plot for gating the mCherry+ induced and mCherry- uninduced cells. d, Representative FACS profiles of secondary PCs or GCs derived from GFP+ B1-8hi donor cells that were uninduced (top, mCherry-) or underwent successful induction of BACH2 or control mCherry or BLIMP-1 (bottom, mCherry+). e, GC% and PC% formed by MBCs that did not (top two, mCherry-) or did undergo successful BACH2 or control mCherry or BLIMP-1 induction (bottom two, mCherry+). This calculation minimizes effects caused by different induction efficiencies (indicated by mCherry signals) in order to compare those with induced expression and those without. f, GC% and PC% in total infected cells formed by MBCs that did not (top two, mCherry-) or did undergo successful BACH2 or control mCherry or BLIMP-1 induction (bottom two, mCherry+). e, f, Data were pooled from 3 independent experiments. Each symbol indicates one mouse (n=9 per group), and lines denote means. P-values by two-sided Mann-Whitney test.