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16S rRNA Amplicon Sequencing
The 16S rRNA amplicon sequencing technique is a microbiome analysis where different samples are analyzed at the same time using multiplexing. The...
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Identification of Bacterial Endophytes by 16S rRNA
Phenotypic profiles for microbial identification are unusual for rare, slow-growing, and fastidious microorganisms. The genotypic identification of...
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16S-rRNA-Amplicon-Sequenzierung für Metagenomik
Die 16S-rRNA-Amplicon-Sequenzierungstechnik ist eine Mikrobiomanalyse, bei der verschiedene Proben gleichzeitig mit Hilfe von Multiplexing analysiert...
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A comparison between full-length 16S rRNA Oxford nanopore sequencing and Illumina V3-V4 16S rRNA sequencing in head and neck cancer tissues
Describing the microbial community within the tumour has been a key aspect in understanding the pathophysiology of the tumour microenvironment. In...
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Targeted 16S rRNA Gene Capture by Hybridization and Bioinformatic Analysis
Next-generation sequencing technologies have impressively unlocked capacities to depict the complexity of microbial communitiesMicrobial communities....
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METASEED: a novel approach to full-length 16S rRNA gene reconstruction from short read data
BackgroundWith the emergence of Oxford Nanopore technology, now the on-site sequencing of 16S rRNA from environments is available. Due to the error...
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Optimized bacterial community characterization through full-length 16S rRNA gene sequencing utilizing MinION nanopore technology
BackgroundAccurate identification of bacterial communities is crucial for research applications, diagnostics, and clinical interventions. Although...
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Accounting for 16S rRNA copy number prediction uncertainty and its implications in bacterial diversity analyses
16S rRNA gene copy number (16S GCN) varies among bacterial species and this variation introduces potential biases to microbial diversity analyses...
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Exploring bacterial diversity in Arctic fjord sediments: a 16S rRNA–based metabarcoding portrait
The frosty polar environment houses diverse habitats mostly driven by psychrophilic and psychrotolerant microbes. Along with traditional cultivation...
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Full-length 16S rRNA gene sequencing by PacBio improves taxonomic resolution in human microbiome samples
BackgroundSequencing variable regions of the 16S rRNA gene (≃300 bp) with Illumina technology is commonly used to study the composition of human...
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Diversity, community structure, and quantity of eukaryotic phytoplankton revealed using 18S rRNA and plastid 16S rRNA genes and pigment markers: a case study of the Pearl River Estuary
Understanding consistencies and discrepancies in characterizing diversity and quantity of phytoplankton is essential for better modeling ecosystem...
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High-throughput identification and quantification of bacterial cells in the microbiota based on 16S rRNA sequencing with single-base accuracy using BarBIQ
Bacteria often function as a community, called the microbiota, consisting of many different bacterial species. The accurate identification of...
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An improved and extended dual-index multiplexed 16S rRNA sequencing for the Illumina HiSeq and MiSeq platform
BackgroundRecent advancements in next-generation sequencing (NGS) technology have ushered in significant improvements in sequencing speed and data...
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SituSeq: an offline protocol for rapid and remote Nanopore 16S rRNA amplicon sequence analysis
Microbiome analysis through 16S rRNA gene sequencing is a crucial tool for understanding the microbial ecology of any habitat or ecosystem. However,...
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A newly discovered bacterial symbiont in the aphid microbiome identified through 16S rRNA sequencing
Microbiome insect research has grown rapidly over the last years thanks to advances in next-generation sequencing. The bacterial microbiome...
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A detailed analysis of 16S rRNA gene sequencing and conventional PCR-based testing for the diagnosis of bacterial pathogens and discovery of novel bacteria
This study represents the first analysis of the bacterial community in chickens affected by swollen head syndrome, utilizing 16S rRNA gene...
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Bacterial Community Profile of Bovine Mastitis Infected Dairy Cow Milk by 16S rRNA Amplicon Sequencing
The prevalence of Bovine mastitis, an important disease of cow, causing huge economic loss in dairy industries, was tested in cows and other types of...
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16S rRNA metagenomic profiling of red amaranth grown organically with different composts and soils
AbstractIn recent years organic food is gaining popularity as it is believed to promote better human health and improve soil sustainability, but...
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Quantifying variation across 16S rRNA gene sequencing runs in human microbiome studies
AbstractRecent microbiome research has incorporated a higher number of samples through more participants in a study, longitudinal studies, and...
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A microbiome survey of contrasting potato terroirs using 16S rRNA long-read sequencing
AimsAs a consequence of the increasing impact of climate change on crop production and food security worldwide, the need to explore agricultural...
