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Development of B cell epitopes-based enzyme linked immune sorbent assay for detection of bovine anti-Mullerian hormone
The present study aimed to generate antibodies against predicted B cell epitopic peptides encoding bAMH for developing different ELISA models....
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Microfluidic 3D Cytotoxic Assay
Microfluidic-based cytotoxic assays provide high physiological relevance with the potential to replace conventional animal experiments and...
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A High-Throughput Single-Cell Assay on a Valve-Based Microfluidic Platform Applied to Protein Quantification, Immune Response Monitoring, and Drug Discovery
The use of microfluidic technology in single-cell assay has shown potential in biomedical applications like protein quantification, immune response...
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Direct Spectroscopic Ferrochelatase Assay
Ferrochelatases (E.C. 4.99.1.1) catalyze the insertion of ferrous iron into either protoporphyrin IX to make protoheme IX or coproporphyrin III to...
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Deep Learning-Based Automated Analysis of NK Cell Cytotoxicity in Single Cancer Cell Arrays
The cytotoxicity assay of immune cells based on live cell imaging offers comprehensive information at the single cell-level information, but the data...
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A fluorescence-based binding assay for proteins using the cell surface as a sensing platform
Protein–protein interaction (PPI) analysis is very important for elucidating the functions of proteins because many proteins execute their functions...
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Tackling assay interference associated with small molecules
Biochemical and cell-based assays are essential to discovering and optimizing efficacious and safe drugs, agrochemicals and cosmetics. However, false...
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Comparative Evaluation of Natural Killer Cell-mediated Cell Killing Assay Based on the Leakage of an Endogenous Enzyme or a Pre-loaded Fluorophore
A cell-killing ability of natural killer (NK) cells has been evaluated by the leakage of marker molecules from target cells. Lactate dehydrogenase...
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A Sample Preparation Procedure for Isobaric Labeling-Based Single-Cell Proteomics
Mass spectrometry-based proteomics has traditionally been limited by the amount of input material for analysis. Single-cell proteomics has emerged as...
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Mammalian Cell-Based Biosensors
The chapter deals with the start-of the art of mammalian cell-based biosensors given the main emphasize to optical, electrochemical, impedimetric...
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Ratiometric electrochemical OR gate assay for NSCLC-derived exosomes
Non-small cell lung cancer (NSCLC) is the most common pathological type of LC and ranks as the leading cause of cancer deaths. Circulating exosomes...
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Anti-cancer drug-mediated increase in mitochondrial mass limits the application of metabolic viability-based MTT assay in cytotoxicity screening
The high-throughput metabolic viability-based colorimetric MTT test is commonly employed to screen the cytotoxicity of different chemotherapeutic...
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Whole Cell Yeast-Based Biosensors
Analyte detection is a major component of fieldwork, environmental surveillance, and health protection, but lack of resources or access to...
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Detection of Norovirus Using Paper-Based Cell-Free Systems
Norovirus infections are the leading cause of foodborne illness and human gastroenteritis, afflicting hundreds of millions of people each year....
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Determination of L-selectin in blood plasma using DNA aptamer–based surface-enhanced Raman spectroscopy assay
In the human body, tumor cell occurrence can be indirectly monitored using the L-selectin concentration in the blood, since selectin ligands are...
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Towards a microfluidic H295R steroidogenesis assay—biocompatibility study and steroid detection on a thiol-ene-based chip
The development of cell-based microfluidic assays offers exciting new opportunities in toxicity testing, allowing for integration of new...
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A Microbead-Based Flow Cytometry Assay to Assess the Activity of Ubiquitin and Ubiquitin-Like Conjugating Enzymes
The peptidic posttranslational modifiers of the ubiquitin (Ub) family (ubiquitin-like, UbLs) are conjugated to thousands of proteins to modify their...
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Production of scaffold-free cell-based meat using cell sheet technology
In the production of cell-based meat, it is desirable to reduce animal-derived materials as much as possible to meet the challenges of...
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Measuring the Reversible Oxidation of Protein Tyrosine Phosphatases Using a Modified Cysteinyl-Labeling Assay
The modified cysteinyl-labeling assay enables the labeling, enrichment, and detection of all members of the protein tyrosine phosphatase (PTP)...
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Assay of Biomarkers for Alzheimer’s Disease by Surface Plasmon Resonance
Alzheimer’s disease (AD) is one of the neurodegenerative diseases characterized by cognitive dysfunction and behavioral impairment. Early diagnosis...
