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Assembly of TALEN and mTALE-Act for Plant Genome Engineering
Transcription activator-like effector (TALE) is a DNA-binding domain that can be paired with a nuclease to create DNA double-strand breaks, or with... -
Optimisation of a TALE nuclease targeting the HIV co-receptor CCR5 for clinical application
Disruption of the C-C-Chemokine-receptor-5 ( CCR5 ) gene induces resistance towards CCR5-tropic HIV. Here we optimised our previously described...
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The application of CRISPR/Cas9 system in cervical carcinogenesis
Integration of high-risk HPV genomes into cellular chromatin has been confirmed to promote cervical carcinogenesis, with HPV16 being the most...
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Efficient genome engineering of Toxoplasma gondii using the TALEN technique
BackgroundAromatic amino acid hydroxylase 2 (AAH2) is a bradyzoite-specific upregulated protein that may alter host behaviour by altering the host...
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Differentiation of endostyle cells by Nkx2-1 and FoxE in the ascidian Ciona intestinalis type A: insights into shared gene regulation in glandular- and thyroid-equivalent elements of the chordate endostyle
Due to similarities in iodine concentrations and peroxidase activities, the thyroid in vertebrates is considered to originate from the endostyle of...
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Innovative Approaches to Genome Editing in Chickens
Obtaining productive animals, including chickens, with specified characteristics is a promising area of modern animal husbandry. The most relevant...
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Targeted gene therapy into a safe harbor site in human hematopoietic progenitor cells
Directed gene therapy mediated by nucleases has become a new alternative to lead targeted integration of therapeutic genes in specific regions in the...
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Challenges and Future Prospects of CRISPR Technology
Genome editing (GE) tools have been revolutionizing life sciences by various marvelous applications for targeted gene modifications in organisms.... -
mRNA Transfection of T-Lymphocytes by Electroporation
Electroporation enables the transfection of different cell types including microbial, plant, and animal cells with charged molecules, such as nuclear... -
Protoplast-Based Method for Genome Editing in Tetraploid Potato
The cultivated potato is tetraploid with four probably equivalent loci for each gene. A potato variety is furthermore commonly genetically... -
Current advances in gene therapy of mitochondrial diseases
Mitochondrial diseases (MD) are a heterogeneous group of multisystem disorders involving metabolic errors. MD are characterized by extremely...
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CRISPR/Cas9 Mediated Therapeutic Approach in Huntington’s Disease
The pathogenic mechanisms of these diseases must be well understood for the treatment of neurological disorders such as Huntington's disease....
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Crop Breeding Genetic Improvement Methods
This volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for...
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Site-specific transgene integration in chimeric antigen receptor (CAR) T cell therapies
Chimeric antigen receptor (CAR) T cells and natural killer (NK) cells are genetically engineered immune cells that can detect target antigens on the...
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GMP-manufactured CRISPR/Cas9 technology as an advantageous tool to support cancer immunotherapy
BackgroundCRISPR/Cas9 system to treat human-related diseases has achieved significant results and, even if its potential application in cancer...
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Gene Editing in Human Haematopoietic Stem Cells for the Treatment of Primary Immunodeficiencies
In recent years, gene-editing technologies have revolutionised precision medicine, and human trials of this technology have been reported in...
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CRISPR/Cas9-mediated base editors and their prospects for mitochondrial genome engineering
Base editors are a type of double-stranded break (DSB)-free gene editing technology that has opened up new possibilities for precise manipulation of...
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hPSC gene editing for cardiac disease therapy
Cardiovascular diseases (CVDs) are the leading cause of mortality worldwide. However, the lack of human cardiomyocytes with proper genetic...
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DNA-Nukleasen und ihre Verwendung in der Viehzucht
DNA-Nukleasen, darunter Zink-Finger-Nukleasen (ZFN), Transkriptionsaktivator-ähnliche Endonukleasen (TALENS) und Meganukleasen, besitzen lange... -
Genome editing via non-viral delivery platforms: current progress in personalized cancer therapy
Cancer is a severe disease that substantially jeopardizes global health. Although considerable efforts have been made to discover effective...