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Identification of unique DNA methylation sites in Kabuki syndrome using whole genome bisulfite sequencing and targeted hybridization capture followed by enzymatic methylation sequencing
BackgroundKabuki syndrome (KS) is a congenital malformation syndrome caused by mutations in the KMT2D and KDM6A genes that encode histone...
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A capture methyl-seq protocol with improved efficiency and cost-effectiveness using pre-pooling and enzymatic conversion
ObjectiveThe opportunities for sequencing-based methylome analysis of clinical samples are increasing. To reduce its cost and the amount of genomic...
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Region Capture Micro-C reveals coalescence of enhancers and promoters into nested microcompartments
Although enhancers are central regulators of mammalian gene expression, the mechanisms underlying enhancer–promoter (E-P) interactions remain...
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Ancient and Modern Hybridization of Siberian Stone Pine and Dwarf Siberian Pine in the South of Yakutia
AbstractThe hybridization zone on the northern border of the overlapping ranges at the junction of the Prilensky Plateau with the Stanovoy and Aldan...
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Magnetic-bead-based DNA-capture-assisted real-time polymerase chain reaction and recombinase polymerase amplification for the detection of African swine fever virus
African swine fever (ASF) is a deadly disease in swine caused by African swine fever virus (ASFV). The global spread of ASFV has resulted in...
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Rabbit targeted genomic sequences after heterologous hybridization using human exome
ObjectiveCausal mutations for major genes that underlie a broad range of morphological traits are often located within exons of genes that then...
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Synaptic Tagging and Capture: Functional Implications and Molecular Mechanisms
This chapter introduces the concept of synaptic tagging and capture, which was first identified in electrophysiological studies in hippocampal brain...
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Hybrid capture shotgun sequencing detected unexpected viruses in the cerebrospinal fluid of children with acute meningitis and encephalitis
PurposeInvestigation of undiagnosed cases of infectious neurological diseases, especially in the paediatric population, remains a challenge. This...
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Genomic sequence capture of Plasmodium relictum in experimentally infected birds
BackgroundSequencing parasite genomes in the presence of host DNA is challenging. Sequence capture can overcome this problem by using RNA probes that...
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Hybridization is strongly constrained by salinity during secondary contact between silverside fishes (Odontesthes, Atheriniformes)
This study investigates a contact zone between two silverside fish species (marine Odontesthes argentinensis and freshwater O. bonariensis ) in the...
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Immunoglobulin/T Cell Receptor Capture Strategy for Comprehensive Immunogenetics
In the era of genomic medicine, targeted next generation sequencing strategies (NGS) are becoming increasingly adopted by clinical molecular...
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Microfluidics, CTC Capture, Analysis and Expansion
Cancer remains among the most common causes of morbidity and mortality worldwide. Although screening methods and better systemic treatment have led...
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Target capture sequencing reveals a monoclonal outbreak of respiratory syncytial virus B infections among adult hematologic patients
BackgroundRespiratory syncytial virus (RSV) causes community-acquired respiratory tract infections during winter. However, outbreaks in hospitals...
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Functional comparison of exome capture-based methods for transcriptomic profiling of formalin-fixed paraffin-embedded tumors
The availability of fresh frozen (FF) tissue is a barrier for implementing RNA sequencing (RNA-seq) in the clinic. The majority of clinical samples...
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Repurposing the Hybrid Capture 2 (HC2) screening test for whole-genome sequencing of human papillomaviruses
Simple and standardized approaches for genome analysis of human papillomavirus (HPV) by next-generation sequencing are needed. The aim of the study...
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Implementing a novel capture and ligation probe-PCR method in mass screen and treatment to support malaria elimination efforts in the China-Myanmar border region
BackgroundMass screening and treatment (MSAT) for malaria elimination lacks an ideal diagnostic tool to allow sensitive and affordable test of the...
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Quantitative and Selective DNA Detection with Portable Personal Glucose Meter Using Loop-Based DNA Competitive Hybridization Strategy
A portable, quantitative, and selective DNA detection biosensor, based on a loop-based DNA competitive hybridization assay and a personal glucose...
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HPV E6/E7 mRNA in situ hybridization in endocervical adenocarcinoma: implications for prognosis and diagnosis
BackgroundHuman papillomavirus (HPV) E6/E7 mRNA in situ hybridization (HPV E6/E7 RNAscope) appears to be a sensitive and specific technique in...
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Restriction enzyme selection dictates detection range sensitivity in chromatin conformation capture-based variant-to-gene mapping approaches
Promoter-focused chromatin conformation techniques directly detect interactions between gene promoters and distal genomic sequences, providing...
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Combined Fluorescent In Situ Hybridization (FISH) and Immunofluorescence for the Targeted Detection of Somatic Copy Number Variants in Synucleinopathies
There is increasing evidence for the role of mosaicism due to somatic mutations, including copy number variants (CNVs), in disorders of the nervous...
