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Evolution der PCR – von der klassischen PCR zur digitalen PCR
Keine Technik hat die Molekularbiologie so nachhaltig geprägt wie die Polymerasekettenreaktion (Polymerase Chain Reaction, PCR), und nach wie vor ist...
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Influence of physico-chemical properties of two lipoxin emulsion-loaded hydrogels on pre-polarized macrophages: a comparative analysis
Inflammation, a crucial defense mechanism, must be rigorously regulated to prevent the onset of chronic inflammation and subsequent tissue damage....
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Anti-rheumatoid arthritis potential of Halodule pinifolia: development, characterization and in vivo evaluation of H. pinifolia-based oral suspension and lipid nano-emulsion
For treating chronic diseases like rheumatoid arthritis, herbal medicines are preferred due to their evident therapeutic effects and lesser side...
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Minimal Residual Disease Analysis by Monitoring Immunoglobulin and T-Cell Receptor Gene Rearrangements by Quantitative PCR and Droplet Digital PCR
Analysis of immunoglobulin and T-cell receptor gene rearrangements by real-time quantitative polymerase chain reaction (RQ-PCR) is the gold standard...
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Droplet Digital PCR-Based Diagnosis for Porcine Viral Diseases
Polymerase chain reaction (PCR) is a common and indispensable technique that has been employed since its discovery for the diagnosis of infectious...
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Detection of Macrolide and/or Tetracycline Genetic Resistance Determinants by PCR
Globally, the main molecular trials being developed to study the genetic determinants responsible for conferring resistanceResistances to bacterial...
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Simplex Droplet Digital PCR Assays for the Detection of TERT Promoter Mutations in Urine Samples for the Non-invasive Diagnosis of Urothelial Cancer
Somatic mutations in the telomerase reverse transcriptase (TERT) promoter region are highly frequent in urothelial cancer (UC), and their detection...
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Polymerase Chain Reaction: An Indispensable Molecular Biology Tool of 21st Century
Due to its universal applications, Polymerase Chain Reaction (PCR) became an essential technique in various scientific branches such as microbiology,...
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Development of a multiplex droplet digital PCR assay for detection of enterovirus, parechovirus, herpes simplex virus 1 and 2 simultaneously for diagnosis of viral CNS infections
BackgroundEnterovirus (EV), parechovirus (HPeV), herpes simplex virus 1 and 2 (HSV1/2) are common viruses leading to viral central nervous system...
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Current and Emerging Applications of Droplet Digital PCR in Oncology: An Updated Review
In the era of personalized medicine and targeted therapies for the management of patients with cancer, ultrasensitive detection methods for tumor...
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Quantitation of Long Noncoding RNA Using Digital PCR
Long noncoding RNAs (lncRNAs) are implicated in many physiological or disease processes and alterations in their expression may contribute to the...
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Isolation and artificial production of atypical porcine pestivirus, using the swine-kidney-derived cell line SK-L
Congenital tremor (CT) in piglets was first reported in 1922, and although the causative pathogen was unknown for many years, atypical porcine...
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Development and Evaluation of a Droplet Digital PCR Assay for 8p23 β-Defensin Cluster Copy Number Determination
IntroductionIt has been proposed that the copy number (CN) variation (CNV) in β-defensin genes (DEFB) on human chromosome 8p23 determines phenotypic...
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Droplet-Based Microfluidic Chip Design, Fabrication, and Use for Ultrahigh-Throughput DNA Analysis and Quantification
DNA is widely used as a biomarker of contamination, infection, or disease, which has stimulated the development of a wide palette of detection and...
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Droplet digital PCR allows vector copy number assessment and monitoring of experimental CAR T cells in murine xenograft models or approved CD19 CAR T cell-treated patients
BackgroundGenetically engineered chimeric antigen receptor (CAR) T lymphocytes are promising therapeutic tools for cancer. Four CAR T cell drugs,...
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Development of a reverse transcription droplet digital PCR (RT-ddPCR) assay for sensitive detection of simian immunodeficiency virus (SIV)
BackgroundSimian immunodeficiency virus (SIV)-infected rhesus macaques constitute an excellent model of human HIV infection. Sensitive detection of...
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Rapid and Reliable Detection of Echinococcus multilocularis from Faeces Using Droplet Digital PCR
PurposeAlveolar echinococcosis is a severe helminthic disease in humans caused by larvae of the fox tapeworm Echinococcus multilocularis. Austria is...
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Measurement of gene amplifications related to drug resistance in Plasmodium falciparum using droplet digital PCR
BackgroundCopy number variations (CNVs) of the Plasmodium falciparum multidrug resistance 1 ( pfmdr1 ), P. falciparum plasmepsin2 ( pfplasmepsin2 ) and P....
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Enhancing Cohort PASA Efficiency from Lessons Assimilated by Mutant Genotyping in C. elegans
Classical restriction fragment length polymorphism (RFLP) and sequencing are labor-intensive and expensive methods to study single base changes,...
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Insights on Droplet Digital PCR–Based Cellular Kinetics and Biodistribution Assay Support for CAR-T Cell Therapy
Characterizing in vivo cellular kinetics and biodistribution of chimeric antigen receptor T (CAR-T) cells is critical for toxicity assessment,...
