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Nine-factor-based immunohistochemistry classifier predicts recurrence for early-stage hepatocellular carcinoma after curative resection
BackgroundImmunoscore have shown a promising prognostic value in many cancers. We aimed to establish and validate an immune classifier to predict...
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Pre- and Postembedding Immunoelectron Microscopy to Analyze the Central Nervous System
Immunocytochemistry, a method of delineating the subcellular localization of target proteins, was developed from immunohistochemistry. In principle,...
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Staining Hypoxic Areas of Frozen and FFPE Tissue Sections with Hypoxyprobe™
Hypoxia occurs due to inadequate levels of oxygen in tissue and has been implicated in numerous diseases such as cancer, diabetes, cardiovascular,...
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Tracking Cardiovascular Comorbidity in Models of Chronic Inflammatory Disease
Immune-mediated inflammatory diseases (IMIDs) are commonly associated with complex coexisting conditions, and cardiovascular comorbidities are a...
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Processing Neonatal Mouse Brains for Immunohistochemical Analysis: As Required for Including Spindle Orientation Analysis and Analysis of DNA Damage and Apoptosis
Analyzing sections of neonatal mouse brain using immunohistochemistry can inform microcephaly pathogenesis, but obtaining and staining high-quality...
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A Clinicopathological Approach to Odontogenic Cysts: the Role of Cytokeratin 17 and bcl2 Immunohistochemistry in Identifying Odontogenic Keratocysts
Odontogenic keratocysts (OKCs) are developmental cysts of the jaws that require proper diagnosis due to their potential for local aggressive growth...
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Novel Approaches to the Molecular Mapping of the Brain: 3D Cyclic Immunohistochemistry and Optical Clearing
This review considers the current methods required for functional mapping of neural networks in 3D space with cellular resolution. Cyclic...
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Preparation of Human Olfactory Epithelial Biopsies for Downstream Analysis
The olfactory mucosa, lining a portion of the nasal cavity, houses the primary olfactory sensory neurons responsible for odor transduction, along...
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Assessment of PD-L1 Status in Urothelial Cancer
Since 2016, the immunotherapeutic agents targeting PD-1 and PD-L1 are an integral part in first-line and second-line treatment of advanced or...
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Role of OCT4 in Pediatric Brain Tumor “Medulloblastoma” Development
Conventional H&E staining is still a gold standard for investigating the histopathology of solid tumors. Immunohistochemistry adds additional...
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Subcellular and Synaptic Distribution of Muscarinic Receptors in Neurons by Confocal and Electron Microscopy
The function of a G protein-coupled receptor in the modulation of neuronal activity is highly dependent on its availability at the cell surface, its...
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Droplet Digital PCR and Immunohistochemistry Techniques to Detect Zika Virus in the Central Nervous System of Mice
Detection of Zika virus (ZIKV) in the central nervous system (CNS) is a critical step when studying the pathogenesis of the infection in animal...
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The efficacy of DNA mismatch repair enzyme immunohistochemistry as a screening test for hypermutated gliomas
A subset of gliomas has DNA repair defects that lead to hypermutated genomes. While such tumors are resistant to alkylating chemotherapies, they may...
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Multiplex HPV RNA in situ hybridization/p16 immunohistochemistry: a novel approach to detect papillomavirus in HPV-related cancers. A novel multiplex ISH/IHC assay to detect HPV
BackgroundHigh-risk human papillomavirus (HR-HPV) is notoriously associated with tumor progression in a broad spectrum of malignancies. Detection of...
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Automated Immunofluorescence Staining for Analysis of DNA Damage and Apoptosis in Brain Sections
Neural progenitors show a strong tendency to undergo apoptosis in response to DNA damage, and both impaired DNA repair and increased neural...
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Quantitative Analysis of the Extracellular Matrix by Immunoblot
Knowing the global and local concentration of extracellular matrix proteins provides critical information for building tissue engineered constructs...
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Neuromuscular Assessments of Form and Function
This volume looks at the latest methods used to study imaging techniques, metabolic tracing, and deep muscle phenotyping. The chapters in this book...
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Histological Analysis of Trophoblast Cells in the Mouse Placental Labyrinth Zone
The mouse is a common animal species used for translational studies. In reproductive studies, this animal is typically preferred over other models as...
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Immunohistochemistry of advanced glycation end product Nε-(carboxymethyl)lysine in coronary arteries in relation to cardiac fibrosis and serum N-terminal-pro basic natriuretic peptide in forensic autopsy cases
ObjectiveAdvanced glycation end products (AGEs) are known to play important roles in the development of diabetes mellitus and atherosclerosis. N ε -(ca...
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Interrogating the Tumor Microenvironment by Deep Tissue Immunoprofiling
With the modern success of immunotherapy from chimeric antigen receptor T-cell therapy to checkpoint blockade, an understanding of the tumor...
