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Western Blot as a Support Technique for Immunohistochemistry to Detect Programmed Cell Death Ligand 1 Expression
Antibody selection and optimization are crucial to guarantee accurate and reproducible results when using such antibodies for applications such as...
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Creation of Formalin-Fixed, Paraffin-Embedded 3D Lung Cancer Cellular Spheroids for the Optimization of Immunohistochemistry Staining Procedures
In an era of precision medicine important treatment decisions are dictated by expression of clinically informative tumor protein biomarkers. These...
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Post-Embedding Immunohistochemistry in the Localization of Receptors and Ion Channels
Post-embedding techniques are powerful approaches for analysis of the chemical architecture of the brain in normal conditions and have proved useful...
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Detection of Mast Cells and Basophils by Immunohistochemistry
Staining cells or tissues with basic dyes was the mainstay of mast cell and basophil detection methods for more than a century following the first...
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Comparison of breast cancer surrogate subtyping using a closed-system RT-qPCR breast cancer assay and immunohistochemistry on 100 core needle biopsies with matching surgical specimens
BackgroundRoutine clinical management of breast cancer (BC) currently depends on surrogate subtypes according to estrogen- (ER) and progesterone (PR)...
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Diagnostic Accuracy of Immunohistochemistry for Mismatch Repair Proteins as Surrogate of Microsatellite Instability Molecular Testing in Endometrial Cancer
Microsatellite instability (MSI) defines one of the four molecular groups of endometrial carcinoma identified by The Cancer Genome Atlas (TCGA)....
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Immunohistochemistry for Protein Detection in Esophageal Squamous Cell Carcinoma
Immunohistochemistry is the identification of a cell protein by a specific antibody targeting that protein. It is the most common ancillary test to...
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Use of Antibodies in the Research on Muscarinic Receptor Subtypes
Antibodies can be a powerful tool to detect receptor expression at the protein level. Their main advantage is the potential of good spatial...
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Expression of stem cell markers as useful complementary factors in the early detection of urinary bladder carcinogens by immunohistochemistry for γ-H2AX
We previously demonstrated that immunohistochemistry for γ-H2AX, a biomarker of DNA damage, is useful for early detection of urinary bladder...
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Histopathological Analysis of Brains from Dogs Infected with Canine Distemper Virus
We describe the use of conventional histology and immunohistochemistry against canine distemper virus (CDV) to examine the brains of domestic dogs...
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Prevalence of Helicobacter pylori among Sudanese patients diagnosed with colon polyps and colon cancer using immunohistochemistry technique
ObjectivesInfection with the bacteria Helicobacter pylori has been classified as class one carcinogen associated with increasing susceptibility of...
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Retrospective observational study of HER2 immunohistochemistry in borderline breast cancer patients undergoing neoadjuvant therapy, with an emphasis on Group 2 (HER2/CEP17 ratio ≥2.0, HER2 copy number <4.0 signals/cell) cases
BackgroundThe ASCO/CAP guidance on HER2 testing in breast cancer (BC) has recently changed. Group 2 tumours with immunohistochemistry score 2+ and HER2...
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Interleukin-1 (IL-1) Immunohistochemistry Assay in Oral Squamous Cell Carcinoma
This section describes an immunohistochemistry method to analyze interleukin-1 (IL-1) in oral squamous cell carcinoma. The described protocol has...
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Specific Detection of Neu5Gc in Animal Tissues by Immunohistochemistry
One of the major obstacles in xenotransplantation is tissue expression of the non-human mammalian carbohydrate N-glycolylneuraminic acid (Neu5Gc)....
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Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques
In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and...
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Isolation and Detection of Pathological Tau Species in a Tauopathy Mouse Model
Tau protein in Alzheimer’s disease (AD) and tauopathies becomes insoluble due to hyperphosphorylation, conformational alterations, and aggregation....
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Immunohistochemistry for Tumor-Infiltrating Immune Cells After Oncolytic Virotherapy
Immunohistochemistry (IHC) is an integral laboratory staining technique, which is used for the detection of immune cells in mouse/human tissues or...
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Immunohistochemistry-based assessment of androgen receptor status and the AR-null phenotype in metastatic castrate resistant prostate cancer
BackgroundMolecular and immunohistochemistry-based profiling of prostatic adenocarcinoma has revealed frequent Androgen Receptor ( AR ) gene and...
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Spatial Technologies: A Game Changer for Studying the Tumor Microenvironment
Traditional immunohistochemistry (IHC) has long been considered the “gold standard” for tissue pathology diagnosis. However, the diagnostic and...
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Detection of West Nile Virus Envelope Protein in Brain Tissue with an Immunohistochemical Assay
Immunohistochemistry is a valuable tool for probing not only scientific questions but also clinical diagnoses. It provides power from localization of...
