Abstract
Endophytic actinomycetes from five Asteraceae plants were isolated and evaluated for their bioactivities. From Parthenium hysterophorus, Ageratum conyzoides, Sonchus oleraceus, Sonchus asper and Hieracium canadense, 42, 45, 90, 3, and 2 isolates, respectively, were obtained. Of the isolates, 86 (47.2 %) showed antimicrobial activity. Majority of the isolates were recovered from the roots (n = 127, 69.7 %). The dominant genus was Streptomyces (n = 96, 52.7 %), while Amycolatopsis, Pseudonocardia, Nocardia and Micromonospora were also recovered. Overall, 36 of the 86 isolates were significantly bioactivity while 18 (20.9 %) showed strong bioactivity. In total, 52.1 and 66.6 % showed potent cytotoxicity and antioxidant activities. The LC50 for 15 strains was <20 μg/ml. Compared to the ascorbate standard (EC50 0.34 μg/ml), all isolates gave impressive results with notable EC50 values of 0.65, 0.67, 0.74 and 0.79 μg/ml.
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Acknowledgments
The authors thank the late Prof. Dr. Ghazala Nasim of the Institute of Plant Pathology and Dr. Sikandar Sultan at the Department of Microbiology and Molecular Genetics, University of the Punjab for the help with the identification of the plants.
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Cytotoxicity test using extracts of Asteraceae plant endophytic actinomycetes against Artemia salina nauplii (JPEG 88 kb)
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Tanvir, R., Sajid, I. & Hasnain, S. Biotechnological potential of endophytic actinomycetes associated with Asteraceae plants: isolation, biodiversity and bioactivities. Biotechnol Lett 36, 767–773 (2014). https://doi.org/10.1007/s10529-013-1430-0
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DOI: https://doi.org/10.1007/s10529-013-1430-0