Ammonia attracts the haematophagous bug Triatoma infestans : behavioural and neurophysiological data on nymphs

1) Nymphs of the haematophagous bug Triatoma infestans (Heteroptera: Reduviidae) are attracted to volatiles from their own faeces on a servosphere. 2) Biological substrates attractive to triatomines release NH₃: wetted triatomine faecal papers release NH₃ at 256 ppb NH₃ from a 60-g source and stale rabbit urine at 394 ppb from 200 ml. Ammonia released from aqueous NH₃ also attracts bugs at doses of 3 ppb and 17 ppb on the servosphere. 3) Bugs typically show negative anemotaxis in a stimulus-free air-stream on the servosphere. At onset of stimulation with ammonia from either biological substrates or aqueous NH₃ the bugs stop, move their antennae, turn and walk upwind, i.e. odour-mediated anemotaxis. 4) At lower NH₃ doses a latency in attraction is recorded, but this latency disappears when the relative humidity of the stimulus delivery air-stream is dropped from 90 to 35%. 5) Electrophysiological recordings from single olfactory sensilla on antennae of Triatoma nymphs reveal two different types of NH₃-excited receptors, both within grooved-peg sensilla. The responses of one of these receptor cells to NH₃ has been studied in detail and shows that the action potential discharge rate is dose-dependent over the range 2–200 ppb. 6) The amplitudes of electroantennograms recorded from Triatoma nymphs to NH₃ are dose dependent over the range 5–550 ppb.

Authors and Affiliations

1. Institute of Zoology, University of Neuchâtel, Rue Emile-Argand 11, CH-2007 Neuchâtel, Switzerland, Tel.: +41-32/7183066, Fax: +41-32/7183001, , , , , , CH

   J. Taneja & P. M. Guerin

Accepted: 16 January 1997

Reprints and permissions