Summary
Ribosomal RNAs of 17 species of archaebacteria were hybridized to corresponding and non-corresponding nitrocellulose bound DNAs. The temperature stability of these hybrids and the amount of bound rRNA were determined.
A formula was derived to correct the hybridization yields for the different genome lengths and numbers of rRNA operons per genome. This correction made it possible to determine hybridization homologies, as functions of velocity constants, which could then be used in a similar way as sequence homologies.
The results were consistent with those from 16S rRNA total sequence data. No correlation was found between the hybridization homologies and the temperature stabilities of the hybrids.
This new method is faster and simpler than the method based on total 16S rRNA sequence determination although it provides less total information. Its application to archaebacterial phylogeny has shown theThermococcales to represent a third branch of the kingdom beside the branch of the methanogens + halophiles, and that of theThermoproteales + Sulfolobales. The method has also provided a detailed description of the phylogeny of theSulfolobales showing their origin within theThermoproteales.
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Klenk, H.P., Haas, B., Schwass, V. et al. Hybridization homology: A new parameter for the analysis of phylogenetic relations, demonstrated with the urkingdom of the archaebacteria. J Mol Evol 24, 167–173 (1986). https://doi.org/10.1007/BF02099964
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DOI: https://doi.org/10.1007/BF02099964