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Detection and Elimination of Cellular Bottlenecks in Protein-Producing Yeasts

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Recombinant Protein Production in Yeast

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1923))

Abstract

Yeasts are efficient cell factories and are commonly used for the production of recombinant proteins for biopharmaceutical and industrial purposes. For such products high levels of correctly folded proteins are needed, which sometimes requires improvement and engineering of the expression system. The article summarizes major breakthroughs that led to the efficient use of yeasts as production platforms and reviews bottlenecks occurring during protein production. Special focus is given to the metabolic impact of protein production. Furthermore, strategies that were shown to enhance secretion of recombinant proteins in different yeast species are presented.

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Acknowledgments

Work in the group of Pau Ferrer has been supported by the project CTQ2016-74959-R (AEI/FEDER, UE) of the Spanish Ministry of Economy, Industry and Competitiveness (MINECO), and the Catalan Government (Research Group 2017-SGR-1462 and Xarxa de Referència en Biotecnologia).

Research on yeast protein production at BOKU is supported by the Austrian Science Fund (FWF): Doctoral Program BioToP—Biomolecular Technology of Proteins (FWF W1224), the Austrian Federal Ministry for Digital and Economic Affairs (BMDW), the Federal Ministry of Traffic, Innovation and Technology (bmvit), the Styrian Business Promotion Agency SFG, the Standortagentur Tirol, the Government of Lower Austria and ZIT—Technology Agency of the City of Vienna through the COMET-Funding Program managed by the Austrian Research Promotion Agency FFG (RZ, BG, DM), and by the Christian Doppler Research Association, the Austrian Federal Ministry for Digital and Economic Affairs (BMDW) and the National Foundation for Research, Technology and Development (BG).

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Authors and Affiliations

  1. Department of Biotechnology, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria

    Richard J. Zahrl

  2. Austrian Centre of Industrial Biotechnology (acib), Vienna, Austria

    Richard J. Zahrl

  3. Christian Doppler-Laboratory for Growth-Decoupled Protein Production in Yeast, Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria

    Brigitte Gasser

  4. Luxembourg Institute of Science and Technology, Belvaux, Luxembourg

    Pau Ferrer

  5. Department of Chemical, Biological and Environmental Engineering, Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), Catalonia, Spain

    Pau Ferrer

  6. Department of Biotechnology, University of Natural Resources and Life Sciences (BOKU) and Austrian Centre of Industrial Biotechnology (acib), Vienna, Austria

    Brigitte Gasser & Diethard Mattanovich

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  1. Richard J. Zahrl
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  2. Brigitte Gasser
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  3. Diethard Mattanovich
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  4. Pau Ferrer
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Correspondence to Pau Ferrer .

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Editors and Affiliations

  1. Department of Biotechnology, University of Natural Resources and Life Sciences (BOKU) and Austrian Centre of Industrial Biotechnology (acib), Vienna, Austria

    Brigitte Gasser

  2. Department of Biotechnology, University of Natural Resources and Life Sciences (BOKU) and Austrian Centre of Industrial Biotechnology (acib), Vienna, Austria

    Diethard Mattanovich

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Zahrl, R.J., Gasser, B., Mattanovich, D., Ferrer, P. (2019). Detection and Elimination of Cellular Bottlenecks in Protein-Producing Yeasts. In: Gasser, B., Mattanovich, D. (eds) Recombinant Protein Production in Yeast. Methods in Molecular Biology, vol 1923. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9024-5_2

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