Abstract
The availability of cloned mycobacterial genes and suitable vectors for expression in mammalian cells has now opened a new avenue in which individual mycobacterial protein antigens can be tested for their ability to confer protective immunity. Expression of protection against mycobacteria is largely a function of antigen specific CD4 and cytotoxic CD8 T cells. We have found that intramuscular injection of plasmid DNA expressing several mycobacterial immunodominant genes can induce both T cell populations and confer protection equivalent to BCG, suggesting that this approach may lead to a new vaccine.
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Barry M. A., Lai W. C., Johnston S.A., 1995, Protection against mycoplasma infection using expression library immunization. Nature, 377: 632.
Freeman, G. J., Gray, G. S., Gimmi, C. D., Lombard, D. B., Zou, L. White, M., Fingeroth, J. D., Gribben, J. G., and Nadler, L. M., 1991, Structure, expression, and T cell costimulatory activity of the murine homologue of the human B lymphocyte activation antigen B7. J. Exp. Med., 174: 625.
Gautier, C., Mehtali, M. and Lathe, R., 1989, A ubiquitous mammalian expression vector, pHMG based on a housekeeping gene promoter. Nucleic Acids Res., 17: 8389.
Matzinger P., 1991, The JAM test a simple assay for DNA fragmentation and cell death. J.Immunol.Methods., 145: 185.
Silva, C.L. and Lowrie, D.B., 1994, A single mycobacterial protein (hsp65) expressed by a transgenic antigen-resenting cell vaccinates mice against tuberculosis. Immunol., 82: 244.
Silva, C.L., Silva, M.F., Pietro, R.C.L.R. and Lowrie, D.B. 1994, Protection against tuberculosis by passive transfer with T-cell clones recognizing mycobacterial heat-shock protein 65. Immunol., 83: 341.
Sorensen, A.L., Nagai, S., Houen, G., Andersen, P. and Andersen, A.B., 1995, Purification and characterization of a low-molecular-mass T-cell antigen secreted by Mycobacterium tuberculosis. Infect. Immun., 63: 1710.
Tascon, R.E., Colston, M. J., Ragno S., Stavropoulos, E., Gregory D., Lowrie D.B., 1996, Vaccination against tuberculosis by DNA injection. Nature Medicine, 2: 888.
Taswell, C., 1981, Limiting dilution assays for the determination of immunocompetent cell frequencies. I. Data analysis. J. Immunol., 126: 1614.
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© 1997 Springer Science+Business Media New York
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Tascon, R.E., Colston, M.J., Stavropoulos, E., Ragno, S., Gregory, D., Lowrie, D.B. (1997). Protection Against Tuberculosis by Plasmid DNA. In: Gregoriadis, G., McCormack, B., Allison, A.C. (eds) Vaccine Design. NATO ASI Series, vol 293. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0062-3_18
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DOI: https://doi.org/10.1007/978-1-4899-0062-3_18
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