Abstract
The automated system presently are in their budding phase in diagnosis of fungal disease as most of them providing identification of yeast along with their antifungal sensitivity pattern. Very few equipments have been approved for identification of filamentous fungi. Further, due to high cost, their availability are still limited in high end diagnostic laboratory especially in developing countries. Nevertheless, their features of automated, faster, precise, sensitive and more accurate identification with minimum cross reactivity as well as antimicrobial susceptibility adequately may compensate the initial higher cost of the equipment. The rapid identification and antimicrobial sensitivity report may immensely help clinician in initiating early evidence based precise treatment to reduce mortality especially in invasive fungal diseases by reducing treatment cost and hospital stay to ultimately help the patients, their family and the country with lesser disease and economic burden. These features are likely to justify their requirement in diagnostic microbiology including mycology as the integral part of the laboratory for early, precise and accurate diagnosis of fungal diseases.
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References
Becker PT, et al. Identification of filamentous fungi isolates by MALDI-TOF mass spectrometry: clinical evaluation of an extended reference spectra library. Med Mycol. 2014;52(8):826–34. https://doi.org/10.1093/mmy/myu064.
Peng Y, Zhang Q, Xu C, Shi W. MALDI-TOF MS for the rapid identification and drug susceptibility testing of filamentous fungi. Exp Ther Med. 2019;18(6):4865–73. https://doi.org/10.3892/etm.2019.8118.
Motteu N, Goemaere B, Bladt S, Packeu A. Implementation of MALDI-TOF mass spectrometry to identify fungi from the indoor environment as an added value to the classical morphology-based identification tool. Front Allergy. 2022;3:826148. https://doi.org/10.3389/falgy.2022.826148.
Wadlin JK, Hanko G, Stewart R, Pape J, Nachamkin I. Comparison of three commercial systems for identification of yeasts commonly isolated in the clinical microbiology laboratory. J Clin Microbiol. 1999;37(6):1967–70. https://doi.org/10.1128/JCM.37.6.1967-1970.1999.
Lacroix C, et al. Evaluation of two matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) systems for the identification of Candida species. Clin Microbiol Infect. 2014;20(2):153–8. https://doi.org/10.1111/1469-0691.12210.
Hussain KK, et al. Biosensors and diagnostics for fungal detection. J Fungi (Basel, Switzerland). 2020;6(4):349. https://doi.org/10.3390/jof6040349.
Lau AF, et al. Multicenter study demonstrates standardization requirements for Mold identification by MALDI-TOF MS. Front Microbiol. 2019;10:2098. https://doi.org/10.3389/fmicb.2019.02098.
Reslova N, Michna V, Kasny M, Mikel P, Kralik P. xMAP technology: applications in detection of pathogens. Front Microbiol. 2017;8:55. https://doi.org/10.3389/fmicb.2017.00055.
Hata DJ, Hall L, Fothergill AW, Larone DH, Wengenack NL. Multicenter evaluation of the new VITEK 2 advanced colorimetric yeast identification card. J Clin Microbiol. 2007;45(4):1087–92. https://doi.org/10.1128/JCM.01754-06.
Pfaller MA, Diekema DJ, Procop GW, Rinaldi MG. Multicenter comparison of the VITEK 2 antifungal susceptibility test with the CLSI broth microdilution reference method for testing amphotericin B, flucytosine, and voriconazole against Candida spp. J Clin Microbiol. 2007;45(11):3522–8. https://doi.org/10.1128/JCM.00403-07.
CDC. “Identification of Candida auris.” https://www.cdc.gov/fungal/Candida-auris/identification.html. 2020. https://www.cdc.gov/fungal/Candida-auris/identification.html. Accessed 28 Nov 2022.
Hamal P, Vavrova A, Mrazek J, Svobodova L. Identification of filamentous fungi including dermatophytes using MALDI-TOF mass spectrometry. Folia Microbiol (Praha). 2022;67(1):55–61. https://doi.org/10.1007/s12223-021-00917-6.
Florio W, Tavanti A, Ghelardi E, Lupetti A. MALDI-TOF MS applications to the detection of antifungal resistance: state of the art and future perspectives. Front Microbiol. 2018;9:2577. https://doi.org/10.3389/fmicb.2018.02577.
Vatanshenassan M, et al. Proof of concept for MBT ASTRA, a rapid matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based method to detect Caspofungin resistance in Candida albicans and Candida glabrata. J Clin Microbiol. 2018;56(9):1. https://doi.org/10.1128/JCM.00420-18.
Zacharioudakis IM, Zervou FN, Mylonakis E. T2 magnetic resonance assay: overview of available data and clinical implications. J Fungi (Basel, Switzerland). 2018;4(2):45. https://doi.org/10.3390/jof4020045.
Neely LA, et al. T2 magnetic resonance enables nanoparticle-mediated rapid detection of candidemia in whole blood. Sci Transl Med. 2013;5(182):182ra54. https://doi.org/10.1126/scitranslmed.3005377.
Morrell M, Fraser VJ, Kollef MH. Delaying the empiric treatment of candida bloodstream infection until positive blood culture results are obtained: a potential risk factor for hospital mortality. Antimicrob Agents Chemother. 2005;49(9):3640–5. https://doi.org/10.1128/AAC.49.9.3640-3645.2005.
Sexton DJ, Bentz ML, Welsh RM, Litvintseva AP. Evaluation of a new T2 magnetic resonance assay for rapid detection of emergent fungal pathogen Candida auris on clinical skin swab samples. Mycoses. 2018;61(10):786–90. https://doi.org/10.1111/myc.12817.
Leach L, Russell A, Zhu Y, Chaturvedi S, Chaturvedi V. A rapid and automated sample-to-result Candida auris real-time PCR assay for high-throughput testing of surveillance samples with the BD max open system. J Clin Microbiol. 2019;57(10):10. https://doi.org/10.1128/JCM.00630-19.
Lima A, Widen R, Vestal G, Uy D, Silbert S. A TaqMan probe-based real-time PCR assay for the rapid identification of the emerging multidrug-resistant pathogen Candida auris on the BD max system. J Clin Microbiol. 2019;57(7):10–128. https://doi.org/10.1128/JCM.01604-18.
Chien J-Y, et al. Evaluation of the automated Becton Dickinson MAX real-time PCR platform for detection of pneumocystis jirovecii. Future Microbiol. 2017;12:29–37. https://doi.org/10.2217/fmb-2016-0115.
Marucco AP, Minervini P, Snitman GV, Sorge A, Guelfand LI, Moral LL. Comparison of the identification results of Candida species obtained by BD Phoenix™ and Maldi-TOF (Bruker microflex LT Biotyper 3.1). Rev Argent Microbiol. 2018;50(4):337–40. https://doi.org/10.1016/j.ram.2017.10.003.
Posteraro B, et al. Comparative evaluation of BD Phoenix and vitek 2 systems for species identification of common and uncommon pathogenic yeasts. J Clin Microbiol. 2013;51(11):3841–5. https://doi.org/10.1128/JCM.01581-13.
Ceballos-Garzón A, et al. Comparison between MALDI-TOF MS and MicroScan in the identification of emerging and multidrug resistant yeasts in a fourth-level hospital in Bogotá, Colombia. BMC Microbiol. 2019;19(1):106. https://doi.org/10.1186/s12866-019-1482-y.
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Negi, S.S. (2024). Automation Technique in Medical Mycology. In: Kumar, S., Kumar, A. (eds) Automated Diagnostic Techniques in Medical Microbiology. Springer, Singapore. https://doi.org/10.1007/978-981-99-9943-9_6
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