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Reverse Genetics of Dengue Virus

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Reverse Genetics of RNA Viruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2733))

Abstract

Dengue virus (DENV) is one of the most important and widespread arthropod-borne viruses, causing millions of infections over the years. Considering its epidemiological importance, efforts have been directed towards understanding various aspects of DENV biology, which have been facilitated by the development of different molecular strategies for engineering viral genomes, such as reverse genetics approaches. Reverse genetic systems are a powerful tool for investigating virus–host interaction, for vaccine development, and for high-throughput screening of antiviral compounds. However, stable manipulation of DENV genomes is a major molecular challenge, especially when using conventional cloning systems. To circumvent this issue, we describe a simple and efficient yeast-based reverse genetics system to recover infectious DENV clones.

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Author information

Authors and Affiliations

  1. Virology Sector, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, RS, Brazil

    José Valter Joaquim Silva Júnior

  2. Virology Sector, Laboratory of Immunopathology Keizo Asami, Federal University of Pernambuco, Recife, PE, Brazil

    José Valter Joaquim Silva Júnior

  3. Virology Laboratory, Institute of Biological Sciences, Federal University of Pará, Belém, PA, Brazil

    Andréa Nazaré Monteiro Rangel da Silva

  4. Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA

    Jefferson José da Silva Santos

  5. Laboratory of Virology and Experimental Therapy, Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, PE, Brazil

    Laura Helena Vega Gonzales Gil

Authors
  1. José Valter Joaquim Silva Júnior
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  2. Andréa Nazaré Monteiro Rangel da Silva
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  3. Jefferson José da Silva Santos
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  4. Laura Helena Vega Gonzales Gil
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Corresponding author

Correspondence to Laura Helena Vega Gonzales Gil .

Editor information

Editors and Affiliations

  1. College of Veterinary Medicine, University of Georgia, Athens, GA, USA

    Daniel R. Pérez

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Silva Júnior, J.V.J., da Silva, A.N.M.R., da Silva Santos, J.J., Gil, L.H.V.G. (2024). Reverse Genetics of Dengue Virus. In: Pérez, D.R. (eds) Reverse Genetics of RNA Viruses. Methods in Molecular Biology, vol 2733. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3533-9_14

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  • DOI: https://doi.org/10.1007/978-1-0716-3533-9_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3532-2

  • Online ISBN: 978-1-0716-3533-9

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