Abstract
Testis-resident macrophages are first responders of the innate immune system against pathogens. They also exert day-to-day functions that are poorly understood. To study testis macrophages, several techniques are used, among which we can find flow cytometry.
Flow cytometry is a powerful tool that enables analysis of macrophages at a cellular as well as population level. To analyze testis macrophages using flow cytometry, a specific tissue processing is necessary to extract them. In this protocol, we explain how to extract and analyze the distinct macrophage populations.
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References
Hume DA, Irvine KM, Pridans C (2019) The mononuclear phagocyte system: the relationship between monocytes and macrophages. Trends Immunol 40(2):98–112. https://doi.org/10.1016/j.it.2018.11.007
Sheu KM, Hoffmann A (2022) Functional hallmarks of healthy macrophage responses: their regulatory basis and disease relevance. Annu Rev Immunol 40:295–321. https://doi.org/10.1146/annurev-immunol-101320-031555
Nobs SP, Kopf M (2021) Tissue-resident macrophages: guardians of organ homeostasis. Trends Immunol 42(6):495–507. https://doi.org/10.1016/j.it.2021.04.007
Wang Y, Chaffee TS, LaRue RS, Huggins DN, Witschen PM, Ibrahim AM, Nelson AC, Machado HL, Schwertfeger KL (2020) Tissue-resident macrophages promote extracellular matrix homeostasis in the mammary gland stroma of nulliparous mice. elife 9. https://doi.org/10.7554/eLife.57438
Bellomo A, Mondor I, Spinelli L, Lagueyrie M, Stewart BJ, Brouilly N, Malissen B, Clatworthy MR, Bajenoff M (2020) Reticular fibroblasts expressing the transcription factor WT1 define a stromal niche that maintains and replenishes splenic red pulp macrophages. Immunity 53(1):127–142. e127. https://doi.org/10.1016/j.immuni.2020.06.008
Lokka E, Lintukorpi L, Cisneros-Montalvo S, Makela JA, Tyystjarvi S, Ojasalo V, Gerke H, Toppari J, Rantakari P, Salmi M (2020) Generation, localization and functions of macrophages during the development of testis. Nat Commun 11(1):4375. https://doi.org/10.1038/s41467-020-18206-0
McKinnon KM (2018) Flow cytometry: an overview. Curr Protoc Immunol 120:511–5111. https://doi.org/10.1002/cpim.40
Szaloki G, Goda K (2015) Compensation in multicolor flow cytometry. Cytometry A 87(11):982–985. https://doi.org/10.1002/cyto.a.22736
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Sekias, M., Baratin, M., Bajenoff, M. (2024). Isolation and Characterization of Testis Macrophages Using Flow Cytometry. In: Mass, E. (eds) Tissue-Resident Macrophages. Methods in Molecular Biology, vol 2713. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3437-0_14
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DOI: https://doi.org/10.1007/978-1-0716-3437-0_14
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3436-3
Online ISBN: 978-1-0716-3437-0
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