Enzymatic Immunohistochemistry

Immunohistochemistry (IHC) is a powerful laboratory technique that is widely used in a variety of settings, including clinical medicine and research. Enzymatic immunohistochemistry refers to the process of detecting cell surface proteins or antigens using enzyme-labeled antibodies. The site and specificity of the antibody binding may be identified using direct labeling techniques or through secondary labeling methods. The antibody-antigen interaction is ultimately visualized using a chromogenic detection process, in which the enzyme conjugated to the antibody cleaves a substrate to produce a stable, colored precipitate.

Authors and Affiliations

1. Department of Pathology, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR, USA

   Rodolfo Henrich Lobo

Corresponding author

Correspondence to Rodolfo Henrich Lobo .

Editors and Affiliations

1. Department of Pathology and Medicine & Louisiana Cancer Research Center, Louisiana State University Health Sciences Center, New Orleans, LA, USA

   Luis Del Valle

Reprints and permissions

© 2022 Springer Science+Business Media, LLC, part of Springer Nature

Policies and ethics