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Cancer Stem Cells

Methods and Protocols

  • Book
  • © 2024
  • Latest edition

Overview

Editors:
  1. Federica Papaccio

    1. Department of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, Baronissi, Italy

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  2. Gianpaolo Papaccio

    1. Department of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples, Italy

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2777)

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About this book

This detailed new edition gathers a comprehensive collection of methods, protocols, and procedures used for the identification, characterization, and selection of cancer stem cells. New chapters focus on the latest technologies that have improved our knowledge in this field, such as organoids, machine learning, nanoparticles, and other recent advances. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Cancer Stem Cells: Methods and Protocols, Second Edition provides researchers with the key techniques needed to increase our understanding of this important field of study.

Keywords

Table of contents (17 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Cancer Stem Cells: Current Challenges and Future Perspectives

    • Muhammad Vaseem Shaikh, Stefan Custers, Alisha Anand, Petar Miletic, Chitra Venugopal, Sheila K. Singh
    Pages 1-18

  3. Immunohistochemistry for Cancer Stem Cell Detection: Principles and Methods

    • Giuseppa Zannini, Renato Franco, Federica Zito Marino
    Pages 19-33

  4. Isolating Cancer Stem Cells from Solid Tumors

    • Vitale Del Vecchio, Marcella La Noce, Virginia Tirino
    Pages 35-49

  5. Surface Markers for the Identification of Cancer Stem Cells

    • Tasfik Ul Haque Pronoy, Farhadul Islam, Vinod Gopalan, Alfred King-yin Lam
    Pages 51-69

  6. CD44-Based Detection of CSCs: CD44 Immunodetection by Flow Cytometry

    • Lornella Seeneevassen, Anissa Zaafour, Christine Varon
    Pages 71-81

  7. ALDH Activity Assay: A Method for Cancer Stem Cell (CSC) Identification and Isolation

    • Vitale Del Vecchio, Marcella La Noce, Virginia Tirino
    Pages 83-89

  8. In Vitro Tumorigenic Assay: A Tumor Sphere Assay for Cancer Stem Cells

    • Amani Yehya, Hisham Bahmad, Wassim Abou-Kheir
    Pages 91-98

  9. Multicellular Tumoroids for Investigating Cancer Stem-Like Cells in the Heterogeneous Tumor Microenvironment

    • Kathleen M. Burkhard, Geeta Mehta
    Pages 99-122

  10. Generation, Expansion, and Biobanking of Gastrointestinal Patient-Derived Organoids from Tumor and Normal Tissues

    • Manuel Cabeza-Segura, Blanca Garcia-Micó, Andrés Cervantes, Josefa Castillo
    Pages 123-133

  11. Prostate Cancer Organoids for Tumor Modeling and Drug Screening

    • Amani Yehya, Fatima Ghamlouche, Sana Hachem, Wassim Abou-Kheir
    Pages 135-144

  12. Mimicking the Tumor Niche: Methods for Isolation, Culture, and Characterization of Cancer Stem Cells and Multicellular Spheroids

    • Laura De Lara-Peña, Cristiano Farace, Andrea Pisano, Julia López de Andrés, Grazia Fenu, Federica Etzi et al.
    Pages 145-161

  13. Detection of Cancer Stem Cells in Normal and Dysplastic/Leukemic Human Blood

    • Alessia De Stefano, Alessandra Cappellini, Irene Casalin, Stefania Paolini, Sarah Parisi, Maria Vittoria Marvi et al.
    Pages 163-176

  14. Methods to Study the Role of Mechanical Signals in the Induction of Cancer Stem Cells

    • Alessandro Gandin, Paolo Contessotto, Tito Panciera
    Pages 177-189

  15. Co-Delivery Polymeric Poly(Lactic-Co-Glycolic Acid) (PLGA) Nanoparticles to Target Cancer Stem-Like Cells

    • Catherine S. Snyder, Taylor Repetto, Kathleen M. Burkhard, Anish Tuteja, Geeta Mehta
    Pages 191-204

  16. Isolating Circulating Cancer Stem Cells (CCSCs) from Human Whole Blood

    • Carla Kantara, Pomila Singh
    Pages 205-218

  17. Generation of Cancer Stem Cells by Co-Culture Methods

    • Biswajit Das, Chanakya Nath Kundu
    Pages 219-230

  18. Deep Learning of Cancer Stem Cell Morphology

    • Hiroyuki Kameda, Hiroaki Ishihata, Tomoyasu Sugiyama
    Pages 231-256

  19. Back Matter

    Pages 257-259
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Editors and Affiliations

  • Department of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, Baronissi, Italy

    Federica Papaccio

  • Department of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples, Italy

    Gianpaolo Papaccio

Bibliographic Information

  • Book Title: Cancer Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Federica Papaccio, Gianpaolo Papaccio

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3730-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3729-6Published: 14 March 2024

  • Softcover ISBN: 978-1-0716-3732-6Due: 14 April 2024

  • eBook ISBN: 978-1-0716-3730-2Published: 13 March 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 259

  • Number of Illustrations: 13 b/w illustrations, 47 illustrations in colour

  • Topics: Cancer Research, Cancer Research

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