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Germ Cell Development

Methods and Protocols

  • Book
  • © 2024

Overview

Editors:
  1. Marco Barchi

    1. Department of Biomedicine and Prevention, Section of Human Anatomy, Faculty of Medicine and Surgery, “Tor Vergata“ University of Rome, Rome, Italy

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  2. Massimo De Felici

    1. Department of Biomedicine and Prevention, Section of Histology and Embryology, Faculty of Medicine and Surgery, “Tor Vergata” University of Rome, Rome, Italy

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2770)

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About this book

This detailed volume explores techniques for the isolation, purification, and establishment of in vitro germ cell (GS) systems at different stages of development in a variety of organisms, including chickens, mice, rats, and humans. The book additionally describes cutting-edge analytic and informatic tools to study germ cell development at the single cell level and meiotic recombination. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Germ Cell Development: Methods and Protocols provides new insight into the understanding of basic biological aspects of germ cell biology, as well as the opportunity for in vitro manipulation of germ cells for toxicology studies and for genetic intervention.

Keywords

Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
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  2. Isolation and Purification of Primordial (and) Germ Cells

    1. Front Matter

      Pages 1-1
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    2. Isolation and Purification of Viable PGCs from Mouse Embryos

      • Massimo De Felici
      Pages 3-13

    3. Purification and Transfection Methods of Chicken Primordial Germ Cells

      • Luiza Chojnacka-Puchta, Dorota Sawicka
      Pages 15-25

    4. Isolation and In Vitro Propagation of Human Spermatogonial Stem Cells (SSCs)

      • Janmejay Hingu, Guillermo Galdon, Nicholas A. Deebel, Hooman Sadri-Ardekani
      Pages 27-36

    5. Purification by STA-PUT Technique of Male Germ Cells from Single Mouse and RNA-Extraction for Transcriptomic Analysis

      • Chiara Naro, Claudio Sette, Raffaele Geremia
      Pages 37-52

    6. Isolation of Mouse Germ Cells by FACS Using Hoechst 33342 and SYTO16 Double Staining

      • Mark E. Gill, Hubertus Kohler, Antoine H. F. M. Peters
      Pages 53-62

    7. Isolation and In Vitro Culture of Germ Cells and Sertoli Cells from Human Fetal Testis

      • Celine M. Roelse, Arend W. Overeem, Yolanda W. Chang, Meriam Boubakri, Susana M. Chuva de Sousa Lopes
      Pages 63-83

  3. Establishing In Vitro Systems for Studying Primordial (and) Germ Cells

    1. Front Matter

      Pages 85-85
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    2. Human Primordial Germ Cell-Like Cell Induction from Pluripotent Stem Cells by SOX17 and PRDM1 Expression

      • Naoko Irie, Toshihiro Kobayashi, M. Azim Surani
      Pages 87-97

    3. Induction of Primordial Germ Cell-Like Cells from Rat Pluripotent Stem Cells

      • Mami Oikawa, Masumi Hirabayashi, Toshihiro Kobayashi
      Pages 99-111

    4. Induction of Meiotic Initiation in Long-Term Mouse Spermatogonial Stem Cells Under Retinoid Acid and Nutrient Restriction Conditions

      • Xiaoyu Zhang, Ning Wang
      Pages 113-121

    5. Lipofection-Based Delivery of CRISPR/Cas9 Ribonucleoprotein for Gene Editing in Male Germline Stem Cells

      • Mariella Obermeier, Vera Rogiers, Tamara Vanhaecke, Yoni Baert
      Pages 123-134

    6. Mouse In Vitro Spermatogenesis on 3D Bioprinted Scaffolds

      • Guillaume Richer, Tamara Vanhaecke, Vera Rogiers, Ellen Goossens, Yoni Baert
      Pages 135-149

    7. Histological and Cytological Techniques to Study Perinatal Mouse Ovaries and Oocytes

      • Nikoleta Nikou, Maria López Panadés, Ignasi Roig
      Pages 151-170

    8. Method of Isolation and In Vitro Culture of Primordial Follicles in Bovine Animal Model

      • Pritha Dey, Noemi Monferini, Ludovica Donadini, Valentina Lodde, Federica Franciosi, Alberto Maria Luciano
      Pages 171-182

  4. Somatic Reprogramming to Studying Human Aneuploidy Features

    1. Front Matter

      Pages 183-183
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    2. Generation of iPSC Cell Lines from Patients with Sex Chromosome Aneuploidies

      • Veronica Astro, Antonio Adamo
      Pages 185-200

  5. Informatic Tools for Studying Germ Cells Development and Recombination

    1. Front Matter

      Pages 201-201
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    2. Data Analysis Pipeline for scRNA-seq Experiments to Study Early Oogenesis

      • Wei Ge, Teng Zhang, Yang Zhou, Wei Shen
      Pages 203-225
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Editors and Affiliations

  • Department of Biomedicine and Prevention, Section of Human Anatomy, Faculty of Medicine and Surgery, “Tor Vergata“ University of Rome, Rome, Italy

    Marco Barchi

  • Department of Biomedicine and Prevention, Section of Histology and Embryology, Faculty of Medicine and Surgery, “Tor Vergata” University of Rome, Rome, Italy

    Massimo De Felici

Bibliographic Information

  • Book Title: Germ Cell Development

  • Book Subtitle: Methods and Protocols

  • Editors: Marco Barchi, Massimo De Felici

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3698-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3697-8Published: 15 February 2024

  • Softcover ISBN: 978-1-0716-3700-5Due: 17 March 2024

  • eBook ISBN: 978-1-0716-3698-5Published: 14 February 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 288

  • Number of Illustrations: 5 b/w illustrations, 59 illustrations in colour

  • Topics: Developmental Biology, Cell Biology

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