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Germline Stem Cells

Methods and Protocols

  • Book
  • © 2023
  • Latest edition

Overview

Editors:
  1. Michael Buszczak

    1. Department of Molecular Biology, The University of Texas Southwestern Medical Center, Dallas, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2677)

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About this book

This third edition volume expands on the previous editions with new discussion on the latest techniques and model systems used to study germline stem cells. The chapters in this book cover topics such as analysis of the C. elegans germline stem cell pool; live imaging of the Drosophila testis stem cell niche; standardization of single-cell RNA-sequencing analysis workflow to study Drosophila ovary; 3D analysis of the intact mouse ovary; and derivation and primordial germ cell induction of intermediate pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and practical, Germline Stem Cells: Methods and Protocols, Third Edition is a valuable resource for all researchers in the germ stem cell community interested in learning more about this developing field.


Keywords

Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Analysis of the C. elegans Germline Stem Cell Pool

    • Sarah L. Crittenden, Hannah S. Seidel, Judith Kimble
    Pages 1-36

  3. Analysis of C. elegans Germline Small RNA Pathways

    • Mathias S. Renaud, Uri Seroussi, Julie M. Claycomb
    Pages 37-59

  4. Lineage Tracing and Single-Cell RNA-seq in C. elegans to Analyze Transgenerational Epigenetic Phenotypes Inherited from Germ Cells

    • Juan D. Rodriguez, David J. Katz
    Pages 61-79

  5. Methods to Analyze Nutritional and Inter-Organ Control of Drosophila Ovarian Germline Stem Cells

    • Chad Simmons, Tancia W. Bradshaw, Alissa R. Armstrong
    Pages 81-97

  6. Targeting Endogenous Loci That Function in Drosophila Germline Stem Cells

    • Marianne Mercer, Varsha Bhargava, Courtney D. Goldstein, Michael Buszczak
    Pages 99-112

  7. Live Imaging of the Drosophila Testis Stem Cell Niche

    • Leah J. Greenspan, Erika L. Matunis
    Pages 113-125

  8. Enrichment of Undifferentiated Germline and Somatic Cells from Drosophila Testes

    • Sharif M. Ridwan, Matthew Antel, Mayu Inaba
    Pages 127-138

  9. Spermatogonial Dedifferentiation into Germline Stem Cells in Drosophila Testes

    • Salvador C. Herrera, Erika A. Bach
    Pages 139-149

  10. Standardization of Single-Cell RNA-Sequencing Analysis Workflow to Study Drosophila Ovary

    • Deeptiman Chatterjee, Wu-Min Deng
    Pages 151-171

  11. Identification of Germline Stem Cells in Zebrafish

    • Bruce W. Draper
    Pages 173-183

  12. Characterization of the Postnatal Naked Mole-Rat Ovary: From Primordial Germ Cells to Meiotic Prophase I Oocytes

    • Miguel Angel Brieño-Enríquez
    Pages 185-201

  13. A Roadmap for Three-Dimensional Analysis of the Intact Mouse Ovary

    • Bikem Soygur, Mariko H. Foecke, Eliza A. Gaylord, Adam Fries, Jing Li, Ripla Arora et al.
    Pages 203-219

  14. Isolation and Culture Techniques for Fetal Mouse Germ Cells

    • Guillaume Burnet, Josephine Bowles, Cassy M. Spiller
    Pages 221-231

  15. Rattus norvegicus Spermatogenesis Colony-Forming Assays

    • Karen M. Chapman, Ashutosh Pudasaini, Morgan N. Vanderbeck, F. Kent Hamra
    Pages 233-257

  16. Maintenance of Human Primordial Germ Cell-Like Cells in a Long-Term Culture System

    • Joanna J. Gell, Toshi Shioda
    Pages 259-267

  17. Derivation and Primordial Germ Cell Induction of Intermediate Pluripotent Stem Cells

    • Leqian Yu, Emily Ballard, Carlos A. Pinzon Arteaga, Jun Wu
    Pages 269-280

  18. Back Matter

    Pages 281-284
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Editors and Affiliations

  • Department of Molecular Biology, The University of Texas Southwestern Medical Center, Dallas, USA

    Michael Buszczak

Bibliographic Information

  • Book Title: Germline Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Michael Buszczak

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3259-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3258-1Published: 19 July 2023

  • Softcover ISBN: 978-1-0716-3261-1Due: 01 August 2024

  • eBook ISBN: 978-1-0716-3259-8Published: 18 July 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 284

  • Number of Illustrations: 10 b/w illustrations, 52 illustrations in colour

  • Topics: Stem Cells

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