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Emerging Model Organisms

  • Book
  • © 2023

Overview

Editors:
  1. Wei Wang

    1. National Institute of Biological Sciences Beijing, Tsinghua University, Beijing, China

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  2. Nicolas Rohner

    1. Stowers Institute for Medical Research, Kansas City, USA

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    You can also search for this editor in PubMed   Google Scholar

  3. Yongfu Wang

    1. Stowers Institute for Medical Research, Kansas City, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 194)

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About this book

This volume discusses different approaches towards understanding the brain plasticity, aging, and regeneration of the nervous system in model organisms. Chapters guide the reader through an overview of model organisms,  setup of an aquatic system, Tol2-mediated transgenesis, CRISPR/Cas9-mediated gene knock-out and knock-in, Cas13d-mediated gene knockdown, tissue clearing and expansion methods for brain and spinal cord imaging,  Histological techniques, chromatin Immunoprecipitation Sequencing (ChIP-Seq) analysis, single-cell RNA sequencing analysis, BrdU/EdU incorporation assays, brain and spinal cord injury in African killifish, and emerging technologies and future perspectives in African killifish research. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. 

Authoritative and cutting-edge, Emerging Model Organisms aims to be a useful practical guide to researches to help further their study in this field. 

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. African Killifish (Nothobranchius furzeri)

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Establishing a Fishroom for African Turquoise Killifish Nothobranchius furzeri

      • Michelle B. Vilensky, Chi-Kuo Hu
      Pages 3-16

    3. Gene Editing and Transgenic Reporter Assays in African Killifish

      • Wei Wang
      Pages 17-32

    4. Spinal Cord Injury in African Killifish

      • Yufei Lou, Wei Wang
      Pages 33-42

    5. Whole-Brain Clearing and Immunolabelling in the African Killifish Nothobranchius furzeri

      • Augusto Ortega Granillo, Fengyan Deng, Hannah Wilson, Yongfu Wang, Alejandro Sánchez Alvarado
      Pages 43-58

    6. Studying Mating Behaviors in Nothobranchius furzeri

      • Bernadette D. Johnson, Adam G. Jones
      Pages 59-69

    7. The Chromatin Immunoprecipitation (ChIP) Protocol for African Turquoise Killifish

      • Jiaxing Jin, Yixi Yu, Deqing Hu
      Pages 71-79

    8. EdU Labeling of Proliferating Cells in the African Killifish Nothobranchius furzeri

      • Augusto Ortega Granillo, Fengyan Deng, Yongfu Wang, Wei Wang, Alejandro Sánchez Alvarado
      Pages 81-93

    9. Immunofluorescence Staining on Cryosections with High Autofluorescence in African Killifish

      • Shiqi Liu, Wei Wang
      Pages 95-108

    10. Rapid Visualization of Gene Expression Using Chromogenic RNA In Situ Hybridization in African Killifish

      • Mengjun Liu, Shiqi Liu, Wei Wang
      Pages 109-121

    11. Profiling Gene Expression in African Turquoise Killifish Nothobranchius furzeri Embryos with RNA Fluorescence In Situ Hybridization Chain Reaction (HCR)

      • Lu Jia, Chi-Kuo Hu
      Pages 123-141

    12. Cell Dissociation of Tissues for Single-Cell Analysis in African Killifish

      • Liping Zhang, Wei Wang
      Pages 143-151

  3. Mexican Cavefish (Astyanax mexicanus)

    1. Front Matter

      Pages 153-153
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    2. Cryopreservation of the Mexican Tetra Astyanax mexicanus

      • Diana P. Baumann, Andrew Ingalls
      Pages 155-177

    3. Whole-Mount Multicolor Fluorescent Labeling by In Situ Hybridization in Astyanax mexicanus Embryos and Larvae

      • Maryline Blin, Sylvie Rétaux, Jorge Torres-Paz
      Pages 179-192

    4. Application of CRISPR-Cas9 for Functional Analysis in A. mexicanus

      • Itzel Sifuentes-Romero, Estephany Ferrufino, Johanna E. Kowalko
      Pages 193-220

    5. Immunohistochemistry and In Vivo Neural Imaging in A. mexicanus

      • Evan Lloyd, Robert A. Kozol, Erik R. Duboue, Alex C. Keene
      Pages 221-234

    6. Sleep Behavior Analysis in Astyanax mexicanus

      • Pierce Hutton, Evan Lloyd, Madelyn Dotson, Alex C. Keene
      Pages 235-247

    7. A New Method to Analyze Nonvisual-Based Social-Like Interactions in Asocial Cave Fish

      • Motoko Iwashita, Masato Yoshizawa
      Pages 249-262
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Editors and Affiliations

  • National Institute of Biological Sciences Beijing, Tsinghua University, Beijing, China

    Wei Wang

  • Stowers Institute for Medical Research, Kansas City, USA

    Nicolas Rohner, Yongfu Wang

Bibliographic Information

  • Book Title: Emerging Model Organisms

  • Editors: Wei Wang, Nicolas Rohner, Yongfu Wang

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-0716-2875-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2874-4Published: 02 January 2023

  • Softcover ISBN: 978-1-0716-2877-5Published: 30 December 2022

  • eBook ISBN: 978-1-0716-2875-1Published: 01 January 2023

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XV, 359

  • Number of Illustrations: 1 b/w illustrations, 94 illustrations in colour

  • Topics: Neurosciences

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