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Cartilage Tissue Engineering

  • Book
  • © 2023

Overview

Editors:
  1. Martin J. Stoddart

    1. AO Research Institute Davos, Davos Platz, Switzerland

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  2. Elena Della Bella

    1. AO Research Institute Davos, Davos Platz, Switzerland

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    You can also search for this editor in PubMed   Google Scholar

  3. Angela R. Armiento

    1. UCB Pharma, Slough, UK

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2598)

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About this book

This detailed book brings together a collection of methodologies, from the most basic to the more complex, that provides researchers with a platform they can use to embark on a cartilage research career. To aid in the search for novel therapies for cartilage regeneration, this volume addresses 3D cartilage models, challenges associated with RNA and protein extraction, imaging, gene transfer, as well as stable differentiation and variations in cell phenotype from different tissue origins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Cartilage Tissue Engineering serves as an ideal guide for researchers working to advance the vital study of cartilage biology and repair.

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Keywords

Table of contents (28 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Cartilage Tissue Engineering: An Introduction

    • Martin J. Stoddart, Elena Della Bella, Angela R. Armiento
    Pages 1-7

  3. Chondrocyte Isolation and Expansion

    • Paul Harrison, Timothy Hopkins, Charlotte Hulme, Helen McCarthy, Karina Wright
    Pages 9-19

  4. Isolation of Chondrons from Hyaline Cartilage

    • Jasmijn V. Korpershoek, Margot Rikkers, Lucienne A. Vonk
    Pages 21-27

  5. Articular Cartilage Chondroprogenitors: Isolation and Directed Differentiation

    • Ilyas M. Khan, Joshua McKenna, Yadan Zhang
    Pages 29-44

  6. Physioxic Culture of Chondrogenic Cells

    • Girish Pattappa, Brandon D. Markway, Denitsa Docheva, Brian Johnstone
    Pages 45-63

  7. Isolation and In Vitro Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells

    • Angela R. Armiento, Yann D. Ladner, Elena Della Bella, Martin J. Stoddart
    Pages 65-73

  8. Adipose-Derived Stromal Cells: Isolation, Expansion, and Differentiation

    • Justyna M. Buchert, Benedict Lotz, Solvig Diederichs, Wiltrud Richter
    Pages 75-85

  9. Chondrogenic Differentiation of Human-Induced Pluripotent Stem Cells

    • Amanda R. Dicks, Nancy Steward, Farshid Guilak, Chia-Lung Wu
    Pages 87-114

  10. The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulfated Glycosaminoglycans

    • Yann D. Ladner, Mauro Alini, Angela R. Armiento
    Pages 115-121

  11. Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering and Osteoarthritis Therapy

    • Alexander Otahal, Andrea De Luna, Ali Mobasheri, Stefan Nehrer
    Pages 123-140

  12. Metabolomic Profiling to Understand Chondrocyte Metabolism

    • Priyanka P. Brahmachary, Hope D. Welhaven, Ronald K. June
    Pages 141-156

  13. Techniques for Visualization and Quantification of Primary Cilia in Chondrocytes

    • Huan Meng, Clare L. Thompson, Clarissa R. Coveney, Angus K. Wann, Martin M. Knight
    Pages 157-176

  14. Co-culture of Human Articular Chondrocytes Seeded in Polyurethane Scaffolds and Human Mesenchymal Stromal Cells Encapsulated in Alginate Beads

    • Teresa Z. Brose, Yann D. Ladner, E. Johanna Kubosch, Martin J. Stoddart, Angela R. Armiento
    Pages 177-186

  15. Isolation of Murine Articular Chondrocytes for Single-Cell RNA or Bulk RNA Sequencing Analysis

    • Jillian L. McCool, Nicholas R. Hum, Aimy Sebastian, Gabriela G. Loots
    Pages 187-196

  16. Modulation of MicroRNA Expression During In Vitro Chondrogenesis

    • Austin Bell-Hensley, Hongjun Zheng, Audrey McAlinden
    Pages 197-215

  17. Purification and Isolation of Proteins from Hyaline Cartilage

    • Makenna J. Hardy, Xinzhu Pu, Julia Thom Oxford
    Pages 217-225

  18. Histological Preparation and Evaluation of Cartilage Specimens

    • Salim E. Darwiche, Milena Tegelkamp, Katja Nuss, Brigitte von Rechenberg
    Pages 227-263

  19. Articular Cartilage Imaging in the Context of the Superficial Chondrocyte Spatial Organization (SCSO) as a Surrogate Marker for Functional Pathology

    • Bodo Kurz, Thomas Lange, Marita Voelker, Bernd Rolauffs
    Pages 265-270

  20. Unconfined Compression Experimental Protocol for Cartilage Explants and Hydrogel Constructs: From Sample Preparation to Mechanical Characterization

    • Seyed Ali Elahi, Rocío Castro-Viñuelas, Anke Govaerts, Rik Lories, Nele Famaey, Ilse Jonkers
    Pages 271-287
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Editors and Affiliations

  • AO Research Institute Davos, Davos Platz, Switzerland

    Martin J. Stoddart, Elena Della Bella

  • UCB Pharma, Slough, UK

    Angela R. Armiento

Bibliographic Information

  • Book Title: Cartilage Tissue Engineering

  • Editors: Martin J. Stoddart, Elena Della Bella, Angela R. Armiento

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2839-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2838-6Published: 11 November 2022

  • Softcover ISBN: 978-1-0716-2841-6Published: 12 November 2023

  • eBook ISBN: 978-1-0716-2839-3Published: 10 November 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 393

  • Number of Illustrations: 11 b/w illustrations, 105 illustrations in colour

  • Topics: Regenerative Medicine/Tissue Engineering

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