Extended Data Fig. 2: Effect of light schedule on AgRP-neuron rhythms.
a. Average locomotor activity (left) and food intake (right) rhythms in the first days of constant dark (DD, left, 6 mice). b. Average locomotor activity, food intake, AgRP GCaMP rhythms (5/6 mice were still rhythmic) under AL conditions, Rayleigh-plots (ad lib and fasted phases differ significantly, p = 0.0329, circular one-way ANOVA), and AgRP activity under fasted conditions, respectively, in later days of DD light setting. c. Average locomotor activity (left) and food intake (middle) rhythms in the first days of constant light (LL, 8 mice) and AgRP GCaMP activity on 10th day of LL setting (right). 0/7 animals were rhythmic. d-f. Two-tailed Pearson correlation between average food intake and locomotor activity across same circadian time points under 12 h Light :12 h Dark (d), DD day 9 (e), and LL day 10 (f). g,h. Average AgRP-neuron activity on baseline day (BL, 7 mice) overlaid with the first jetlag day (JD-1, 6 mice) (g) and quantification of post light phase shift mid-dark phase and mid-light phase average AgRP activities (h) in AL fed mice. i,j. Same as (g,h) except no food was provided (8 BL, 6 JD-1 mice). Data are presented as mean ± SEM. For exact P-values and statistics, see Extended Data Fig. 2 Source Data.