Extended Data Fig. 9: DMHPDYN neurons provide timed disinhibition to AgRP-neurons in TRF.
a. Left: Schematic of in vivo fiber photometry recording of PDYN neuronal Ca2+ activity in DMH. Right: Diagram describing TRF setting. b,c. Heatmap showing individual (b) and line graph showing averaged (c) DMHPDYN activity in mice under TRF between ZT3-7. n = 6 mice. d. Average DMHPDYN activity in B1 (ZT21-0), B2(ZT0-3) and Post (ZT4-7) periods in TRF and no food access days. Two-way ANOVA with Tukey’s multiple comparison analysis (No food, B1 vs Post, p = 0.012, B2 vs Post p = 0.016, n = 6 mice). e. Change in DMHPDYN activity (Δ = z-score on TRF day – z-score on no food access day from c) overlaid with ARCNPY/AgRP (from Extended Data Figure 8i). Data are presented as mean ± SEM. For exact P values and data, see Extended Data 9 Source Data.