Extended Data Figure 7: Wnt defects in ctnnd2b zebrafish morphant embryos.

a, Relative axin2 mRNA level in the ten-somite stage in control versus morphant embryos. b, Wholemount RNA in situ hybridization of chordin. Dorsal view in upper panels with the anterior aspect at the apex. The dorsal axis is marked with a red dashed line, and regions with high expression are marked (arrows) in control embryos. Lateral view in lower panels, length (L) and width (W) of chordin expression domains were measured. c, Quantification of chordin expression domains (length:width ratio) in injected embryos. d, Immunoblot showing a macromolecular interaction between Flag-tagged CTNNB1 and GFP-tagged CTNND2 with the corresponding variants. Two-sided t-tests were conducted: P < 0.05, P < 0.01 and P < 0.001, respectively. Sample size (n) is marked for each condition.