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Effect of Conditioned Medium from Human Adipose-Derived Mesenchymal Stem Cells on Human Sperm Quality During Cryopreservation

  • Reproductive Biology: Original Article
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Abstract

The cryopreservation procedure decreases sperm quality, causing certain changes at structural and molecular levels affecting fertilizing ability. We aimed to investigate the impacts of human adipose-derived mesenchymal stem cells (HAd-MSCs) conditioned medium (CM) on the protection of human sperm from cryoinjury. Thirty normal semen specimens were evaluated in this study. Each specimen was separated into six groups and enhanced with varying concentrations of human Ad-MSCs-CM (0, 10, 30, 50, 70, and 100%). Sperm motility, viability, morphology, apoptosis, mitochondrial potential, and lipid peroxidation, and DNA fragmentation were evaluated before freezing and after thawing. The results showed that the total motility was preserved in 10% human Ad-MSCs-CM group. Also, DNA fragmentation was significantly lower in 10% compared to 0% human Ad-MSCs-CM (63.62 ± 17.72% vs.76.46 ± 4.87%, respectively, P < 0.004). Human Ad-MSCs-CM in groups of 10, 30, 50, and 70% reduced lipid peroxidation. The normal sperm morphology rate, mitochondrial membrane potential, and apoptosis showed no significant differences across various groups. It seems that human Ad-MSCs-CM can protect the sperm parameters during the cryopreservation by decreasing cryoinjury.

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Acknowledgements

The authors would like to thank Iran University of Medical Sciences for financial support (99-3-99-19549).

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Authors and Affiliations

  1. Department of Physiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

    Hanieh Ghasemian Nafchi & Yaser Azizi

  2. Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Iman Halvaei

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  1. Hanieh Ghasemian Nafchi
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  2. Yaser Azizi
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  3. Iman Halvaei
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Contributions

HGN performed the study and drafted the manuscript, YA participated in the study design coordination and revised the manuscript, IH designed the study, performed the statistical analysis, and revised the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Yaser Azizi or Iman Halvaei.

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The ethics committee of the authors’ institute approved this study (IR.IUMS.REC.1399.1408).

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Nafchi, H.G., Azizi, Y. & Halvaei, I. Effect of Conditioned Medium from Human Adipose-Derived Mesenchymal Stem Cells on Human Sperm Quality During Cryopreservation. Reprod. Sci. 31, 1586–1592 (2024). https://doi.org/10.1007/s43032-024-01505-y

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