Abstract
Human milk is a remarkable biofluid that provides essential nutrients and immune protection to newborns. Breastfeeding women consuming medications could pass the drug through their milk to neonates. Drugs can be transferred to human milk by passive diffusion or active transport. The physicochemical properties of the drug largely impact the extent of drug transfer into human milk. A comprehensive understanding of the physiology of human milk formation, composition of milk, mechanisms of drug transfer, and factors influencing drug transfer into human milk is critical for appropriate selection and use of medications in lactating women. Quantification of drugs in the milk is essential for assessing the safety of pharmacotherapy during lactation. This can be achieved by developing specific, sensitive, and reproducible analytical methods using techniques such as liquid chromatography coupled with mass spectrometry. The present review briefly discusses the physiology of human milk formation, composition of human milk, mechanisms of drug transfer into human milk, and factors influencing transfer of drugs from blood to milk. We further expand upon and critically evaluate the existing analytical approaches/assays used for the quantification of drugs in human milk.
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References
Ayad M, Costantine MM. Epidemiology of medications use in pregnancy. Semin Perinatol. 2015;39(7):508–11.
Saha MR, Ryan K, Amir LH. Postpartum women’s use of medicines and breastfeeding practices: a systematic review. Int Breastfeed J. 2015;10:28.
Ito S, Lee A. Drug excretion into breast milk–overview. Adv Drug Deliv Rev. 2003;55(5):617–27.
Friguls B, Joya X, Garcia-Algar O, et al. A comprehensive review of assay methods to determine drugs in breast milk and the safety of breastfeeding when taking drugs. Anal Bioanal Chem. 2010;397(3):1157–79.
Wang J, Johnson T, Sahin L, et al. Evaluation of the safety of drugs and biological products used during lactation: workshop summary. Clin Pharmacol Ther. 2017;101(6):736–44.
Shapiro RL, Holland DT, Capparelli E, et al. Antiretroviral concentrations in breast-feeding infants of women in Botswana receiving antiretroviral treatment. J Infect Dis. 2005;192(5):720–7.
Ballard O, Morrow AL. Human milk composition: nutrients and bioactive factors. Pediatr Clin N Am. 2013;60(1):49–74.
van Sadelhoff JHJ, Mastorakou D, Weenen H, et al. Short communication: differences in levels of free amino acids and total protein in human foremilk and hindmilk. Nutrients. 2018;10(12):1828.
Lopes BR, Barreiro JC, Cass QB. Bioanalytical challenge: a review of environmental and pharmaceuticals contaminants in human milk. J Pharm Biomed. 2016;25(130):318–25.
Hassiotou F, Geddes D. Anatomy of the human mammary gland: current status of knowledge. Clin Anat. 2013;26(1):29–48.
McManaman JL, Neville MC. Mammary physiology and milk secretion. Adv Drug Deliv Rev. 2003;55(5):629–41.
Pang WW, Hartmann PE. Initiation of human lactation: secretory differentiation and secretory activation. J Mammary Gland Biol Neoplasia. 2007;12(4):211–21.
Quezada A, Vafai K. Modeling and analysis of transport in the mammary glands. Phys Biol. 2014;11(4): 045004.
Macias H, Hinck L. Mammary gland development. Wiley Interdiscip Rev Dev Biol. 2012;1(4):533–57.
Fleishaker JC. Models and methods for predicting drug transfer into human milk. Adv Drug Deliv Rev. 2003;55(5):643–52.
Godhia ML, Patel N. Colostrum-its composition, benefits as a nutraceutical - a review. Curr Res Nutr Food Sci. 2013;1(1):37–47.
Kim SY, Yi DY. Components of human breast milk: from macronutrient to microbiome and microRNA. Clin Exp Pediatr. 2020;63(8):301–9.
Andreas NJ, Kampmann B, Mehring L-D. Human breast milk: a review on its composition and bioactivity. Early Hum Dev. 2015;91(11):629–35.
Stowe ZN, Hostetter AL, Owens MJ, et al. The pharmacokinetics of sertraline excretion into human breast milk: determinants of infant serum concentrations. J Clin Psychiatry. 2003;64(1):73–80.
García-Lino AM, Álvarez-Fernández I, Blanco-Paniagua E, et al. Transporters in the mammary gland-contribution to presence of nutrients and drugs into milk. Nutrients. 2019;11(10):2372.
Atkinson HC, Begg EJ. Prediction of drug distribution into human milk from physicochemical characteristics. Clin Pharmacokinet. 1990;18(2):151–67.
JTW. Drugs in Breast Milk, 2nd ed. In: ADIS; 1981.
Ito S, Alcorn J. Xenobiotic transporter expression and function in the human mammary gland. Adv Drug Deliv Rev. 2003;55(5):653–65.
Hodel EM, Marzolini C, Waitt C, et al. Pharmacokinetics, placental and breast milk transfer of antiretroviral drugs in pregnant and lactating women living with HIV. Curr Pharm Des. 2019;25(5):556–76.
Gao B, Vavricka SR, Meier PJ, et al. Differential cellular expression of organic anion transporting peptides OATP1A2 and OATP2B1 in the human retina and brain: implications for carrier-mediated transport of neuropeptides and neurosteriods in the CNS. Pflugers Arch. 2015;467(7):1481–93.
Seaton S, Reeves M, McLean S. Oxycodone as a component of multimodal analgesia for lactating mothers after Caesarean section: relationships between maternal plasma, breast milk and neonatal plasma levels. Aus NZJ Obstet Gynaecol. 2007;47(3):181–5.
Malfara BN, Benzi JRL, de Oliveira Filgueira GC, et al. ABCG2 c.421C>A polymorphism alters nifedipine transport to breast milk in hypertensive breastfeeding women. Reprod Toxicol. 2019;85:1–5.
Jonker JW, Merino G, Musters S, et al. The breast cancer resistance protein BCRP (ABCG2) concentrates drugs and carcinogenic xenotoxins into milk. Nat Med. 2005;11(2):127–9.
Banta-Wright SA. Minimizing infant exposure to and risks from medications while breastfeeding. J Perinat Neonatal Nurs. 1997;11(2):71–84 (quiz 85-6).
Breitzka RL, Sandritter TL, Hatzopoulos FK. Principles of drug transfer into breast milk and drug disposition in the nursing infant. J Hum Lact. 1997;13(2):155–8.
Lipinski CA, Lombardo F, Dominy BW, et al. Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings. Adv Drug Deliv Rev. 2001;46(1–3):3–26.
Development. NIoCHaH. Drugs and Lactation Database (LactMed(R)). 2021. https://www.ncbi.nlm.nih.gov/books/NBK501922/. Accessed 1 Dec 2023.
Rivera-calimlim L. Drugs in breast milk. Drug Ther (NY). 1977;7(12):59–63.
Özdemir Z, Traş B. Behaviours of drugs in the milk - a review. Vet Sci Prac. 2018;13(3):364–72.
Sisodia CS, Stowe CM. The mechanism of drug secretion into bovine milk. Ann NY Acad Sci. 1964;24(111):650–61.
Wilson JT, Brown RD, Cherek DR, et al. Drug excretion in human breast milk: principles, pharmacokinetics and projected consequences. Clin Pharmacokinet. 1980;5(1):1–66.
Syversen GB, Ratkje SK. Drug distribution within human milk phases. J Pharm Sci. 1985;74(10):1071–4.
Smith JA, Morgan JR, Rachlis AR, et al. Clindamycin in human breast milk. Can Med Assoc J. 1975;112(7):806.
Cahill JB Jr, Bailey EM, Chien S, et al. Levofloxacin secretion in breast milk: a case report. Pharmacotherapy. 2005;25(1):116–8.
Khurana R, Bin Jardan YA, Wilkie J, et al. Breast milk concentrations of amiodarone, desethylamiodarone, and bisoprolol following short-term drug exposure: two case reports. J Clin Pharmacol. 2014;54(7):828–31.
Lutz UC, Wiatr G, Orlikowsky T, et al. Olanzapine treatment during breast feeding: a case report. Ther Drug Monit. 2008;30(3):399–401.
Rowe HE, Felkins K, Cooper SD, et al. Transfer of linezolid into breast milk. J Hum Lact. 2014;30(4):410–2.
Teoh S, Ilett KF, Hackett LP, et al. Estimation of rac-amisulpride transfer into milk and of infant dose via milk during its use in a lactating woman with bipolar disorder and schizophrenia. Breastfeed Med. 2011;6(2):85–8.
Hill RC, McIvor RJ, Wojnar-Horton RE, et al. Risperidone distribution and excretion into human milk: case report and estimated infant exposure during breast-feeding. J Clin Psychopharmacol. 2000;20(2):285–6.
Molad M, Ashkenazi L, Gover A, et al. Melatonin stability in human milk. Breastfeed Med. 2019;14(9):680–2.
Peakman T, Elliott P. Current standards for the storage of human samples in biobanks. Genome Med. 2010;2(10):72.
Collecting, storing, accessing, and protecting biospecimens and biodata. In: Robert M. Hauser MW, Robert Pool, and Barney Cohen, editor. Conducting Biosocial Surveys. Washington DC: National Academic Press; 2010. p. 19-40.
Rodríguez-Gómez R, Dorival-García N, Zafra-Gómez A, et al. New method for the determination of parabens and bisphenol A in human milk samples using ultrasound-assisted extraction and clean-up with dispersive sorbents prior to UHPLC-MS/MS analysis. J Chromatogr B Analyt Technol Biomed Life Sci. 2015;15(992):47–55.
McCarthy JJ, Posey BL. Methadone levels in human milk. J Hum Lact. 2000;16(2):115–20.
Alvim J Jr, Lopes BR, Cass QB. Simultaneous enantioselective quantification of fluoxetine and norfluoxetine in human milk by direct sample injection using 2-dimensional liquid chromatography-tandem mass spectrometry. J Chromatogr A. 2016;17(1451):120–6.
Lopes BR, Barreiro JC, Baraldi PT, et al. Quantification of carbamazepine and its active metabolite by direct injection of human milk serum using liquid chromatography tandem ion trap mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2012;15(889–890):17–23.
El-Gindy A, Nassar M, Attia K, et al. High-performance liquid chromatographic determination of ethamsylate in human breast milk. J Liq Chromatogr Relat. 2013;36:1915–30.
El-Gindy A, Sallam S, Abdel-Salam RA. HPLC method for the simultaneous determination of atenolol and chlorthalidone in human breast milk. J Sep Sci. 2008;31(4):677–82.
Hadad GM, Abdel Salam RA, Emara S. Validated and optimized high-performance liquid chromatographic determination of tizoxanide, the main active metabolite of nitazoxanide in human urine, plasma and breast milk. J Chromatogr Sci. 2012;50(6):509–15.
Rezk NL, Abdel-Megeed MF, Kashuba AD. Development of a highly efficient extraction technique and specific multiplex assay for measuring antiretroviral drug concentrations in breast milk. Ther Drug Monit. 2007;29(4):429–36.
Manohar M, Marzinke MA. Validation and implementation of an ultrasensitive liquid chromatographic-tandem mass spectrometric (LC-MS/MS) assay for dapivirine quantitation in breast milk. Clin Biochem. 2020;82:66–72.
Palombi L, Pirillo MF, Andreotti M, et al. Antiretroviral prophylaxis for breastfeeding transmission in Malawi: drug concentrations, virological efficacy and safety. Antivir Ther. 2012;17(8):1511–9.
Ramirez-Ramirez A, Sanchez-Serrano E, Loaiza-Flores G, et al. Simultaneous quantification of four antiretroviral drugs in breast milk samples from HIV-positive women by an ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method. PLoS ONE. 2018;13(1): e0191236.
Rezk NL, White N, Bridges AS, et al. Studies on antiretroviral drug concentrations in breast milk: validation of a liquid chromatography-tandem mass spectrometric method for the determination of 7 anti-human immunodeficiency virus medications. Ther Drug Monit. 2008;30(5):611–9.
Bartu A, Dusci LJ, Ilett KF. Transfer of methylamphetamine and amphetamine into breast milk following recreational use of methylamphetamine. Br J Clin Pharmacol. 2009;67(4):455–9.
Rigourd V, de Villepin B, Amirouche A, et al. Ibuprofen concentrations in human mature milk–first data about pharmacokinetics study in breast milk with AOR-10127 “Antalait” study. Ther Drug Monit. 2014;36(5):590–6.
Wei B, McGuffey JE, Blount BC, et al. Sensitive Quantification of cannabinoids in milk by alkaline saponification-solid phase extraction combined with isotope dilution UPLC-MS/MS. ACS Omega. 2016;1(6):1307–13.
Ramnarine RS, Poklis JL, Wolf CE. Determination of cannabinoids in breast milk using QuEChERS and ultra-performance liquid chromatography and tandem mass spectrometry. J Anal Toxicol. 2019;43(9):746–52.
van Leeuwen SS. Challenges and pitfalls in human milk oligosaccharide analysis. Nutrients. 2019;11(11):2684.
Robinson RC, Colet E, Tian T, et al. An improved method for the purification of milk oligosaccharides by graphitised carbon-solid phase extraction. Int Dairy J. 2018;80:62–8.
Dodd S, Stocky A, Buist A, et al. Sertraline in paired blood plasma and breast-milk samples from nursing mothers. Hum Psychopharmacol. 2000;15(4):161–264.
Newport DJ, Pennell PB, Calamaras MR, et al. Lamotrigine in breast milk and nursing infants: determination of exposure. Pediatrics. 2008;122(1):e223–31.
Purkiewicz A, Pietrzak-Fiecko R, Sorgel F, et al. Caffeine, paraxanthine, theophylline, and theobromine content in human milk. Nutrients. 2022;14(11):2196.
Ilett KF, Kristensen JH, Hackett LP, et al. Distribution of venlafaxine and its O-desmethyl metabolite in human milk and their effects in breastfed infants. Br J Clin Pharmacol. 2002;53(1):17–22.
Kristensen JH, Ilett KF, Rampono J, et al. Transfer of the antidepressant mirtazapine into breast milk. Br J Clin Pharmacol. 2007;63(3):322–7.
Kociszewska-Najman B, Mazanowska N, Borek-Dzieciol B, et al. Low content of cyclosporine A and its metabolites in the colostrum of post-transplant mothers. Nutrients. 2020;12(9):2713.
Kociszewska-Najman B, Mazanowska N, Pietrzak B, et al. Low transfer of tacrolimus and its metabolites into colostrum of graft recipient mothers. Nutrients. 2018;10(3):267.
Wan EW, Davey K, Page-Sharp M, et al. Dose-effect study of domperidone as a galactagogue in preterm mothers with insufficient milk supply, and its transfer into milk. Br J Clin Pharmacol. 2008;66(2):283–9.
Begg EJ, Malpas TJ, Hackett LP, et al. Distribution of R- and S-methadone into human milk during multiple, medium to high oral dosing. Br J Clin Pharmacol. 2001;52(6):681–5.
Page-Sharp M, Hale TW, Hackett LP, et al. Measurement of nicotine and cotinine in human milk by high-performance liquid chromatography with ultraviolet absorbance detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2003;796(1):173–80.
Amundsen S, Nordeng H, Fuskevag OM, et al. Transfer of triptans into human breast milk and estimation of infant drug exposure through breastfeeding. Basic Clin Pharmacol Toxicol. 2021;128(6):795–804.
Benaboud S, Pruvost A, Coffie PA, et al. Concentrations of tenofovir and emtricitabine in breast milk of HIV-1-infected women in Abidjan, Cote d’Ivoire, in the ANRS 12109 TEmAA Study, Step 2. Antimicrob Agents Chemother. 2011;55(3):1315–7.
Schneider S, Peltier A, Gras A, et al. Efavirenz in human breast milk, mothers’, and newborns’ plasma. J Acquir Immune Defic Syndr. 2008;48(4):450–4.
Croke S, Buist A, Hackett LP, et al. Olanzapine excretion in human breast milk: estimation of infant exposure. Int J Neuropsychopharmacol. 2002;5(3):243–7.
Pellegrini M, Marchei E, Rossi S, et al. Liquid chromatography/electrospray ionization tandem mass spectrometry assay for determination of nicotine and metabolites, caffeine and arecoline in breast milk. Rapid Commun Mass Spectrom. 2007;21(16):2693–703.
Grimm D, Pauly E, Poschl J, et al. Buprenorphine and norbuprenorphine concentrations in human breast milk samples determined by liquid chromatography-tandem mass spectrometry. Ther Drug Monit. 2005;27(4):526–30.
Hostetter AL, Stowe ZN, Cox M, et al. A novel system for the determination of antidepressant concentrations in human breast milk. Ther Drug Monit. 2004;26(1):47–52.
Ilett KF, Paech MJ, Page-Sharp M, et al. Use of a sparse sampling study design to assess transfer of tramadol and its O-desmethyl metabolite into transitional breast milk. Br J Clin Pharmacol. 2008;65(5):661–6.
Law I, Ilett KF, Hackett LP, et al. Transfer of chloroquine and desethylchloroquine across the placenta and into milk in Melanesian mothers. Br J Clin Pharmacol. 2008;65(5):674–9.
Misri S, Kim J, Riggs KW, et al. Paroxetine levels in postpartum depressed women, breast milk, and infant serum. J Clin Psychiatry. 2000;61(11):828–32.
Franssen EJ, Meijs V, Ettaher F, et al. Citalopram serum and milk levels in mother and infant during lactation. Ther Drug Monit. 2006;28(1):2–4.
Jacqz-Aigrain E, Serreau R, Boissinot C, et al. Excretion of ketoprofen and nalbuphine in human milk during treatment of maternal pain after delivery. Ther Drug Monit. 2007;29(6):815–8.
Kristensen JH, Hackett LP, Kohan R, et al. The amount of fluvoxamine in milk is unlikely to be a cause of adverse effects in breastfed infants. J Hum Lact. 2002;18(2):139–43.
Rampono J, Hackett LP, Kristensen JH, et al. Transfer of escitalopram and its metabolite demethylescitalopram into breastmilk. Br J Clin Pharmacol. 2006;62(3):316–22.
Rampono J, Kristensen JH, Hackett LP, et al. Citalopram and demethylcitalopram in human milk; distribution, excretion and effects in breast fed infants. Br J Clin Pharmacol. 2000;50(3):263–8.
Taghizadeh M, Ebrahimi M, Fooladi E, et al. Preconcentration and determination of five antidepressants from human milk and urine samples by stir bar filled magnetic ionic liquids using liquid-liquid-liquid microextraction-high-performance liquid chromatography. J Sep Sci. 2022;45(8):1434–44.
Ahmad Panahi H, Ejlali M, Chabouk M. Two-phase and three-phase liquid-phase microextraction of hydrochlorothiazide and triamterene in urine samples. Biomed Chromatogr. 2016;30(7):1022–8.
Li GZ, Row KH. Recent applications of molecularly imprinted polymers (MIPs) on micro-extraction techniques. Sep Purif Rev. 2018;47(1):1–18.
Kul A, Sagirli O. Determination of cefuroxime in breast milk by LC-MS/MS using SALLME technique. Biomed Chromatogr. 2023;12: e5744.
Kiriazopoulos E, Zaharaki S, Vonaparti A, et al. Quantification of three beta-lactam antibiotics in breast milk and human plasma by hydrophilic interaction liquid chromatography/positive-ion electrospray ionization mass spectrometry. Drug Test Anal. 2017;9(7):1062–72.
Kole PL, Venkatesh G, Kotecha J, et al. Recent advances in sample preparation techniques for effective bioanalytical methods. Biomed Chromatogr. 2011;25(1–2):199–217.
Lindemalm S, Nydert P, Svensson JO, et al. Transfer of buprenorphine into breast milk and calculation of infant drug dose. J Hum Lact. 2009;25(2):199–205.
Lemmer P, Schneider S, Muhe A, et al. Quantification of lorazepam and lormetazepam in human breast milk using GC-MS in the negative chemical ionization mode. J Anal Toxicol. 2007;31(4):224–6.
Gjerde J, Kjellevold M, Dahl L, et al. Validation and determination of 25(OH) vitamin D and 3-Epi25(OH)D3 in breastmilk and maternal- and infant plasma during breastfeeding. Nutrients. 2020;12(8):2271.
Karunanithi D, Radhakrishna A, Sivaraman KP, et al. Quantitative determination of melatonin in milk by LC-MS/MS. J Food Sci Technol. 2014;51(4):805–12.
Marchei E, Escuder D, Pallas CR, et al. Simultaneous analysis of frequently used licit and illicit psychoactive drugs in breast milk by liquid chromatography tandem mass spectrometry. J Pharm Biomed. 2011;55(2):309–16.
Nitsun M, Szokol JW, Saleh HJ, et al. Pharmacokinetics of midazolam, propofol, and fentanyl transfer to human breast milk. Clin Pharmacol Ther. 2006;79(6):549–57.
Haas JS, Kaplan CP, Barenboim D, et al. Bupropion in breast milk: an exposure assessment for potential treatment to prevent post-partum tobacco use. Tob Control. 2004;13(1):52–6.
Winecker RE, Goldberger BA, Tebbett IR, et al. Detection of cocaine and its metabolites in breast milk. J Forensic Sci. 2001;46(5):1221–3.
Aresta A, Palmisano F, Zambonin CG. Simultaneous determination of caffeine, theobromine, theophylline, paraxanthine and nicotine in human milk by liquid chromatography with diode array UV detection. Food Chem. 2005;93(1):177–81.
Swortwood MJ, Scheidweiler KB, Barnes AJ, et al. Simultaneous quantification of buprenorphine, naloxone and phase I and II metabolites in plasma and breastmilk by liquid chromatography-tandem mass spectrometry. J Chromatogr A. 2016;13(1446):70–7.
Polson C, Sarkar P, Incledon B, et al. Optimization of protein precipitation based upon effectiveness of protein removal and ionization effect in liquid chromatography-tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2003;785(2):263–75.
Weisskopf E, Panchaud A, Nguyen KA, et al. Simultaneous determination of selective serotonin reuptake inhibitors and their main metabolites in human breast milk by liquid chromatography-electrospray mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1(1057):101–9.
Shahane A, Zhao W, Pakalapati N, et al. Simultaneous quantitation of ketamine, norketamine and dehydronorketamine in human milk using a novel ultra high-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) assay. J Pharm Biomed. 2023;1(234): 115502.
Dei Cas M, Casagni E, Gambaro V, et al. Determination of daptomycin in human plasma and breast milk by UPLC/MS-MS. J Chromatogr B Analyt Technol Biomed Life Sci. 2019;15(1116):38–43.
Jin W, Gui J, Li G, et al. High-throughput quantitation of trace level melatonin in human milk by on-line enrichment liquid chromatography-tandem mass spectrometry. Anal Chim Acta. 2021;1(1176): 338764.
Lwin EMP, Gerber C, Song Y, et al. A new LC-MS/MS bioanalytical method for perindopril and perindoprilat in human plasma and milk. Anal Bioanal Chem. 2017;409(26):6141–8.
Lwin EMP, Leggett C, Ritchie U, et al. Transfer of rosuvastatin into breast milk: liquid chromatography-mass spectrometry methodology and clinical recommendations. Drug Des Dev Ther. 2018;12:3645–51.
Nikolaou P, Papoutsis I, Athanaselis S, et al. Development and validation of a method for the determination of buprenorphine and norbuprenorphine in breast milk by gas chromatography-mass spectrometry. Biomed Chromatogr. 2012;26(3):358–62.
Nikolaou PD, Papoutsis II, Maravelias CP, et al. Development and validation of an EI-GC-MS method for the determination of methadone and its major metabolites (EDDP and EMDP) in human breast milk. J Anal Toxicol. 2008;32(7):478–84.
Salazar FR, D’Avila FB, de Oliveira MH, et al. Development and validation of a bioanalytical method for five antidepressants in human milk by LC-MS. J Pharm Biomed. 2016;10(129):502–8.
Waitt C, Diliiy Penchala S, Olagunju A, et al. Development, validation and clinical application of a method for the simultaneous quantification of lamivudine, emtricitabine and tenofovir in dried blood and dried breast milk spots using LC-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;15(1060):300–7.
Wegler C, Saleh A, Lindqvist A, et al. Simple and rapid quantification of cetirizine, venlafaxine, and O-desmethylvenlafaxine in human breast milk, and metformin in human milk and plasma with UHPLC-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 2022;1(1205): 123340.
Zhang M, Moore GA, Lever M, et al. Rapid and simple high-performance liquid chromatographic assay for the determination of metformin in human plasma and breast milk. J Chromatogr B Analyt Technol Biomed Life Sci. 2002;766(1):175–9.
Wollein U, Schech B, Hardt J, et al. Determination and quantitation of sildenafil and its major metabolite in the breast milk of a lactating woman. J Pharm Biomed. 2016;20(120):100–5.
Furugen A, Nishimura A, Umazume T, et al. Simple and validated method to quantify lacosamide in human breast milk and plasma using UPLC/MS/MS and its application to estimate drug transfer into breast milk. J Pharm Health Care Sci. 2023;9(1):26.
Choo RE, Jansson LM, Scheidweiler K, et al. A validated liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometric method for the quantification of methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), and 2-Ethyl-5-methyl-3,3-diphenylpyroline (EMDP) in human breast milk. J Anal Toxicol. 2007;31(5):265–9.
Mkhize B, Kellermann T, Norman J, et al. Validation and application of a quantitative liquid chromatography tandem mass spectrometry assay for the analysis of rifapentine and 25-O-desacetyl rifapentine in human milk. J Pharm Biomed. 2022;5(215): 114774.
Sempio C, Wymore E, Palmer C, et al. Detection of cannabinoids by LC-MS-MS and ELISA in breast milk. J Anal Toxicol. 2021;45(7):686–92.
Yeniceli D, Dogrukol-Ak D, Tuncel M. A Validated HPLC Method with fluorescence detection for the determination of droperidol in pharmaceutical tablets, human serum, and human milk. Chromatographia. 2007;66:S37–43.
Fraissinet F, Oumar AA, Seraissol P, et al. Method validation and clinical application for the quantification of lopinavir, efavirenz, and ritonavir in breast milk using liquid chromatography tandem mass spectrometry. J Mass Spectrom. 2022;57(12): e4897.
Zuma P, Joubert A, van der Merwe M, et al. Validation and application of a quantitative LC-MS/MS assay for the analysis of first-line anti-tuberculosis drugs, rifabutin and their metabolites in human breast milk. J Chromatogr B Analyt Technol Biomed Life Sci. 2022;15(1211): 123489.
Monfort A, Jutras M, Martin B, et al. Simultaneous quantification of 19 analytes in breast milk by liquid chromatography-tandem mass spectrometry (LC-MS/MS). J Pharm Biomed. 2021;10(204): 114236.
Marson ME, Padro JM, Reta MR, et al. A simple and efficient HPLC method for benznidazole dosage in human breast milk. Ther Drug Monit. 2013;35(4):522–6.
Markopoulou CK, Koundourellis JE. Development of a validated liquid chromatography method for the simultaneous determination of ethinyl estradiol, cyproterone acetate, and norgestrel in breast milk following solid-phase extraction. J Liq Chromatog. 2006;29:685–700.
Lopes BR, Cassiano NM, Carvalho DM, et al. Simultaneous quantification of fluoxetine and norfluoxetine in colostrum and mature human milk using a 2-dimensional liquid chromatography-tandem mass spectrometry system. J Pharm Biomed. 2018;20(150):362–7.
Wolfson P, Cole R, Lynch K, et al. The pharmacokinetics of ketamine in the breast milk of lactating women: quantification of ketamine and metabolites. J Psychoactive Drugs. 2022;26:1–5.
Ansermot N, Brawand-Amey M, Eap CB. Simultaneous quantification of selective serotonin reuptake inhibitors and metabolites in human plasma by liquid chromatography-electrospray mass spectrometry for therapeutic drug monitoring. J Chromatogr B Analyt Technol Biomed Life Sci. 2012;15(885–886):117–30.
Tiris G, Gazioglu I, Furton KG, et al. Fabric phase sorptive extraction combined with high performance liquid chromatography for the determination of favipiravir in human plasma and breast milk. J Pharm Biomed. 2023;20(223): 115131.
Kabir A, Samanidou V. Fabric phase sorptive extraction: a paradigm shift approach in analytical and bioanalytical sample preparation. Molecules. 2021;26(4):865.
Leon-Gonzalez ME, Rosales-Conrado N. Determination of ibuprofen enantiomers in breast milk using vortex-assisted matrix solid-phase dispersion and direct chiral liquid chromatography. J Chromatogr A. 2017;8(1514):88–94.
Olagunju A, Amara A, Waitt C, et al. Validation and clinical application of a method to quantify nevirapine in dried blood spots and dried breast-milk spots. J Antimicrob Chemother. 2015;70(10):2816–22.
Olagunju A, Bolaji OO, Amara A, et al. Development, validation and clinical application of a novel method for the quantification of efavirenz in dried breast milk spots using LC-MS/MS. J Antimicrob Chemother. 2015;70(2):555–61.
Eyal S, Kim JD, Anderson GD, et al. Atenolol pharmacokinetics and excretion in breast milk during the first 6 to 8 months postpartum. J Clin Pharmacol. 2010;50(11):1301–9.
Hebert MF, Carr DB, Anderson GD, et al. Pharmacokinetics and pharmacodynamics of atenolol during pregnancy and postpartum. J Clin Pharmacol. 2005;45(1):25–33.
Sagirli O, Demirci S, Onal A. A very simple high-performance liquid chromatographic method with fluorescence detection for the determination of gemifloxacin in human breast milk. Luminescence. 2015;30(8):1326–9.
Kim J, Riggs KW, Misri S, et al. Stereoselective disposition of fluoxetine and norfluoxetine during pregnancy and breast-feeding. Br J Clin Pharmacol. 2006;61(2):155–63.
Phyo Lwin EM, Gerber C, Song Y, et al. A new LC-MS/MS bioanalytical method for atenolol in human plasma and milk. Bioanalysis. 2017;9(7):517–30.
Mirochnick M, Thomas T, Capparelli E, et al. Antiretroviral concentrations in breast-feeding infants of mothers receiving highly active antiretroviral therapy. Antimicrob Agents Chemother. 2009;53(3):1170–6.
US Department of Health and Human Services. Food and Drug Administration. Bioanalytical method validation guidance for industry. 2018. Available online: https://www.fda.gov/files/drugs/published/Bioanalytical-Method-Validation-Guidance-for-Industry.pdf. Accessed 1 Dec 2023.
European Medicines Agency. guideline on bioanalytical method validation. 2015. Available online: https://www.ema.europa.eu/en/documents/scientific-guideline/guideline-bioanalytical-method-validation_en.pdf. Accessed 1 Dec 2023.
Bunch DR, El-Khoury JM, Gabler J, et al. Do deuterium labeled internal standards correct for matrix effects in LC-MS/MS assays? A case study using plasma free metanephrine and normetanephrine. Clin Chim Acta. 2014;15(429):4–5.
Lindegardh N, Annerberg A, White NJ, et al. Development and validation of a liquid chromatographic-tandem mass spectrometric method for determination of piperaquine in plasma stable isotope labeled internal standard does not always compensate for matrix effects. J Chromatogr B Analyt Technol Biomed Life Sci. 2008;862(1–2):227–36.
Begg EJ, Duffull SB, Hackett LP, et al. Studying drugs in human milk: time to unify the approach. J Hum Lact. 2002;18(4):323–32.
Salman S, Davis TM, Page-Sharp M, et al. Pharmacokinetics of transfer of azithromycin into the breast milk of african mothers. Antimicrob Agents Chemother. 2015;60(3):1592–9.
Damoiseaux D, Centanni D, Beijnen JH, et al. Predicting chemotherapy distribution into breast milk for breastfeeding women using a population pharmacokinetic approach. Clin Pharmacokinet. 2023;62(7):969–80.
Van Neste M, Bogaerts A, Nauwelaerts N, et al. Challenges related to acquisition of physiological data for physiologically based pharmacokinetic (PBPK) models in postpartum, lactating women and breastfed infants-a contribution from the ConcePTION project. Pharmaceutics. 2023;15(11):2618.
Giamarellou H, Kolokythas E, Petrikkos G, et al. Pharmacokinetics of three newer quinolones in pregnant and lactating women. Am J Med. 1989;87(5a):49s–51s.
Burra B, Datta P, Rewers-Felkins K, et al. Transfer of cyclobenzaprine into human milk and subsequent infant exposure. J Hum Lact. 2019;35(3):559–62.
Morganti G, Ceccarelli G, Ciaffi G. Comparative concentrations of a tetracycline antibiotic in serum and maternal milk. Antibiotica. 1968;6(3):216–23.
Weissman AM, Levy BT, Hartz AJ, et al. Pooled analysis of antidepressant levels in lactating mothers, breast milk, and nursing infants. Am J Psychiatry. 2004;161(6):1066–78.
Lopes Perdigao J, Lewey J, Hirshberg A, et al. Furosemide for accelerated recovery of blood pressure postpartum in women with a hypertensive disorder of pregnancy: a randomized controlled trial. Hypertension. 2021;77(5):1517–24.
Kristensen JH, Ilett KF, Hackett LP, et al. Gabapentin and breastfeeding: a case report. J Hum Lact. 2006;22(4):426–8.
Ostensen M, Brown ND, Chiang PK, et al. Hydroxychloroquine in human breast milk. Eur J Clin Pharmacol. 1985;28(3):357.
Ware RE, Marahatta A, Ware JL, et al. Hydroxyurea exposure in lactation: a pharmacokinetics study (HELPS). J Pediatr. 2020;222:236–9.
Lunell NO, Kulas J, Rane A. Transfer of labetalol into amniotic fluid and breast milk in lactating women. Eur J Clin Pharmacol. 1985;28(5):597–9.
Hale TW, Kristensen JH, Hackett LP, et al. Transfer of metformin into human milk. Diabetologia. 2002;45(11):1509–14.
Spigset O, Brede WR, Zahlsen K. Excretion of methylphenidate in breast milk. Am J Psychiatry. 2007;164(2):348.
Job KM, Dallmann A, Parry S, et al. Development of a generic physiologically-based pharmacokinetic model for lactation and prediction of maternal and infant exposure to ondansetron via breast milk. Clin Pharmacol Ther. 2022;111(5):1111–20.
Wojnar-Horton RE, Hackett LP, Yapp P, et al. Distribution and excretion of sumatriptan in human milk. Br J Clin Pharmacol. 1996;41(3):217–21.
Saito J, Ishii M, Mito A, et al. Trazodone levels in maternal serum, cord blood, breast milk, and neonatal serum. Breastfeed Med. 2021;16(11):922–5.
Ohman I, Vitols S, Luef G, et al. Topiramate kinetics during delivery, lactation, and in the neonate: preliminary observations. Epilepsia. 2002;43(10):1157–60.
Gilad O, Merlob P, Stahl B, et al. Outcome following tranexamic acid exposure during breastfeeding. Breastfeed Med. 2014;9(8):407–10.
Inoue H, Unno N, Ou MC, et al. Level of verapamil in human milk. Eur J Clin Pharmacol. 1984;26(5):657–8.
Verapamil. Drugs and Lactation Database (LactMed®). Bethesda (MD): National Institute of Child Health and Human Development; 2006.
Andersen HJ. Excretion of verapamil in human milk. Eur J Clin Pharmacol. 1983;25(2):279–80.
Miller MR, Withers R, Bhamra R, et al. Verapamil and breast-feeding. Eur J Clin Pharmacol. 1986;30(1):125–6.
Ilett KF, Hackett LP, Kristensen JH, et al. Transfer of dexamphetamine into breast milk during treatment for attention deficit hyperactivity disorder. Br J Clin Pharmacol. 2007;63(3):371–5.
Padro JM, Pellegrino Vidal RB, Echevarria RN, et al. Development of an ionic-liquid-based dispersive liquid-liquid microextraction method for the determination of antichagasic drugs in human breast milk: optimization by central composite design. J Sep Sci. 2015;38(9):1591–600.
Baka NE, Bayoumeu F, Boutroy MJ, et al. Colostrum morphine concentrations during postcesarean intravenous patient-controlled analgesia. Anesth Analg. 2002;94(1):184–7.
Leggett C, Lwin EMP, Ritchie U, et al. Perindopril in Breast milk and determination of breastfed infant exposure: a prospective observational study. Drug Des Devel Ther. 2020;14:961–7.
Mugwanya KK, Hendrix CW, Mugo NR, et al. Pre-exposure prophylaxis use by breastfeeding HIV-uninfected women: a prospective short-term study of antiretroviral excretion in breast milk and infant absorption. PLoS Med. 2016;13(9): e1002132.
Stowe ZN, Cohen LS, Hostetter A, et al. Paroxetine in human breast milk and nursing infants. Am J Psychiatry. 2000;157(2):185–9.
Hendrick V, Stowe ZN, Altshuler LL, et al. Fluoxetine and norfluoxetine concentrations in nursing infants and breast milk. Biol Psychiatry. 2001;50(10):775–82.
Shapiro RL, Rossi S, Ogwu A, et al. Therapeutic levels of lopinavir in late pregnancy and abacavir passage into breast milk in the Mma Bana Study, Botswana. Antivir Ther. 2013;18(4):585–90.
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This work is partially funded by grants from the Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD, Grant no. HD047905 and Grant no. HD096796).
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Osama Y. Alshogran, Prerna Dodeja, Hamdan Albukhaytan, Taylor Laffey, Nupur Chaphekar, Steve Caritis, Imam H. Shaik, and Raman Venkataramanan declare that they have no potential conflicts of interest that might be relevant to the contents of this manuscript.
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O.A., P.D., H.A., I.S., and R.V. participated in conceptualization; O.A., P.D., H.A., T.L., and I.S. conducted experiments/ data mining; O.A., P.D., and H.A. performed data analysis; O.A., P.D., H.A., N.K., and I.S. prepared figures and tables; and O.A., P.D., H.A., T.L., S.C., I.S., and R.V. wrote/contributed to the manuscript. All authors read and approved the final version of the manuscript.
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Alshogran, O.Y., Dodeja, P., Albukhaytan, H. et al. Drugs in Human Milk Part 1: Practical and Analytical Considerations in Measuring Drugs and Metabolites in Human Milk. Clin Pharmacokinet 63, 561–588 (2024). https://doi.org/10.1007/s40262-024-01374-3
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DOI: https://doi.org/10.1007/s40262-024-01374-3