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NADPH oxidase 4-mediating oxidative stress contributes to endometriosis

  • Human Genetics • Original Paper
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Abstract

NADPH oxidase 4 (NOX4) plays an important role in the regulation of oxidative stress, which is associated with endometriosis. This study aims to investigate the effects of NOX4 in endometriosis and its molecular mechanisms. Clinical specimens were collected, and human endometrial stromal cells (HESCs) were isolated. The knockdown of NOX4 cell lines was established on the HESCs and induced by peritoneal fluid. The levels of NOX4 were determined by using immunohistochemistry (IHC) staining, western blotting, and qPCR, respectively. The levels of oxidative stress markers were determined by using western blotting and ELISAs, respectively. The correlation of NOX4 and oxidative stress markers was analyzed by the Pearson correlation coefficient. The levels of NOX4 were dramatically elevated in the ectopic endometrium. Besides, oxidative stress biomarkers were also dysregulated in the ectopic endometrium as compared to the normal endometrium. Pearson’s correlation coefficient analysis revealed a relationship between NOX4 and oxidative stress biomarkers in the ectopic endometrium. NOX4 modulated the expressions of oxidative stress markers in endometrial stromal cells stimulated by the peritoneal fluid from endometriosis. The effects of NOX4 on endometriosis are in part by its regulatory effects against oxidative stress.

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The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

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Author notes

  1. Xiaojie Wang, Xiaona Jiang, and Xin Lv contributed equally to this work.

Authors and Affiliations

  1. Department of Gynecology, the Affiliated Yantai Yuhuangding Hospital of Qingdao University, No.20 Yuhuangding East Road, Yantai, 264000, Shandong, China

    Xiaojie Wang, Xiaona Jiang, Xin Lv, Xinshu Wang, Aimin Lin & Yangyang Li

Authors
  1. Xiaojie Wang
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  2. Xiaona Jiang
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  3. Xin Lv
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  4. Xinshu Wang
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  5. Aimin Lin
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  6. Yangyang Li
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Contributions

All authors contributed to the study’s conception and design. Material preparation, data collection, and analysis were performed by Xiaojie Wang, Xiaona Jiang, Xin Lv, Xinshu Wang, and Aimin Lin. The first draft of the manuscript was written by Yangyang Li. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Yangyang Li.

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Ethics approval

The study was approved by the Affiliated Yantai Yuhuangding Hospital of Qingdao University, and written informed consent was derived from each participant, the approval number is 2013–90. The study was performed in strict accordance with the Declaration of Helsinki, Ethical Principles for Medical Research Involving Human Subjects.

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Current study is available from the corresponding author on reasonable request.

Conflict of interest statement

The authors declare no competing interests.

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Communicated by Ewa Ziętkiewicz

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Wang, X., Jiang, X., Lv, X. et al. NADPH oxidase 4-mediating oxidative stress contributes to endometriosis. J Appl Genetics 65, 113–120 (2024). https://doi.org/10.1007/s13353-023-00810-7

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  • DOI: https://doi.org/10.1007/s13353-023-00810-7

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