We studied the effects of age and suntan on the expression of necroptosis signaling molecules (RIPK1, RIPK3, and MLKL kinases) and first TNF receptor (TNFR1) in isolated skin cells from women undergoing facelift surgery. In women above 50 years, the expression of the TNFR1, kinases RIPK1, RIPK3, and MLKL, the phosphorylated forms of these kinases was significantly (p<0.05) increased in comparison with the corresponding parameters in women under 30 years. The expression of all necroptosis proteins and TNFR1 in women with suntan was significantly (p<0.05) higher than in those without tan. Cells from the surgical material were incubated with TNFα to determine the level of induced necroptosis. In women aged >50 years and women with suntan, the expression of phosphorylated forms of kinases was significantly increased, which attested to necroptosis activation. This study allowed identifying the targets on skin cells for prevention of necrosis and inflammation after facelift surgery.
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Park MY, Ha SE, Vetrivel P, Kim HH, Bhosale PB, Abu-saliya A, Kim GS. Differences of key proteins between apoptosis and necroptosis. Biomed. Res. Int. 2021;2021:3420168. https://doi.org/10.1155/2021/3420168
Meng Y, Davies KA, Fitzgibbon C, Young SN, Garnish SE, Horne CR, Luo C, Garnier JM, Liang LY, Cowan AD, Samson AL, Lessene G, Sandow JJ, Czabotar PE, Murphy JM. Human RIPK3 maintains MLKL in an inactive conformation prior to cell death by necroptosis. Nat. Commun. 2021;12(1):6783. https://doi.org/10.1038/s41467-021-27032-x
Frank D, Vaux DL, Murphy JM, Vince JE, Lindqvist LM. Activated MLKL attenuates autophagy following its translocation to intracellular membranes. J. Cell Sci. 2019;132(5):jcs220996. https://doi.org/10.1242/jcs.220996
Liang W, Qi W, Geng Y, Wang L, Zhao J, Zhu K, Wu G, Zhang Z, Pan H, Qian L, Yuan J. Necroptosis activates UPR sensors without disrupting their binding with GRP78. Proc. Natl Acad. Sci. USA. 2021;118(39):e2110476118. https://doi.org/10.1073/pnas.2110476118
Shlomovitz I, Erlich Z, Arad G, Edry-Botzer L, Zargarian S, Cohen H, Manko T, Ofir-Birin Y, Cooks T, Regev-Rudzki N, Gerlic M. Proteomic analysis of necroptotic extracellular vesicles. Cell Death Dis. 2021;12(11):1059. https://doi.org/10.1038/s41419-021-04317-z
Tang Z, Tong X, Huang J, Liu L, Wang D, Yang S. Research progress of keratinocyte-programmed cell death in UV-induced Skin photodamage. Photodermatol. Photoimmunol. Photomed. 2021;37(5):442-448. https://doi.org/10.1111/phpp.12679
Yu Z, Efstathiou NE, Correa VSMC, Chen X, Ishihara K, Iesato Y, Narimatsu T, Ntentakis D, Chen Y, Vavvas DG. Receptor interacting protein 3 kinase, not 1 kinase, through MLKL-mediated necroptosis is involved in UVA-induced corneal endothelium cell death. Cell Death Discov. 2021;7(1):366. https://doi.org/10.1038/s41420-021-00757-w
Alafiatayo AA, Lai KS, Ahmad S, Mahmood M, Shaharuddin NA. RNA-Seq analysis revealed genes associated with UV-induced cell necrosis through MAPK/TNF-α pathways in human dermal fibroblast cells as an inducer of premature photoaging. Genomics. 2020;112(1):484-493. https://doi.org/10.1016/j.ygeno.2019.03.011
Lee KJ, Park KH, Hahn JH. Alleviation of ultraviolet-B radiation-induced photoaging by a TNFR antagonistic peptide, TNFR2-SKE. Mol. Cells. 2019;42(2):151-160. https://doi.org/10.14348/molcells.2018.0423
Rius-Pérez S, Pérez S, Toledano MB, Sastre J. p53 drives necroptosis via downregulation of sulfiredoxin and peroxiredoxin 3. Redox Biol. 2022;56:102423. https://doi.org/10.1016/j.redox.2022.102423
Molnár T, Pallagi P, Tél B, Király R, Csoma E, Jenei V, Varga Z, Gogolák P, Odile Hueber A, Máté Z, Erdélyi F, Szabó G, Pettkó-Szandtner A, Bácsi A, Virág L, Maléth J, Koncz G. Caspase-9 acts as a regulator of necroptotic cell death. FEBS J. 2021;288(22):6476-6491. https://doi.org/10.1111/febs.15898
Li X, Zhong CQ, Wu R, Xu X, Yang ZH, Cai S, Wu X, Chen X, Yin Z, He Q, Li D, Xu F, Yan Y, Qi H, Xie C, Shuai J., Han J. RIP1-dependent linear and nonlinear recruitments of caspase-8 and RIP3 respectively to necrosome specify distinct cell death outcomes. Protein Cell. 2021;12(11):858-876. https://doi.org/10.1007/s13238-020-00810-x
Pilkington SM, Ogden S, Eaton LH, Dearman RJ, Kimber I, Griffiths CEM. Lower levels of interleukin-1β gene expression are associated with impaired Langerhans’ cell migration in aged human skin. Immunology. 2018;153(1):60-70. https://doi.org/10.1111/imm.12810
Abramian ShM, Morozov SG. Spontaneous and induced apoptosis of cells obtained from surgical materials after face lift. Patogenez. 2016;14(4): 63-68. Russian.
Abramyan ShM, Blokhin SN, Morozov SG. The role of cellular energetics in the arrest of facial tissue necrosis. Vestn. Ross. Voen. Med. Akad. 2017;(4, Suppl. 1):97. Russian.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 174, No. 12, pp. 674-677, December, 2022
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Abramyan, S., Volkova, E.N. & Morozov, S.G. Effects of Age and Suntan on the Expression of Second Messenger Signaling Pathways of Necroptosis in Skin Cells during Facelifting Surgery. Bull Exp Biol Med 174, 707–710 (2023). https://doi.org/10.1007/s10517-023-05775-5
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DOI: https://doi.org/10.1007/s10517-023-05775-5