Abstract
The human body is almost always facing the oxidative stress caused by foodborne aldehydes such as glyoxal (GO) and methylglyoxal (MGO), 4-hydroxyhexenal (HHE), and 4-hydroxynonenal (HNE). When these aldehydes build up, they can cause a range of harm. However, a probiotic, Clostridium butyricum, can increase nuclear factor erythroid-2 related factor 2 (Nrf2) and may have the potential to relieve oxidative stress. If C. butyricum is indeed resistant to aldehydes, the advantages (accessibility, convenience, and safety) will be of great significance compared with drugs. Unfortunately, whether C. butyricum can play a role in alleviating toxic effects of foodborne aldehydes in the intestine (the first line of defense against food-derived toxin) was unclear. To investigate these, we measured the viability, ROS, autophagy, and inflammatory cytokine expression of Caco-2 which were co-cultured with C. butyricum and stimulated by the four aldehydes via Nrf2 pathway (Staphylococcus aureus and Enterococcus faecium as controls). Then, we explored the link among C. butyricum, NLRP6, and Nrf2 signaling pathways when facing the stimuli. In the present study, we demonstrated that Clostridium butyricum relieved the oxidative stress induced by the aldehydes in Caco-2. Most interestingly, we found a “complementary” relationship between NLRP6 and Nrf2 in C. butyricum treatment under aldehyde stress. Our research not only makes a contribution to the popularization of C. butyricum as a probiotic-rich food instead of medicines but also sheds new light on the application of subsequent microecological formulation of C. butyricum.
Key points
• The adverse effects are caused in a dose-dependent manner by foodborne aldehydes.
• Clostridium butyricum can significantly ameliorate oxidative stress.
• There is a “complementary” relationship between the NLRP6 and Nrf2 signaling pathways.
• Using Clostridium butyricum foods to alleviate oxidative stress shows great prospects.
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Funding
The study was funded by the Natural Science Foundation of Guangdong Province (2015A030312005), Guangzhou Science and Technology Project (202002020056, 201903010078), Lingnan Modern Agriculture Key Project (mmkj2020026), and Natural Science Foundation of Guangdong Province (2018A030313625).
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J.Y.L. developed the search strategy. D.C. and M.Z. assisted with the experiments. H.M.C., J.T.W., Q.F., F.P.M., M.P.M., Q.Y.L., J.H.L., and S.X.Z. contributed to summarizing and analyzing the data. H.K.S. and Z.J.Z. contributed to editing and revising the manuscript. L.H.Z. conceived the idea for the study and revised the manuscript. All authors read and approved the final manuscript.
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Li, J., Shen, H., Zhao, Z. et al. Protective effects of Clostridium butyricum against oxidative stress induced by food processing and lipid-derived aldehydes in Caco-2 cells. Appl Microbiol Biotechnol 104, 9343–9361 (2020). https://doi.org/10.1007/s00253-020-10896-2
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DOI: https://doi.org/10.1007/s00253-020-10896-2