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Hesperidin neuroprotective effects against carbon monoxide-induced toxicity in male rats

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Abstract

Carbon monoxide (CO) is produced via incomplete combustion of fossil fuels and it may cause long-term neurological sequel upon exposure. Hesperidin (HES), a flavanone glycoside found in citrus plants, exerts diverse beneficial health effects. The present study mechanistically examined the neuroprotective effects of HES in CO-poisoned rats. Thirty male Wistar rats (five groups of six animals) were exposed to 3000 ppm CO for 1 h. Immediately after the exposure and on the next 4 consecutive days (totally five doses), rats intraperitoneally received either normal saline (the control group) or different doses of HES (25, 50, and 100 mg/kg). A sham group that was not exposed to CO was also considered. After evaluation of spatial learning and memory using a Morris water maze (MWM), animals were sacrificed and oxidative stress status in blood samples, and Akt, Bax, Bcl2, and brain-derived neurotrophic factor (BDNF) expression in brain samples were assessed. Western blot analysis indicated increased Akt but decreased Bax/Bcl2 levels in the HES 100 mg/kg, and induced BDNF levels in all HES-treated groups. MWM results showed that HES significantly decreased memory loss. The current findings indicate that HES could alleviate neurological impairments induced by CO in rats.

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Acknowledgements

The authors would like to thank the vice chancellor for research, Zabol University of Medical Sciences, Zabol, Iran for the financial support of the present work.

Funding

This study was supported by the vice chancellor for research at Zabol University of Medical Sciences, Zabol, Iran.

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Author notes

  1. Jafar Shahraki and Kaveh Tabrizian contributed equally to this work.

Authors and Affiliations

  1. Department of Pharmacodynamics and Toxicology, School of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran

    Jafar Shahraki, Kaveh Tabrizian, Behnam Tashakori, Zahra Dadrezaei & Mahmoud Hashemzaei

  2. Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Ramin Rezaee & Vahideh Ghorani

  3. Clinical Research Development Unit, Faculty of Medicine, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran

    Vahideh Ghorani

  4. Department of Health, Zabol University of Medical Sciences, Zabol, Iran

    Gholamreza Bagheri

  5. Department of Pathology, Amiralmomenin Hospital, Zabol University of Medical Sciences, Zabol, Iran

    Hosseinali Jahantigh

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  1. Jafar Shahraki
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Contributions

JSH and KT performed experimental work, drafted the manuscript, designed the figures, and compiled the literature sources. BT and ZD have processed the experimental data and performed the analysis. VGH, GHB and HJ have contributed in implementation of the research, to the interpretation of the results, and the writing of the manuscript. MH and RR has contributed to the design, supervising of the experimental work, help in first version manuscript drafting, and help in manuscript revising. All Authors read and approved the final version of the manuscript. The authors declare that all data were generated in-house and that no paper mill was used.

Corresponding authors

Correspondence to Ramin Rezaee or Mahmoud Hashemzaei.

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This study was approved by the Animal Research Ethics Committee of Zabol University of Medical Sciences (Ethics Committee approval No. IR.ZBMU.REC.1399.031) and carried out according to the guidelines of the National Institute of Health Guide for the Care and Use of the Laboratory Animals.

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Shahraki, J., Tabrizian, K., Rezaee, R. et al. Hesperidin neuroprotective effects against carbon monoxide-induced toxicity in male rats. Naunyn-Schmiedeberg's Arch Pharmacol (2024). https://doi.org/10.1007/s00210-024-03132-5

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