Snakemake pipeline for scRNA-seq gene expression data analysis in biowulf. Starts from cellRanger output.
Inputs:
- Folder containing CellRanger outputs h5 files (cellrangerOut under working directory)
- groups.tab with 4 columns: Output directory, group ID, sample alias, data type
- contrasts.tab with group names tab seperated
Outputs:
- Each sample produces the filtered sample RDS object
- Integrated seurat objects from three methods: CCA, RPCA and harmony, as well as merged (not batch corrected) seurat object. Samples for integration are chosen based on contrasts.tab