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Snakemake pipeline for scRNA-seq gene expression data analysis in biowulf. Starts from cellRanger output.

Inputs:

  1. Folder containing CellRanger outputs h5 files (cellrangerOut under working directory)
  2. groups.tab with 4 columns: Output directory, group ID, sample alias, data type
  3. contrasts.tab with group names tab seperated

Outputs:

  1. Each sample produces the filtered sample RDS object
  2. Integrated seurat objects from three methods: CCA, RPCA and harmony, as well as merged (not batch corrected) seurat object. Samples for integration are chosen based on contrasts.tab

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