Sebastien Ladet, Ph.D.

Aix-en-Provence, Provence-Alpes-Côte d’Azur, France Coordonnées
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Activité

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Publications

  • Large pore size and controlled mesh elongation are relevant predictors for mesh integration quality and low shrinkage

    International Journal of Surgery

    Prosthetic mesh implants in hernia repair are frequently used based on the fact that lower recurrence rates are detected. However, an undesirable side effect is persistent foreign body reaction that drives adhesions and shrinkage among other things in the course of time. Thereby a variety of meshes have been created in an attempt to alleviate these side effects, and particular relating to shrinkage, the ideal mesh has not been developed. Large pore size is one of the properties to get better…

    Prosthetic mesh implants in hernia repair are frequently used based on the fact that lower recurrence rates are detected. However, an undesirable side effect is persistent foreign body reaction that drives adhesions and shrinkage among other things in the course of time. Thereby a variety of meshes have been created in an attempt to alleviate these side effects, and particular relating to shrinkage, the ideal mesh has not been developed. Large pore size is one of the properties to get better ingrowth of the implants but could also be a risk factor to shrinkage behavior. The aim of this preclinical study was to determine optimal pore size based on mesh integration and shrinkage in a hernia minipig model

    Other authors
    • Dirk Weyhe
    • William Cobbb,
    • Julie Lecuivre
    • Antoine Alves
    • Davide Lomanto
    • Yves Bayon
    See publication

  • Multi-membrane chitosan hydrogels as chondrocytic cell bioreactors

    Biomaterials

    We investigated the bioactivity of new chitosan-based multi-membrane hydrogel (MMH) architectures towards chondrocyte-like cells. The microstructure of the hydrogels constituting the membranes precludes any living cell penetration, whereas their lower scale architecture allows the protein diffusion. The biological behavior of chondrocytes implanted within the MMH inter-membrane spaces was studied for 45 days in culture. Chondrocytes formed cell aggregates and proliferated without loosing their…

    We investigated the bioactivity of new chitosan-based multi-membrane hydrogel (MMH) architectures towards chondrocyte-like cells. The microstructure of the hydrogels constituting the membranes precludes any living cell penetration, whereas their lower scale architecture allows the protein diffusion. The biological behavior of chondrocytes implanted within the MMH inter-membrane spaces was studied for 45 days in culture. Chondrocytes formed cell aggregates and proliferated without loosing their chondrogenic phenotype as illustrated by collagen II and aggrecan expressions at the mRNA and protein levels. Cells produced neo-formed alcyan blue matrix proteins filling MMH interspaces. The HiF-2a/ SOX9 pattern of expression suggested that the elevated chondrocytic phenotype in MMH could be related to a better hypoxic local environment than in classical culture conditions. Pro-inflammatory markers were not expressed during the period of culture. The low level of nitric oxide accumulation within the inter-membrane spaces and in the incubation medium implied that chitosan consumed nitrites produced by entrapped chondrocytes, in relation with the decrease of its molecular weight of 50%. Our data suggest that MMH structures may be considered as complex chondrocytic cell bioreactors; “active decoys of biological media”, potentially promising for various biomedical applications like the inter-vertebral disk replacement.

    Other authors
    • maite corvol
    See publication

  • Multi-membrane hydrogels

    Nature

    Polysaccharide-based hydrogels are useful for numerous applications, from food1 and cosmetic processing to drug delivery and tissue engineering2, 3. The formation of hydrogels from polyelectrolyte solutions is complex, involving a variety of molecular interactions. The physical gelation of polysaccharides can be achieved by balancing solvophobic and solvophilic interactions4. Polymer chain reorganization can be obtained by solvent exchange, one of the processing routes forming a simple hydrogel…

    Polysaccharide-based hydrogels are useful for numerous applications, from food1 and cosmetic processing to drug delivery and tissue engineering2, 3. The formation of hydrogels from polyelectrolyte solutions is complex, involving a variety of molecular interactions. The physical gelation of polysaccharides can be achieved by balancing solvophobic and solvophilic interactions4. Polymer chain reorganization can be obtained by solvent exchange, one of the processing routes forming a simple hydrogel assembly. Nevertheless, many studies on hydrogel formation are empirical with a limited understanding of the mechanisms involved, delaying the processing of more complex structures. Here we use a multi-step interrupted gelation process in controlled physico-chemical conditions to generate complex hydrogels with multi-membrane ‘onion-like’ architectures. Our approach greatly simplifies the processing of gels with complex shapes and a multi-membrane organization. In contrast with existing assemblies described in the literature, our method allows the formation of free ‘inter-membrane’ spaces well suited for cell or drug introduction. These architectures, potentially useful in biomedical applications, open interesting perspectives by taking advantage of tailor-made three-dimensional multi-membrane tubular or spherical structures.

    Other authors
    • alain domard
    • laurent david
    See publication

Brevets

  • FUNCTIONALIZED SURGICAL ADHESIVES

    Émis le US US20090154378P

    A bioadherent composition includes a first mixture containing a plurality of reactive members of a specific binding pair, said reactive members being bound to a ligand capable of binding a receptor on biological tissue, and a second mixture containing a plurality of complementary reactive members of the specific binding pair, said complementary reactive members being bound to a ligand capable of binding a receptor on biological tissue, said reactive members capable of forming covalent bonds…

    A bioadherent composition includes a first mixture containing a plurality of reactive members of a specific binding pair, said reactive members being bound to a ligand capable of binding a receptor on biological tissue, and a second mixture containing a plurality of complementary reactive members of the specific binding pair, said complementary reactive members being bound to a ligand capable of binding a receptor on biological tissue, said reactive members capable of forming covalent bonds with said complementary reactive members via a reaction selected from Huisgen cycloaddition reactions, Diels-Alder reactions, and/or thiol-alkene reactions. A method for bonding biological tissue involves utilizing the bioadherent composition.

    See patent

  • SELF-ASSEMBLING FIBROUS NETWORKS

    Émis le US US20090154372P

    The present invention relates to a kit comprising : - a plurality of fibers which have a plurality of reactive members of a primary specific binding pair attached to a surface of the fibers, and - a container adapted to contain a mixture including complementary reactive members of the primary specific binding pair, the complementary reactive members having a functionality that will adhere them to biological tissue upon contact.

    See patent

  • MEDICAL DEVICE WITH DEGRADATION-RETARDING COATING

    Émis le US WO2010095044

    The present disclosure relates to implantable bioabsorbable medical devices having an amphiphilic coating for delaying the degradation of the device and methods of making such devices.

    See patent

  • MEDICAL DEVICES INCORPORATING FUNCTIONAL ADHESIVES

    Émis le US US20090154367P

    The invention relates to a kit comprising: - a polymeric medical device having a plurality of reactive members of a specific binding pair attached to a surface of the device; and a container containing a mixture including complementary reactive members of the specific binding pair, the complementary reactive members having a functionality adapted to adhere them to biological tissue upon contact.

    See patent

  • FUNCTIONALIZED ADHESIVE MEDICAL GEL

    Émis le US US20090154369P

    The present invention relates to a kit comprising: - a medical gel having a plurality of reactive members of a specific binding pair adapted to be attached on a surface of the gel; and - a container containing a mixture including complementary reactive members of the specific binding pair, the complementary reactive members having a functionality that will adhere them to biological tissue upon contact. The invention further relates to a bioadherent substrate including such a medical gel and to…

    The present invention relates to a kit comprising: - a medical gel having a plurality of reactive members of a specific binding pair adapted to be attached on a surface of the gel; and - a container containing a mixture including complementary reactive members of the specific binding pair, the complementary reactive members having a functionality that will adhere them to biological tissue upon contact. The invention further relates to a bioadherent substrate including such a medical gel and to a method of forming such a medical gel.

    See patent

  • MEDICAL DEVICES WITH DEFINABLE POROSITY PRODUCED BY BACTERIAL POLYMER BIO-SYNTHESIS

    Émis le US US20080112295P

    The present invention relates to a method of forming an implantable medical device comprising: culturing polymer-producing bacteria preferably Acetobacter xylinum in the presence of a degradable support; and removing the degradable support to recover an implant having pores of a configuration determined at least in part by the configuration of the degradable support. The invention also relates to a medical device obtained by such a method.

    See patent

  • AUTO-SEALANT MATRIX FOR TISSUE REPAIR

    Émis le US US20080196544P

    Implants for tissue repair/regeneration include a porous layer combined with dry materials that are activated to form a hydrogel upon contact with aqueous physiological fluids.

    See patent

  • SURGICAL PATCH

    Émis le US US20080106354P

    See patent

  • MATRIX FOR TISSUE REPAIR

    Émis le US US20080196487P

    Implants include a porous layer made from a composition including a compound wherein collagen is cross-linked to a glycosaminoglycan, the porous layer being optionally joined to a collagen film.

    See patent

  • Bioresorbable and biocompatible compounds for surgical use

    Émis le US US20070899694

    A bioresorbable and biocompatible compound for surgical use is composed of functionalized collagen cross-linked with a glycosaminoglycan.

    See patent

  • DURAL REPAIR MATERIAL

    Émis le US WO2009022230

    Multilayer structures including a porous layer and a non-porous layer having a reinforcement member are useful as dural repair materials.

    See patent

  • MULTIPLE-MEMBRANE POLYSACCHARIDE CAPSULES

    Émis le FR WO2006092540

    The invention concerns a method for preparing multiple-membrane capsules formed by a series of non-integral membranes and consisting of a common ionizable polysaccharide, in the form of a physical hydrogel, including the following steps: a) forming an ionizable polysaccharide physical hydrogel, in cationic or anionic form, in a mold with shape adapted to the shape desired for the capsule; b) immersing the hydrogel into an alkali solution in the case of a cationic polysaccharide, or an acid…

    The invention concerns a method for preparing multiple-membrane capsules formed by a series of non-integral membranes and consisting of a common ionizable polysaccharide, in the form of a physical hydrogel, including the following steps: a) forming an ionizable polysaccharide physical hydrogel, in cationic or anionic form, in a mold with shape adapted to the shape desired for the capsule; b) immersing the hydrogel into an alkali solution in the case of a cationic polysaccharide, or an acid solution in the case of an anionic polysaccharide, for a given immersion time, then allowing it to rest outside the solution for a given resting time, so as to obtain a first membrane; c) repeating step b, as many times as the desired number of additional membranes, to form successive membranes from outside inwards of the capsule.

    See patent

Prix et distinctions

  • President's award Surgical Devices - Covidien: 2010

    Surgical Devices

  • Braconnot Prize

    European Chitin Society

    The Braconnot Prize is awarded for outstanding contributions and achievements in chitin science and for special merits in the promotion of EUCHIS. - See more at: http://euchis.org/#sthash.sAcpyJI0.dpuf

    http://euchis.org/previous-winners-of-the-braconnot-prize/

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