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Grape skin extract-derived polyphenols modify programming-induced renal endowment in prenatal protein-restricted male mouse offspring

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Abstract

Purpose

Protein-restricted diet during pregnancy is related to oxidative stress and, as a consequence, damage to nephrogenesis. We investigated the effects of vinifera grape skin extract (ACH09)-derived polyphenols on preserving renal morphology of maternal protein-restricted 1-day-old offspring.

Methods

Female C57/Bl-6 mice were fed two different isocaloric diets: control diet (19.3 % protein) and low-protein diet (6 % protein) with access to water or to the extract dissolved in drinking water (19.3 % protein plus ACH09 200 mg kg−1 day−1 and 6 % protein plus ACH09 200 mg kg−1 day−1) throughout gestation. Renal morphology—glomerular number N[glom]; renal maturity—vascular glomeruli and avascular glomeruli ratio (v–N[glom]/a-N[glom]); medullar and cortical volumes, as well as mean glomerular volume, were analyzed in male offspring. Hepatic superoxide dismutase and catalase (CAT) activities were evaluated, and renal lipid peroxidation levels were measured.

Results

Maternal protein restriction affected birth weight and naso-anal length in low-protein offspring compared to control and ACH09 restored both parameters. Protein restriction increased lipid peroxidation in kidney and liver and reduced CAT activity in low-protein group compared to control. Supplementation with ACH09 reduced the kidney oxidative damage and restored the antioxidant activity of CAT. ACH09 prevented glomerular loss and renal immaturity in the offspring.

Conclusion

The treatment of low-protein-fed dams during pregnancy with ACH09 provides protection from early-life deleterious renal morphological changes. The protective effect of ACH09 may involve antioxidant action and vasodilator effect of the extract.

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Acknowledgments

This work was supported by National Council of Scientific and Technological Development (CNPq, Protocol 473514/2011-7) and Rio de Janeiro State Research Agency (FAPERJ; Protocol E-26/102.920/2011).

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Authors and Affiliations

  1. Department of Pharmacology, Rio de Janeiro State University, Av. 28 de Setembro, nº 87, Rio de Janeiro, CEP: 20551-030, Brazil

    Mariana Ribeiro Costa, Mariane Nogueira Nalbones-Barbosa, Ângela Castro Resende & Roberto Soares de Moura

  2. Biomedical Sciences Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

    Karla Maria Pereira Pires & Samuel dos Santos Valença

Authors
  1. Mariana Ribeiro Costa
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  2. Karla Maria Pereira Pires
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  3. Mariane Nogueira Nalbones-Barbosa
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  4. Samuel dos Santos Valença
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  5. Ângela Castro Resende
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  6. Roberto Soares de Moura
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Corresponding author

Correspondence to Ângela Castro Resende.

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Conflict of interest

Roberto Soares de Moura is the inventor of a patent (PCT/BR02/00038) that supported the development of a new patent application (PI0605693 A2-8). The other authors state no conflicts of interest.

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Costa, M.R., Pires, K.M.P., Nalbones-Barbosa, M.N. et al. Grape skin extract-derived polyphenols modify programming-induced renal endowment in prenatal protein-restricted male mouse offspring. Eur J Nutr 55, 1455–1464 (2016). https://doi.org/10.1007/s00394-015-0963-5

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  • DOI: https://doi.org/10.1007/s00394-015-0963-5

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