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Effect of Prebiotic Galacto-Oligosaccharides on Serum Lipid Profile of Hypercholesterolemics

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Abstract

The present study focused on the role of galacto-oligosaccharides (GOS) against the development of hypercholesterolemia. In the current research, GOS synthesized from lactose solution were fed to hypercholesterolemic female Sprague–Dawley rats. Negative control group (G1) was fed on standard basal diet alone. Positive control group (G5) was fed on inulin (154 mg/250 g body weight), while treatment groups G2, G3 and G4 received 110 mg, 154 mg and 198.4 mg/250 g body weight, respectively, of GOS along with high-fat diet for a period of 60 days. Findings from this study revealed that animals belonging to prebiotic GOS (G2, G3 and G4)-fed group showed significantly decreased serum triglycerides, total cholesterol, LDL cholesterol and VLDL cholesterol as compared to control group (G0). The groups which were fed on different doses of GOS revealed a significant reduction in TC, TG, LDL, and VLDL levels and an increase in HDL level corresponding to the reference group that was fed on inulin, while G1 negative control group revealed increased levels of TC, TG, LDL and VLDL. In contrast to positive control group G5 (154 mg inulin), all doses of GOS lowered serum TC, TG and LDL-C and raised HDL-C; however, G4 (198.4 mg) proved to be more effective. Hence, GOS proved to be supportive in preventing hypercholesterolemia leading cause of cardiovascular disease and atherosclerosis. This study reported a significant reduction of serum TC, TG and LDL-C in female rats for 60 days as compared to control. Conclusively, GOS were found to be worthless against hypercholesterolemia.

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Acknowledgments

We would like to express our heartfelt gratitude to all those who gave us the possibility to complete this project. We are deeply indebted to Dr. Sanaullah Iqbal, Department of Food Science and Human Nutrition, UVAS, whose help, stimulating suggestions, knowledge, experience and encouragement helped us in all the times of study and analysis of the project in the pre- and post-research period. Also, a special thanks to the staff of laboratories of Department of Food Science and Human Nutrition, University of Veterinary And Animal Sciences, Lahore, for fulfilling each and every requirement, bearing with us and their constant support.

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Authors and Affiliations

  1. Department of Food Science and Human Nutrition, University of Veterinary and Animal Sciences, Lahore, Pakistan

    Arooj Hashmi, Naureen Naeem, Zubair Farooq & Sanaullah Iqbal

  2. Department of Anatomy and Histology, University of Veterinary and Animal Sciences, Lahore, Pakistan

    Saima Masood

  3. Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan

    Rahat Naseer

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  1. Arooj Hashmi
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Correspondence to Naureen Naeem.

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It is stated that the above-mentioned article to be submitted in the Probiotics and Antimicrobial Proteins is original, has been written by the stated authors and has not been published elsewhere. And all the authors contributed significantly in research work till the completion of this manuscript. It is declared that the article was not submitted for review to another journal while under review by this journal and will not be submitted to any other journal. There is no conflict of interest between the coauthors. I have informed the coauthor(s) of the terms of this License Agreement and that I am submitting on their behalf, and I am authorized to do so.

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Hashmi, A., Naeem, N., Farooq, Z. et al. Effect of Prebiotic Galacto-Oligosaccharides on Serum Lipid Profile of Hypercholesterolemics. Probiotics & Antimicro. Prot. 8, 19–30 (2016). https://doi.org/10.1007/s12602-016-9206-1

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