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Physiology and glomerular projections of olfactory receptor neurons on the antenna of female Heliothis virescens (Lepidoptera: Noctuidae) responsive to behaviorally relevant odors

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Abstract

The neurophysiology and antennal lobe projections of olfactory receptor neurons housed within short trichoid sensilla of female Heliothis virescens F. (Noctuidae: Lepidoptera) were investigated using a combination of cut-sensillum recording and cobalt-lysine staining techniques. Behaviorally relevant odorants, including intra- and inter-sexual pheromonal compounds, plant and floral volatiles were selected for testing sensillar responses. A total of 184 sensilla were categorized into 25 possible sensillar types based on odor responses and sensitivity. Sensilla exhibited both narrow (responding to few odors) and broad (responding to many odors) response spectra. Sixty-six percent of the sensilla identified were stimulated by conspecific odors; in particular, major components of the male H. virescens hairpencil pheromone (hexadecanyl acetate and octadecanyl acetate) and a minor component of the female sex pheromone, (Z)-9-tetradecenal. Following characterization of the responses, olfactory receptor neurons within individual sensilla were stained with cobalt lysine (N=39) and traced to individual glomeruli in the antennal lobe. Olfactory receptor neurons with specific responses to (Z)-9-tetradecenal, a female H. virescens sex pheromone component, projected to the female-specific central large female glomerulus (cLFG) and other glomeruli. Terminal arborizations from sensillar types containing olfactory receptor neurons sensitive to male hairpencil components and plant volatiles were also localized to distinct glomerular locations. This information provides insight into the representation of behaviorally relevant odorants in the female moth olfactory system.

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Abbreviations

Z11-16:Ald:

(Z)-11-hexadecenal

Z9-14:Ald:

(Z)-9-tetradecenal

Z9-16:Ald:

(Z)-9-hexadecenal

Z11-16:OAc:

(Z)-11-hexadecenyl acetate

Z11-16:OH:

(Z)-11-hexadecen-1-ol

14:OOH:

tetradecanoic acid

16:OH:

hexadecanol

18:OH:

octadecanol

16:OAc:

hexadecanyl acetate

18:OAc:

octadecanyl acetate

16:OOH:

hexadecanoic acid

18:OOH:

octadecanoic acid

Z3-6:OH:

(Z)-3-hexen-1-ol

Z3-6:OAc:

(Z)-3-hexenyl acetate

E2-6:Ald:

(E)-2-hexenal

ORN:

olfactory receptor neuron

PN:

projection neuron

AL:

antennal lobe

MGC:

macroglomerular complex

LFG:

large female glomeruli

cLFG:

central large female glomerulus

mLFG:

medial large female glomerulus

EAG:

electroantennogram

SSR:

single sensillum recording

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Acknowledgements

Special thanks to J. Celestino, K. Iceman and D. Kelly for colony maintenance and to C. Fogarty for valuable comments on the manuscript. We are also grateful to Dr. R. Raguso and Dr. J. Tumlinson for kindly providing volatile stock solutions. We also thank S.G. Lee and Dr. T.C. Baker for advice with the cobalt-lysine staining technique. This research was funded by USDA NRICGP no. 1999-03541 and NSF-IBN 9905683 to NJV.

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  1. Department of Biology, University of Utah, Room 201 South Biology, Salt Lake City, UT, 84112, USA

    N. K. Hillier & N. J. Vickers

  2. Department of Behavioral Physiology and Sociobiology, Zoologie II, Biozentrum, Am Hubland, 97074, Würzburg, Germany

    C. Kleineidam

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  1. N. K. Hillier
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  2. C. Kleineidam
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  3. N. J. Vickers
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Correspondence to N. K. Hillier.

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Hillier, N.K., Kleineidam, C. & Vickers, N.J. Physiology and glomerular projections of olfactory receptor neurons on the antenna of female Heliothis virescens (Lepidoptera: Noctuidae) responsive to behaviorally relevant odors. J Comp Physiol A 192, 199–219 (2006). https://doi.org/10.1007/s00359-005-0061-x

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  • DOI: https://doi.org/10.1007/s00359-005-0061-x

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