Skip to main content
Log in

Detection of enterovirus in the islet cells of patients with type 1 diabetes: what do we learn from immunohistochemistry? Reply to Hansson SF, Korsgren S, Pontén F et al [letter]

  • Letter
  • Published:
Diabetologia Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Abbreviations

CKB:

Creatine kinase B

nPOD:

Network of Pancreatic Organ Donors with Diabetes

References

  1. Hansson SF, Korsgren S, Pontén F, Korsgren O (2013) Detection of enterovirus in the islet cells of patients with type 1 diabetes: what do we learn from immunohistochemistry? Diabetologia. doi:10.1007/s00125-013-3138-z

    PubMed  Google Scholar 

  2. Richardson SJ, Leete P, Dhayal S et al (2013) Evaluation of the fidelity of immunolabelling obtained with clone 5D8/1, a monoclonal antibody directed against the enteroviral capsid protein, VP1, in human pancreas. Diabetologia. doi:10.1007/s00125-013-3094-7

    Google Scholar 

  3. Campbell-Thompson M, Wasserfall C, Kaddis J et al (2012) Network for Pancreatic Organ Donors with Diabetes (nPOD): developing a tissue biobank for type 1 diabetes. Diabetes Metab Res Rev 28:608–617

    Article  PubMed Central  PubMed  Google Scholar 

  4. Richardson SJ, Willcox A, Bone AJ, Foulis AK, Morgan NG (2009) The prevalence of enteroviral capsid protein vp1 immunostaining in pancreatic islets in human type 1 diabetes. Diabetologia 52:1143–1151

    Article  CAS  PubMed  Google Scholar 

  5. Flodström M, Maday A, Balakrishna D, Cleary MM, Yoshimura A, Sarvetnick N (2002) Target cell defense prevents the development of diabetes after viral infection. Nat Immunol 3:373–382

    Article  PubMed  Google Scholar 

  6. Flodström-Tullberg M, Hultcrantz M, Stotland A et al (2005) RNase L and double-stranded RNA-dependent protein kinase exert complementary roles in islet cell defense during coxsackievirus infection. J Immunol 174:1171–1177

    PubMed  Google Scholar 

  7. Hansson SF, Korsgren S, Pontén F, Korsgren O (2013) Enteroviruses and the pathogenesis of type 1 diabetes revisited: cross-reactivity of enterovirus capsid protein (VP1) antibodies with human mitochondrial proteins. J Pathol 229:719–728

    Article  CAS  PubMed  Google Scholar 

  8. Sistermans EA, de Kok YJM, Peters W, Ginsel LA, Jap PHK, Wieringa B (1995) Tissue- and cell-specific distribution of creatine kinase B: a new highly specific monoclonal antibody for use in immunohistochemistry. Cell Tissue Res 280:435–446

    Article  CAS  PubMed  Google Scholar 

Download references

Funding

The work was supported by funding from the European Union’s Seventh Framework Programme PEVNET (FP7/2007-2013) under grant agreement number 261441. Additional support was from a Diabetes Research Wellness Foundation Non-Clinical Research Fellowship to SJR and from Karolinska Institutet (KL), the Strategic Research Programme in Diabetes at Karolinska Institutet (MF-T) and the Swedish Research Council (MF-T). The research was also performed with the support of the Network for Pancreatic Organ Donors with Diabetes (nPOD), a collaborative type 1 diabetes research project sponsored by the JDRF and with a JDRF research grant awarded to the nPOD-V Consortium. Organ procurement organisations (OPO) partnering with nPOD to provide research resources are listed at www.jdrfnpod.org/our-partners.php.

Duality of interest

HH is a minor (<5%) shareholder and member of the board of Vactech, which develops vaccines against picornaviruses. All other authors declare that there is no duality of interest associated with this manuscript.

Contribution statement

All authors were responsible for the conception and design of the manuscript and revising it critically for important intellectual content. All authors approved the version to be published.

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Sarah J. Richardson or Noel G. Morgan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Richardson, S.J., Leete, P., Dhayal, S. et al. Detection of enterovirus in the islet cells of patients with type 1 diabetes: what do we learn from immunohistochemistry? Reply to Hansson SF, Korsgren S, Pontén F et al [letter]. Diabetologia 57, 647–649 (2014). https://doi.org/10.1007/s00125-014-3167-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00125-014-3167-2

Keywords

Navigation