Abbreviations
- CKB:
-
Creatine kinase B
- nPOD:
-
Network of Pancreatic Organ Donors with Diabetes
References
Hansson SF, Korsgren S, Pontén F, Korsgren O (2013) Detection of enterovirus in the islet cells of patients with type 1 diabetes: what do we learn from immunohistochemistry? Diabetologia. doi:10.1007/s00125-013-3138-z
Richardson SJ, Leete P, Dhayal S et al (2013) Evaluation of the fidelity of immunolabelling obtained with clone 5D8/1, a monoclonal antibody directed against the enteroviral capsid protein, VP1, in human pancreas. Diabetologia. doi:10.1007/s00125-013-3094-7
Campbell-Thompson M, Wasserfall C, Kaddis J et al (2012) Network for Pancreatic Organ Donors with Diabetes (nPOD): developing a tissue biobank for type 1 diabetes. Diabetes Metab Res Rev 28:608–617
Richardson SJ, Willcox A, Bone AJ, Foulis AK, Morgan NG (2009) The prevalence of enteroviral capsid protein vp1 immunostaining in pancreatic islets in human type 1 diabetes. Diabetologia 52:1143–1151
Flodström M, Maday A, Balakrishna D, Cleary MM, Yoshimura A, Sarvetnick N (2002) Target cell defense prevents the development of diabetes after viral infection. Nat Immunol 3:373–382
Flodström-Tullberg M, Hultcrantz M, Stotland A et al (2005) RNase L and double-stranded RNA-dependent protein kinase exert complementary roles in islet cell defense during coxsackievirus infection. J Immunol 174:1171–1177
Hansson SF, Korsgren S, Pontén F, Korsgren O (2013) Enteroviruses and the pathogenesis of type 1 diabetes revisited: cross-reactivity of enterovirus capsid protein (VP1) antibodies with human mitochondrial proteins. J Pathol 229:719–728
Sistermans EA, de Kok YJM, Peters W, Ginsel LA, Jap PHK, Wieringa B (1995) Tissue- and cell-specific distribution of creatine kinase B: a new highly specific monoclonal antibody for use in immunohistochemistry. Cell Tissue Res 280:435–446
Funding
The work was supported by funding from the European Union’s Seventh Framework Programme PEVNET (FP7/2007-2013) under grant agreement number 261441. Additional support was from a Diabetes Research Wellness Foundation Non-Clinical Research Fellowship to SJR and from Karolinska Institutet (KL), the Strategic Research Programme in Diabetes at Karolinska Institutet (MF-T) and the Swedish Research Council (MF-T). The research was also performed with the support of the Network for Pancreatic Organ Donors with Diabetes (nPOD), a collaborative type 1 diabetes research project sponsored by the JDRF and with a JDRF research grant awarded to the nPOD-V Consortium. Organ procurement organisations (OPO) partnering with nPOD to provide research resources are listed at www.jdrfnpod.org/our-partners.php.
Duality of interest
HH is a minor (<5%) shareholder and member of the board of Vactech, which develops vaccines against picornaviruses. All other authors declare that there is no duality of interest associated with this manuscript.
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All authors were responsible for the conception and design of the manuscript and revising it critically for important intellectual content. All authors approved the version to be published.
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Richardson, S.J., Leete, P., Dhayal, S. et al. Detection of enterovirus in the islet cells of patients with type 1 diabetes: what do we learn from immunohistochemistry? Reply to Hansson SF, Korsgren S, Pontén F et al [letter]. Diabetologia 57, 647–649 (2014). https://doi.org/10.1007/s00125-014-3167-2
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DOI: https://doi.org/10.1007/s00125-014-3167-2