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. 2020 Dec 7:62:e97.
doi: 10.1590/S1678-9946202062097. eCollection 2020.

Anti-Zika virus activity of several abietane-type ferruginol analogues

Francielle T G Sousa  1   2 , Cristina Nunes  2 , Camila Malta Romano  2   3   4 , Ester Cerdeira Sabino  2   4 , Miguel Angel González-Cardenete  5
Affiliations

Anti-Zika virus activity of several abietane-type ferruginol analogues

Francielle T G Sousa et al. Rev Inst Med Trop Sao Paulo. .

Abstract

Abietane diterpenoids are naturally occurring plant metabolites with a broad spectrum of biological effects including antibacterial, antileishmanial, antitumor, antioxidant, as well as antiinflammatory activities. Recently, we found that some analogues of natural ferruginol ( 2 ) actively inhibited dengue virus 2 (DENV-2) replication. Due to the similarity with DENV, we envisaged that abietane diterpenoids would also be active against Zika virus (ZIKV). Six selected semi-synthetic abietane derivatives of (+)-dehydroabietylamine ( 3 ) were tested. Cytotoxicity was determined by MTT assay in Vero cells. In vitro anti-ZIKV (clinical isolate, IMT17) activity was evaluated by plaque assay. Interestingly, these molecules showed potential as anti-ZIKV agents, with EC50 values ranging from 0.67 to 18.57 µM, and cytotoxicity (CC50 values) from 2.56 to 35.09 µM. The 18-Oxoferruginol (8) (EC50 = 2.60 µM, SI = 13.51) and 12-nitro-N-benzoyldehydroabietylamine (9) (EC50= 0.67 µM, SI = 3.82) were the most active compounds, followed by 12-hydroxy-N-tosyldehydroabietylamine ( 7 ) (EC50 = 3.58 µM, SI = 3.20) and 12-hydroxy-N,N-phthaloyldehydroabietylamine ( 5 ) (EC50 = 7.76 µM, SI = 1.23). To the best of our knowledge, this is the first report on anti-Zika virus properties of abietanes.

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Conflict of interest statement

CONFLICT OF INTERESTS

The authors have no conflict of interests to disclose.

Figures

Figure 1
Figure 1. Some typical bioactive abietane-type diterpenoids.
Figure 2
Figure 2. Abietane analogues selected to test anti-Zika virus activity.
See this image and copyright information in PMC

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