Untargeted global metabolomic profiling of healthy dogs grouped on the basis of grain inclusivity of their diet and of dogs with subclinical cardiac abnormalities that underwent a diet change
- PMID: 35895762
- DOI: 10.2460/ajvr.22.03.0054
Untargeted global metabolomic profiling of healthy dogs grouped on the basis of grain inclusivity of their diet and of dogs with subclinical cardiac abnormalities that underwent a diet change
Abstract
Objective: To compare metabolomic profiles of dogs eating grain-free (GF) versus grain-inclusive (GI) diets (1) for healthy dogs at baseline and (2) for dogs with subclinical cardiac abnormalities at 12 months after a diet change.
Sample: Serum samples from 23 dogs eating GF diets and 79 dogs eating GI diets, of which 17 (8 eating a GF diet and 9 eating a GI diet) were reevaluated 12 months after a diet change.
Procedures: Metabolomic profiles were developed by means of ultrahigh-performance liquid chromatography-tandem mass spectroscopy of serum samples. Baseline results for the GF group were compared with those for the GI group. Dogs from both groups with subclinical cardiac abnormalities were transitioned to a GI, pulse-free, intervention diet, and samples collected 12 months later were compared between diet groups. Statistical significance for biochemical group differences was defined as P < .05 with a false discovery rate (q) < .10.
Results: Baseline differences in lipid metabolism and amino acid metabolism were found between the GF and GI diet groups. There were 46 metabolites that were higher and 82 metabolites that were lower in the GF group (n = 23), compared with the GI group (79). Comparison of the GF (n = 8) and GI (9) groups 12 months after the diet change showed only 6 metabolites that were higher and 11 metabolites that were lower in the GF group, compared with the GI group.
Clinical relevance: Metabolomic pathway differences between dogs eating GF versus GI diets highlight the important effect of diet in metabolomics analyses. The clinical importance of these differences and how they might relate to cardiac disease in dogs remains undetermined.
Similar articles
-
Effect of diet change in healthy dogs with subclinical cardiac biomarker or echocardiographic abnormalities.J Vet Intern Med. 2022 May;36(3):1057-1065. doi: 10.1111/jvim.16416. Epub 2022 Apr 14. J Vet Intern Med. 2022. PMID: 35420218 Free PMC article.
-
Effect of type of diet on blood and plasma taurine concentrations, cardiac biomarkers, and echocardiograms in 4 dog breeds.J Vet Intern Med. 2021 Mar;35(2):771-779. doi: 10.1111/jvim.16075. Epub 2021 Feb 27. J Vet Intern Med. 2021. PMID: 33638176 Free PMC article.
-
Echocardiographic phenotype of canine dilated cardiomyopathy differs based on diet type.J Vet Cardiol. 2019 Feb;21:1-9. doi: 10.1016/j.jvc.2018.11.002. Epub 2018 Dec 5. J Vet Cardiol. 2019. PMID: 30797439
-
Translational Metabolomics of Head Injury: Exploring Dysfunctional Cerebral Metabolism with Ex Vivo NMR Spectroscopy-Based Metabolite Quantification.In: Kobeissy FH, editor. Brain Neurotrauma: Molecular, Neuropsychological, and Rehabilitation Aspects. Boca Raton (FL): CRC Press/Taylor & Francis; 2015. Chapter 25. In: Kobeissy FH, editor. Brain Neurotrauma: Molecular, Neuropsychological, and Rehabilitation Aspects. Boca Raton (FL): CRC Press/Taylor & Francis; 2015. Chapter 25. PMID: 26269925 Free Books & Documents. Review.
-
Are Gluten-Free Diets More Nutritious? An Evaluation of Self-Selected and Recommended Gluten-Free and Gluten-Containing Dietary Patterns.Nutrients. 2018 Dec 3;10(12):1881. doi: 10.3390/nu10121881. Nutrients. 2018. PMID: 30513876 Free PMC article. Review.
Cited by
-
Comparison of echocardiography, biomarkers and taurine concentrations in cats eating high- or low-pulse diets.J Feline Med Surg. 2023 Feb;25(2):1098612X231154859. doi: 10.1177/1098612X231154859. J Feline Med Surg. 2023. PMID: 36803067 Free PMC article.
MeSH terms
LinkOut - more resources
Full Text Sources