Quantification of spatiotemporal patterns of Ras isoform expression during development
- PMID: 28117393
- PMCID: PMC5259795
- DOI: 10.1038/srep41297
Quantification of spatiotemporal patterns of Ras isoform expression during development
Abstract
Ras proteins are important signalling hubs frequently dysregulated in cancer and in a group of developmental disorders called Rasopathies. Three Ras genes encode four proteins that differentially contribute to these phenotypes. Using quantitative real-time PCR (qRT-PCR) we have measured the gene expression profiles of each of the Ras isoforms in a panel of mouse tissues derived from a full developmental time course spanning embryogenesis through to adulthood. In most tissues and developmental stages we observe a relative contribution of KRas4B > > NRas ≥ KRas4A > HRas to total Ras expression with KRas4B typically representing 60-99% of all Ras transcripts. KRas4A is the most dynamically regulated Ras isoform with significant up-regulation of expression observed pre-term in stomach, intestine, kidney and heart. The expression patterns assist interpretation of the essential role of KRas in development and the preponderance of KRas mutations in cancer.
Figures
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![Figure 3](https://cdn.statically.io/img/www.ncbi.nlm.nih.gov/pmc/articles/instance/5259795/bin/srep41297-f3.gif)
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References
-
- Koera K. et al.. K-ras is essential for the development of the mouse embryo. Oncogene 15, 1151–1159 (1997). - PubMed
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