The Neuroprotective Properties of Hericium erinaceus in Glutamate-Damaged Differentiated PC12 Cells and an Alzheimer's Disease Mouse Model
- PMID: 27809277
- PMCID: PMC5133811
- DOI: 10.3390/ijms17111810
The Neuroprotective Properties of Hericium erinaceus in Glutamate-Damaged Differentiated PC12 Cells and an Alzheimer's Disease Mouse Model
Abstract
Hericium erinaceus, an edible and medicinal mushroom, displays various pharmacological activities in the prevention of dementia in conditions such as Parkinson's and Alzheimer's disease. The present study explored the neuroprotective effects of H. erinaceus mycelium polysaccharide-enriched aqueous extract (HE) on an l-glutamic acid (l-Glu)-induced differentiated PC12 (DPC12) cellular apoptosis model and an AlCl₃ combined with d-galactose-induced Alzheimer's disease mouse model. The data revealed that HE successfully induced PC12 cell differentiation. A 3 h HE incubation at doses of 50 and 100 µg/mL before 25 mM of l-Glu effectively reversed the reduction of cell viability and the enhancement of the nuclear apoptosis rate in DPC12 cells. Compared with l-Glu-damaged cells, in PC12 cells, HE suppressed intracellular reactive oxygen species accumulation, blocked Ca2+ overload and prevented mitochondrial membrane potential (MMP) depolarization. In the Alzheimer's disease mouse model, HE administration enhanced the horizontal and vertical movements in the autonomic activity test, improved the endurance time in the rotarod test, and decreased the escape latency time in the water maze test. It also improved the central cholinergic system function in the Alzheimer's mice, demonstrated by the fact that it dose-dependently enhanced the acetylcholine (Ach) and choline acetyltransferase (ChAT) concentrations in both the serum and the hypothalamus. Our findings provide experimental evidence that HE may provide neuroprotective candidates for treating or preventing neurodegenerative diseases.
Keywords: Alzheimer‘s disease mouse; Hericium erinaceus; glutamate; mitochondria; neuro-protection.
Conflict of interest statement
The authors declare no conflict of interest.
Figures
![Figure 1](https://cdn.statically.io/img/www.ncbi.nlm.nih.gov/pmc/articles/instance/5133811/bin/ijms-17-01810-g001.gif)
![Figure 2](https://cdn.statically.io/img/www.ncbi.nlm.nih.gov/pmc/articles/instance/5133811/bin/ijms-17-01810-g002.gif)
![Figure 3](https://cdn.statically.io/img/www.ncbi.nlm.nih.gov/pmc/articles/instance/5133811/bin/ijms-17-01810-g003a.gif)
![Figure 3](https://cdn.statically.io/img/www.ncbi.nlm.nih.gov/pmc/articles/instance/5133811/bin/ijms-17-01810-g003a.gif)
![Figure 4](https://cdn.statically.io/img/www.ncbi.nlm.nih.gov/pmc/articles/instance/5133811/bin/ijms-17-01810-g004.gif)
![Figure 5](https://cdn.statically.io/img/www.ncbi.nlm.nih.gov/pmc/articles/instance/5133811/bin/ijms-17-01810-g005.gif)
![Figure 6](https://cdn.statically.io/img/www.ncbi.nlm.nih.gov/pmc/articles/instance/5133811/bin/ijms-17-01810-g006.gif)
Similar articles
-
Anti-Oxidative Stress Activity Is Essential for Amanita caesarea Mediated Neuroprotection on Glutamate-Induced Apoptotic HT22 Cells and an Alzheimer's Disease Mouse Model.Int J Mol Sci. 2017 Jul 27;18(8):1623. doi: 10.3390/ijms18081623. Int J Mol Sci. 2017. PMID: 28749416 Free PMC article.
-
Investigation of the neuroprotective effects of Lycium barbarum water extract in apoptotic cells and Alzheimer's disease mice.Mol Med Rep. 2018 Mar;17(3):3599-3606. doi: 10.3892/mmr.2017.8310. Epub 2017 Dec 19. Mol Med Rep. 2018. PMID: 29257339 Free PMC article.
-
Mitochondria Related Pathway Is Essential for Polysaccharides Purified from Sparassis crispa Mediated Neuro-Protection against Glutamate-Induced Toxicity in Differentiated PC12 Cells.Int J Mol Sci. 2016 Jan 26;17(2):133. doi: 10.3390/ijms17020133. Int J Mol Sci. 2016. PMID: 26821016 Free PMC article.
-
Neurohealth Properties of Hericium erinaceus Mycelia Enriched with Erinacines.Behav Neurol. 2018 May 21;2018:5802634. doi: 10.1155/2018/5802634. eCollection 2018. Behav Neurol. 2018. PMID: 29951133 Free PMC article. Review.
-
The potential therapeutic role of Hericium erinaceus extract in pathologic conditions involving the urogenital-gut axis: insights into the involved mechanisms and mediators.J Physiol Pharmacol. 2024 Feb;75(1). doi: 10.26402/jpp.2024.1.01. Epub 2024 Apr 3. J Physiol Pharmacol. 2024. PMID: 38583434 Review.
Cited by
-
Brozopine ameliorates cognitive impairment via upregulating Nrf2, antioxidation and anti-inflammation activities.Front Pharmacol. 2024 Jul 10;15:1428455. doi: 10.3389/fphar.2024.1428455. eCollection 2024. Front Pharmacol. 2024. PMID: 39050756 Free PMC article.
-
In vitro assessment of multipotential therapeutic importance of Hericium erinaceus mushroom extracts using different solvents.Bioresour Bioprocess. 2022 Sep 16;9(1):99. doi: 10.1186/s40643-022-00592-6. Bioresour Bioprocess. 2022. PMID: 38647811 Free PMC article.
-
Neurotrophic and Neuroprotective Effects of Hericium erinaceus.Int J Mol Sci. 2023 Nov 3;24(21):15960. doi: 10.3390/ijms242115960. Int J Mol Sci. 2023. PMID: 37958943 Free PMC article. Review.
-
Multiple Metabolites Derived from Mushrooms and Their Beneficial Effect on Alzheimer's Diseases.Nutrients. 2023 Jun 15;15(12):2758. doi: 10.3390/nu15122758. Nutrients. 2023. PMID: 37375662 Free PMC article. Review.
-
Hericium erinaceus in Neurodegenerative Diseases: From Bench to Bedside and Beyond, How Far from the Shoreline?J Fungi (Basel). 2023 May 10;9(5):551. doi: 10.3390/jof9050551. J Fungi (Basel). 2023. PMID: 37233262 Free PMC article. Review.
References
-
- Chang C.H., Chen Y., Yew X.X., Chen H.X., Kim J.X., Chang C.C., Peng C.C., Peng R.Y. Improvement of erinacine a productivity in Hericium erinaceus mycelia and its neuroprotective bioactivity against the glutamate-insulted apoptosis. LWT-Food Sci. Technol. 2016;65:1100–1108. doi: 10.1016/j.lwt.2015.08.014. - DOI
-
- Rosello A., Warnes G., Meier U.C. Cell death pathways and autophagy in the central nervous system and its involvement in neurodegeneration, immunity and central nervous system infection: To die or not to die—That is the question. Clin. Exp. Immunol. 2012;168:52–57. doi: 10.1111/j.1365-2249.2011.04544.x. - DOI - PMC - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Miscellaneous