RNA Sequencing of Single Human Islet Cells Reveals Type 2 Diabetes Genes
- PMID: 27667665
- DOI: 10.1016/j.cmet.2016.08.018
RNA Sequencing of Single Human Islet Cells Reveals Type 2 Diabetes Genes
Abstract
Pancreatic islet cells are critical for maintaining normal blood glucose levels, and their malfunction underlies diabetes development and progression. We used single-cell RNA sequencing to determine the transcriptomes of 1,492 human pancreatic α, β, δ, and PP cells from non-diabetic and type 2 diabetes organ donors. We identified cell-type-specific genes and pathways as well as 245 genes with disturbed expression in type 2 diabetes. Importantly, 92% of the genes have not previously been associated with islet cell function or growth. Comparison of gene profiles in mouse and human α and β cells revealed species-specific expression. All data are available for online browsing and download and will hopefully serve as a resource for the islet research community.
Keywords: glucagon; insulin; pancreatic islet cell; pancreatic polypeptide; single-cell RNA sequencing; somatostatin; type 2 diabetes.
Copyright © 2016 Elsevier Inc. All rights reserved.
Comment in
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Single-Cell Sequencing of Human Pancreatic Islets-New Kids on the Block.Cell Metab. 2016 Oct 11;24(4):523-524. doi: 10.1016/j.cmet.2016.09.012. Cell Metab. 2016. PMID: 27732832
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Diabetes: Transcriptomes reveal specific islet cell signatures.Nat Rev Endocrinol. 2016 Dec;12(12):686. doi: 10.1038/nrendo.2016.173. Epub 2016 Oct 14. Nat Rev Endocrinol. 2016. PMID: 27739516 No abstract available.
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