Free Radical Scavenging Properties of Skin and Pulp Extracts of Different Grape Cultivars In Vitro and Attenuation of H2O2-Induced Oxidative Stress in Liver Tissue Ex Vivo
- PMID: 26089617
- PMCID: PMC4469050
- DOI: 10.1007/s12291-014-0442-4
Free Radical Scavenging Properties of Skin and Pulp Extracts of Different Grape Cultivars In Vitro and Attenuation of H2O2-Induced Oxidative Stress in Liver Tissue Ex Vivo
Abstract
Grapes are the richest source of antioxidants due to the presence of potent bioactive phytochemicals. In this study, the phytochemical contents, scavenging activities and protective role against H2O2-induced oxidative stress in liver tissue ex vivo of four grape (Vitis vinifera) cultivars extracts, namely Flame seedless (black), Kishmish chorni (black with reddish brown), Red globe (red) and Thompson seedless mutant (green), were evaluated. The total phenolics and flavonoids content in pulp or skin fractions of different grape cultivars were in the range of 47.6-310 mg gallic acid equivalent/g fresh weight (fw), and 46.6-733.3 µg catechin equivalent/g fw respectively. The scavenging activities in skin of different grape varieties against 2,2-diphenyl-1-picrylhydrazyl (44-58 %), hydrogen peroxide (15.3-18.6 %), and hydroxyl radicals (50-85 %), were higher than pulp of the corresponding cultivars. These scavenging activities of grape extracts were found to be significantly (p < 0.01) correlated with the levels of total phenols, flavonoids and ascorbic acid. Liver tissues from goat treated with H2O2 (500 μM) showed significantly decreased GSH content by 42.9 % and activities of catalase by 50 % and glutathione reductase by 66.6 %; while increased thiobarbituric acid reactive substances and nitric oxide level by 2.53- and 0.86-fold, respectively, and activity of glutathione S-transferase by 0.96-fold. Grape skin extracts showed the stronger protective activity against H2O2-induced oxidative stress in liver tissue ex vivo, than its pulp of any cultivar; and the Flame seedless (black) cultivar showed the highest potential. In conclusion, our study suggested that the higher antioxidant potential, phytochemical contents and significant scavenging capacities in pulp and skin of grape extracts showed the protective action of grape extracts against H2O2-induced oxidative stress in liver tissue ex vivo.
Keywords: Antioxidant; Grapes; Hydrogen peroxide; Liver; Scavenging activity; Vitis vinifera.
Similar articles
-
Grape Seed.2023 Mar 20. LiverTox: Clinical and Research Information on Drug-Induced Liver Injury [Internet]. Bethesda (MD): National Institute of Diabetes and Digestive and Kidney Diseases; 2012–. 2023 Mar 20. LiverTox: Clinical and Research Information on Drug-Induced Liver Injury [Internet]. Bethesda (MD): National Institute of Diabetes and Digestive and Kidney Diseases; 2012–. PMID: 37043594 Free Books & Documents. Review.
-
Grape extract protects against ionizing radiation-induced membrane alterations in Red Blood Cells.Indian J Exp Biol. 2016 Nov;54(11):735-44. Indian J Exp Biol. 2016. PMID: 30179398
-
Phytochemical Constituents, Health Benefits, and Industrial Applications of Grape Seeds: A Mini-Review.Antioxidants (Basel). 2017 Sep 15;6(3):71. doi: 10.3390/antiox6030071. Antioxidants (Basel). 2017. PMID: 28914789 Free PMC article. Review.
-
Scavenging and antioxidant properties of different grape cultivars against ionizing radiation-induced liver damage ex vivo.Indian J Exp Biol. 2016 Apr;54(4):280-5. Indian J Exp Biol. 2016. PMID: 27295925
-
Antioxidant potential of different grape cultivars against Fenton-like reagent-induced liver damage ex-vivo.Indian J Biochem Biophys. 2014 Oct;51(5):372-7. Indian J Biochem Biophys. 2014. PMID: 25630107
Cited by
-
Aging, oxidative stress and degenerative diseases: mechanisms, complications and emerging therapeutic strategies.Biogerontology. 2023 Oct;24(5):609-662. doi: 10.1007/s10522-023-10050-1. Epub 2023 Jul 30. Biogerontology. 2023. PMID: 37516673 Review.
-
Antioxidant Properties of Trianthema Portulacastrum and Protection Against Ionizing Radiation-Induced Liver Damage Ex vivo.Indian J Clin Biochem. 2022 Apr;37(2):192-198. doi: 10.1007/s12291-021-00964-3. Epub 2021 Mar 8. Indian J Clin Biochem. 2022. PMID: 35463107 Free PMC article.
-
Characterization of the Omija (Schisandra chinensis) Extract and Its Effects on the Bovine Sperm Vitality and Oxidative Profile during In Vitro Storage.Evid Based Complement Alternat Med. 2020 Sep 21;2020:7123780. doi: 10.1155/2020/7123780. eCollection 2020. Evid Based Complement Alternat Med. 2020. PMID: 33029171 Free PMC article.
-
A Phenolic Acid and Flavonoid Fraction Isolated from Lolium multiflorum Lam. Prevents d-Galactosamine-Induced Liver Damages through the Augmentation of Nrf2 Expression.Indian J Clin Biochem. 2019 Jan;34(1):68-75. doi: 10.1007/s12291-017-0714-x. Epub 2017 Dec 2. Indian J Clin Biochem. 2019. PMID: 30728675 Free PMC article.
-
Dietary Phytochemicals Promote Health by Enhancing Antioxidant Defence in a Pig Model.Nutrients. 2017 Jul 14;9(7):758. doi: 10.3390/nu9070758. Nutrients. 2017. PMID: 28708113 Free PMC article.
References
-
- Inoue M, Sato EF, Nishikawa M, Park AM, Kira Y, Imada I, Utsumi K. Cross talk of nitric oxide, oxygen radicals, and superoxide dismutase regulates the energy metabolism and cell death and determines the fates of aerobic life. Antioxid Redox Signal. 2003;5(4):475–484. doi: 10.1089/152308603768295221. - DOI - PubMed
-
- Reddy SV, Suchitra MM, Reddy YM, Reddy PE. Beneficial and detrimental actions of free radicals: a review. J Global Pharma Technol. 2010;2:3–11.
LinkOut - more resources
Full Text Sources
Other Literature Sources