Development and function of human innate immune cells in a humanized mouse model
- PMID: 24633240
- PMCID: PMC4017589
- DOI: 10.1038/nbt.2858
Development and function of human innate immune cells in a humanized mouse model
Erratum in
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Corrigendum: Development and function of human innate immune cells in a humanized mouse model.Nat Biotechnol. 2017 Dec 8;35(12):1211. doi: 10.1038/nbt1217-1211c. Nat Biotechnol. 2017. PMID: 29220023 No abstract available.
Abstract
Mice repopulated with human hematopoietic cells are a powerful tool for the study of human hematopoiesis and immune function in vivo. However, existing humanized mouse models cannot support development of human innate immune cells, including myeloid cells and natural killer (NK) cells. Here we describe two mouse strains called MITRG and MISTRG, in which human versions of four genes encoding cytokines important for innate immune cell development are knocked into their respective mouse loci. The human cytokines support the development and function of monocytes, macrophages and NK cells derived from human fetal liver or adult CD34(+) progenitor cells injected into the mice. Human macrophages infiltrated a human tumor xenograft in MITRG and MISTRG mice in a manner resembling that observed in tumors obtained from human patients. This humanized mouse model may be used to model the human immune system in scenarios of health and pathology, and may enable evaluation of therapeutic candidates in an in vivo setting relevant to human physiology.
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Comment in
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New models of human immunity.Nat Biotechnol. 2014 Apr;32(4):335-6. doi: 10.1038/nbt.2871. Nat Biotechnol. 2014. PMID: 24714481 No abstract available.
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